Deep tissue two-photon microscopy

2005 ◽  
Vol 2 (12) ◽  
pp. 932-940 ◽  
Author(s):  
Fritjof Helmchen ◽  
Winfried Denk
Keyword(s):  
Deep Tissue ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals The Power of Single and Multibeam Two-Photon Microscopy for High-Resolution and High-Speed Deep Tissue and Intravital Imaging

2007 ◽  
Vol 93 (7) ◽  
pp. 2519-2529 ◽  
Author(s):  
Raluca Niesner ◽  
Volker Andresen ◽  
Jens Neumann ◽  
Heinrich Spiecker ◽  
Matthias Gunzer
Keyword(s):  
High Resolution ◽  
High Speed ◽  
Intravital Imaging ◽  
Deep Tissue ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Dual Near Infrared Two-Photon Microscopy for Deep-Tissue Dopamine Nanosensor Imaging

2017 ◽  
Author(s):  
Jackson T. Del Bonis-O’Donnell ◽  
Ralph H. Page ◽  
Abraham G. Beyene ◽  
Eric G. Tindall ◽  
Ian McFarlane ◽  
...  
Keyword(s):  
Near Infrared ◽  
Photon Absorption ◽  
Deep Tissue ◽  
Biologically Relevant ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Tissue Phantom ◽  
Photon Excitation ◽  
Photon Absorption And Scattering ◽  
Two Photon Excitation

A key limitation for achieving deep imaging in biological structures lies in photon absorption and scattering leading to attenuation of fluorescence. In particular, neurotransmitter imaging is challenging in the biologically-relevant context of the intact brain, for which photons must traverse the cranium, skin and bone. Thus, fluorescence imaging is limited to the surface cortical layers of the brain, only achievable with craniotomy. Herein, we describe optimal excitation and emission wavelengths for through-cranium imaging, and demonstrate that near-infrared emissive nanosensors can be photoexcited using a two-photon 1560 nm excitation source. Dopamine-sensitive nanosensors can undergo two-photon excitation, and provide chirality-dependent responses selective for dopamine with fluorescent turn-on responses varying between 20% and 350%. We further calculate the two-photon absorption cross-section and quantum yield of dopamine nanosensors, and confirm a two-photon power law relationship for the nanosensor excitation process. Finally, we show improved image quality of the nanosensors embedded 2 mm deep into a brain-mimetic tissue phantom, whereby one-photon excitation yields 42% scattering, in contrast to 4% scattering when the same object is imaged under two-photon excitation. Our approach overcomes traditional limitations in deep-tissue fluorescence microscopy, and can enable neurotransmitter imaging in the biologically-relevant milieu of the intact and living brain.

scholarly journals Addressing the autofluorescence issue in deep tissue imaging by two-photon microscopy: the significance of far-red emitting dyes

2017 ◽  
Vol 8 (11) ◽  
pp. 7696-7704 ◽  
Author(s):  
Yong Woong Jun ◽  
Hye Rim Kim ◽  
Ye Jin Reo ◽  
Mingchong Dai ◽  
Kyo Han Ahn
Keyword(s):  
Wavelength Region ◽  
Water Soluble ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Two Photon Microscopy ◽  
New Class ◽  
Two Photon ◽  
Deep Tissue Imaging

We have developed a new class of two-photon absorbing dyes that are far-red emitting, water-soluble, and very bright inside cells as well as in tissue. The significant autofluorescence from yellow wavelength region in tissue imaging can be addressed by deep-red emitting dyes.

scholarly journals Advantages of Non-degenerate Two-photon Microscopy for Deep Tissue Imaging

2020 ◽  
Vol 118 (3) ◽  
pp. 311a
Author(s):  
Sanaz Sadegh ◽  
Mu-Han Yang ◽  
Christopher Ferri ◽  
Martin Thunemann ◽  
Anna Devor ◽  
...  
Keyword(s):  
Tissue Imaging ◽  
Deep Tissue ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Deep Tissue Imaging

scholarly journals A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue

2015 ◽  
Vol 6 (3) ◽  
pp. 1944-1948 ◽  
Author(s):  
Pablo Rivera-Fuentes ◽  
Alexandra T. Wrobel ◽  
Melissa L. Zastrow ◽  
Mustafa Khan ◽  
John Georgiou ◽  
...  
Keyword(s):  
Fluorescent Sensor ◽  
Live Cell ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Deep Tissue Imaging ◽  
Acidic Vesicles ◽  
Unambiguous Detection

A fluorescent sensor that is pH-insensitive and compatible with two-photon microscopy was developed and applied to live cell and deep tissue imaging.

Does digital deconvolution improve two-photon microscopy in deep tissue imaging?

2001 ◽  
Author(s):  
Colten R. Noakes ◽  
Toshiyasu Goto ◽  
Raymond Keller ◽  
Ammasi Periasamy
Keyword(s):  
Tissue Imaging ◽  
Deep Tissue ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Deep Tissue Imaging

scholarly journals Erratum: Corrigendum: Deep tissue two-photon microscopy

2006 ◽  
Vol 3 (3) ◽  
pp. 235-235 ◽  
Author(s):  
Fritjof Helmchen ◽  
Winfried Denk
Keyword(s):  
Deep Tissue ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Dual Near-Infrared Two-Photon Microscopy for Deep-Tissue Dopamine Nanosensor Imaging

2017 ◽  
Vol 27 (39) ◽  
pp. 1702112 ◽  
Author(s):  
Jackson T. Del Bonis-O'Donnell ◽  
Ralph H. Page ◽  
Abraham G. Beyene ◽  
Eric G. Tindall ◽  
Ian R. McFarlane ◽  
...  
Keyword(s):  
Near Infrared ◽  
Deep Tissue ◽  
Two Photon Microscopy ◽  
Two Photon

Two-Photon Microscopy for Deep Tissue Imaging of Living Specimens

2012 ◽  
Vol 20 (4) ◽  
pp. 12-16
Author(s):  
Tilman Franke ◽  
Sebastian Rhode
Keyword(s):  
Three Dimensional ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Time Resolved ◽  
3D Images ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Deep Tissue Imaging ◽  
And Function ◽  
Structural Contrast

Two-photon microscopy (2PM) provides three-dimensional (3D) and four-dimensional (4D) (x, y, z, t) imaging in living specimens or under experimental physiological conditions very close to live. In conjunction with fluorescent labels, 2PM provides a powerful means of investigating the relationships between structure and function at the microscopic level that are key to understanding biological systems. This technique is able to provide time-resolved, 3D images of dynamic systems with near-diffraction-limited resolution and highly specific structural contrast.

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