scholarly journals DNA Cleavage and Detection of DNA Radicals Formed from Hydralazine and Copper (II) by ESR and Immuno-Spin Trapping

2014 ◽  
Vol 27 (4) ◽  
pp. 674-682 ◽  
Author(s):  
Birandra K. Sinha ◽  
Fabian Leinisch ◽  
Suchandra Bhattacharjee ◽  
Ronald P. Mason
Keyword(s):  
Dna Cleavage ◽  
Spin Trapping ◽  
Dna Radicals

Structural Variety of Copper(II)-Peroxide Adducts and Its Relevance to DNA Cleavage

1999 ◽  
Vol 54 (1-2) ◽  
pp. 94-99 ◽  
Author(s):  
Satoshi Nishino ◽  
Teruyuki Kobayashi ◽  
Mami Kunita ◽  
Sayo Ito ◽  
Yuzo Nishida
Keyword(s):  
Hydrogen Peroxide ◽  
High Activity ◽  
Dna Cleavage ◽  
Esr Spectroscopy ◽  
Spin Trapping ◽  
The Other ◽  
Experimental Conditions ◽  
Structural Variety ◽  
Supercoiled Form ◽  
Tetradentate Ligands

The reactivity of copper(II) compounds with several tetradentate ligands towards some spin-trapping reagents was studied in the presence of hydrogen peroxide. The compounds used in this study are roughly divided into two groups based on the reactivity towards 2 ,2 ,6 ,6 -tetramethyl-4-piperidinol(and also 2,2,6,6-tetramethyl-4-piperidone), which are trapping agents for singlet oxygen, 1O2 (1Δg); The A-group compounds exhibited a high activity to form the corresponding nitrone radical, which was detected by ESR spectroscopy, but corresponding activity of the B-group compounds was very low. The A-group compounds defined as above exhibited high activity for cleavage of DNA(supercoiled Form I) in the presence of hydrogen peroxide, yielding DNA Form II (relaxed circular) or Form III (linear duplex) under our experimental conditions ([Cu(II)]=0.1~0.5 mᴍ). On the other hand, the B-group compounds effected complete degradation of the DNA (double-strand scission) under the same experimental conditions, formation of Form II or Form III DNA was negligible. Two different DNA cleavage patterns observed for A-and B-group compounds were elucidated by the different structural property of the copper(II)-peroxide adducts, which is controlled by the interaction through both DNA and the peripheral group of the ligand system

scholarly journals Immuno-spin trapping analyses of DNA radicals

2007 ◽  
Vol 2 (3) ◽  
pp. 512-522 ◽  
Author(s):  
Dario C Ramirez ◽  
Sandra E Gomez-Mejiba ◽  
Ronald P Mason
Keyword(s):  
Spin Trapping ◽  
Dna Radicals

Spin-trapping Study of DNA Cleavage Induced by Enediyne C-1027 Chromophore

2002 ◽  
Vol 31 (11) ◽  
pp. 1148-1149 ◽  
Author(s):  
Toyonobu Usuki ◽  
Masayuki Inoue ◽  
Kimio Akiyama ◽  
Masahiro Hirama
Keyword(s):  
Dna Cleavage ◽  
Spin Trapping

New and Improved Immuno-Spin Trapping Methods for the Detection of DNA Radicals

2011 ◽  
Vol 51 ◽  
pp. S31-S32
Author(s):  
Fiona A. Summers ◽  
Ronald P. Mason ◽  
Marilyn Ehrenshaft
Keyword(s):  
Spin Trapping ◽  
Dna Radicals ◽  
Trapping Methods

scholarly journals Immuno-spin trapping of protein and DNA radicals: “Tagging” free radicals to locate and understand the redox process

2009 ◽  
Vol 46 (7) ◽  
pp. 853-865 ◽  
Author(s):  
Sandra E. Gomez-Mejiba ◽  
Zili Zhai ◽  
Hammad Akram ◽  
Leesa J. Deterding ◽  
Kenneth Hensley ◽  
...  
Keyword(s):  
Free Radicals ◽  
Spin Trapping ◽  
Redox Process ◽  
Dna Radicals

Immuno–spin trapping of DNA radicals

2006 ◽  
Vol 3 (2) ◽  
pp. 123-127 ◽  
Author(s):  
Dario C Ramirez ◽  
Sandra E Gomez Mejiba ◽  
Ronald P Mason
Keyword(s):  
Spin Trapping ◽  
Dna Radicals
Sign in / Sign up

Export Citation Format

Share Document