Immuno–spin trapping of DNA radicals

2006 ◽  
Vol 3 (2) ◽  
pp. 123-127 ◽  
Author(s):  
Dario C Ramirez ◽  
Sandra E Gomez Mejiba ◽  
Ronald P Mason
Keyword(s):  
Spin Trapping ◽  
Dna Radicals

scholarly journals Immuno-spin trapping analyses of DNA radicals

2007 ◽  
Vol 2 (3) ◽  
pp. 512-522 ◽  
Author(s):  
Dario C Ramirez ◽  
Sandra E Gomez-Mejiba ◽  
Ronald P Mason
Keyword(s):  
Spin Trapping ◽  
Dna Radicals

New and Improved Immuno-Spin Trapping Methods for the Detection of DNA Radicals

2011 ◽  
Vol 51 ◽  
pp. S31-S32
Author(s):  
Fiona A. Summers ◽  
Ronald P. Mason ◽  
Marilyn Ehrenshaft
Keyword(s):  
Spin Trapping ◽  
Dna Radicals ◽  
Trapping Methods

scholarly journals Immuno-spin trapping of protein and DNA radicals: “Tagging” free radicals to locate and understand the redox process

2009 ◽  
Vol 46 (7) ◽  
pp. 853-865 ◽  
Author(s):  
Sandra E. Gomez-Mejiba ◽  
Zili Zhai ◽  
Hammad Akram ◽  
Leesa J. Deterding ◽  
Kenneth Hensley ◽  
...  
Keyword(s):  
Free Radicals ◽  
Spin Trapping ◽  
Redox Process ◽  
Dna Radicals

scholarly journals DNA Cleavage and Detection of DNA Radicals Formed from Hydralazine and Copper (II) by ESR and Immuno-Spin Trapping

2014 ◽  
Vol 27 (4) ◽  
pp. 674-682 ◽  
Author(s):  
Birandra K. Sinha ◽  
Fabian Leinisch ◽  
Suchandra Bhattacharjee ◽  
Ronald P. Mason
Keyword(s):  
Dna Cleavage ◽  
Spin Trapping ◽  
Dna Radicals

Enhancement of hydroxyl radical generation by phenols and their reaction intermediates during ozonation

1998 ◽  
Vol 38 (6) ◽  
pp. 147-154 ◽  
Author(s):  
Hideo Utsumi ◽  
Sang-Kuk Han ◽  
Kazuhiro Ichikawa
Keyword(s):  
Hydroxyl Radical ◽  
Hydroxyl Radicals ◽  
Spin Trapping ◽  
Active Species ◽  
Generation Rate ◽  
Peak Height Ratio ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Phenol Derivatives ◽  
Semiquinone Radicals

Generation of hydroxyl radicals, one of the major active species in ozonation of water was directly observed with a spin-trapping/electron spin resonance (ESR) technique using 5,5-dimethyl-1-pyrrolineN-oxide (DMPO) as a spin-trapping reagent. Hydroxyl radical were trapped with DMPO as a stable radical, DMPO-OH. Eighty μM of ozone produced 1.08 X 10-6M of DMPO-OH, indicating that 1.4% of •OH is trapped with DMPO. Generation rate of DMPO-OH was determined by ESR/stopped-flow measurement. Phenol derivatives increased the amount and generation rate of DMPO-OH, indicating that phenol derivatives enhance •OH generation during ozonation of water. Ozonation of 2,3-, 2,5-, 2,6-dichlorophenol gave an ESR spectra of triplet lines whose peak height ratio were 1:2:1. ESR parameters of the triplet lines agreed with those of the corresponding dichloro-psemiquinone radical. Ozonation of 2,4,5- and 2,4,6-trichlorophenol gave the same spectra as those of 2,5- and 2,6-dichlorophenol, respectively, indicating that a chlorine group in p-position is substituted with a hydroxy group during ozonation. Amounts of the radical increased in an ozone-concentration dependent manner and were inhibited by addition of hydroxyl radical scavengers. These results suggest that p-semiquinone radicals are generated from the chlorophenols by hydroxyl radicals during ozonation. The p-semiquinone radicals were at least partly responsible for enhancements of DMPO-OH generation.

Photoredox Reactions of Iodo Iron(III) Complexes Containing Tetradentate Ligands

2001 ◽  
Vol 66 (1) ◽  
pp. 109-118 ◽  
Author(s):  
Jozef Šima ◽  
Dáša Lauková ◽  
Vlasta Brezová
Keyword(s):  
Schiff Bases ◽  
Spin Trapping ◽  
Molar Ratio ◽  
Oh Radicals ◽  
Solvated Electrons ◽  
Open Chain ◽  
Tetradentate Ligands ◽  
Visible Irradiation ◽  
Epr Spin Trapping ◽  
Spin Trapping Technique

Photoredox reactions occurring in irradiated methanolic solutions of trans-[FeIII(R,R'-salen)(CH3OH)I], where R,R'-salen2- are N,N'-ethylenebis(R,R'-salicylideneiminato), tetradentate open-chain N2O2-Schiff bases with R,R' = H, 5-Cl, 5-Br, 3,5-di-Br, 3,5-di-(CH3), 3-OCH3, 5-OCH3, have been investigated and their mechanism proposed. The complexes are redox-stable in the dark. Ultraviolet and/or visible irradiation of methanolic solution of the complexes induces photoreduction of Fe(III) to Fe(II). Depending on the composition of the irradiated solutions, •CH2OH radicals or solvated electrons were identified by the EPR spin trapping technique. The final product of the photooxidation coupled with the photoreduction of Fe(III) is formaldehyde and the molar ratio of Fe(II) and CH2O is close to 2 : 1. The efficiency of the photoredox process is strongly wavelength-dependent and influenced by the peripheral groups R,R' of the tetradentate ligands.

In Vivo Spin Trapping of Glyceryl Trinitrate–Derived Nitric Oxide in Rabbit Blood Vessels and Organs

Circulation ◽  
1995 ◽  
Vol 92 (7) ◽  
pp. 1876-1882 ◽  
Author(s):  
Alexander Mülsch ◽  
Peter Mordvintcev ◽  
Eberhard Bassenge ◽  
Frank Jung ◽  
Bernd Clement ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Glyceryl Trinitrate ◽  
Blood Vessels ◽  
Spin Trapping ◽  
Rabbit Blood
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