Light-sheet functional imaging in fictively behaving zebrafish

2014 ◽  
Vol 11 (9) ◽  
pp. 883-884 ◽  
Author(s):  
Nikita Vladimirov ◽  
Yu Mu ◽  
Takashi Kawashima ◽  
Davis V Bennett ◽  
Chao-Tsung Yang ◽  
...  
Keyword(s):  
Functional Imaging ◽  
Light Sheet

Light sheet microscopy for fast functional imaging of 3D neuronal cultures in hydrogels

2020 ◽  
Author(s):  
Gustavo Castro-Olvera ◽  
Jorge Madrid-Wolff ◽  
Omar E. Olarte ◽  
Estefanía Estévez-Priego ◽  
Adriaan A. Ludl ◽  
...  
Keyword(s):  
Functional Imaging ◽  
Light Sheet ◽  
Neuronal Cultures ◽  
Light Sheet Microscopy

scholarly journals Whole-brain calcium imaging during physiological vestibular stimulation in larval zebrafish

2018 ◽  
Author(s):  
Geoffrey Migault ◽  
Thomas Panier ◽  
Raphaël Candelier ◽  
Georges Debrégeas ◽  
Volker Bormuth
Keyword(s):  
Virtual Environments ◽  
Functional Imaging ◽  
Light Sheet ◽  
Vestibular Stimulation ◽  
Brain Response ◽  
Whole Brain ◽  
Larval Zebrafish ◽  
In Vivo Functional Imaging ◽  
First Time

AbstractDuring in vivo functional imaging, animals are head-fixed and thus deprived from vestibular inputs, which severely hampers the design of naturalistic virtual environments. To overcome this limitation, we developed a miniaturized ultra-stable light-sheet microscope that can be dynamically rotated during imaging along with a head-restrained zebrafish larva. We demonstrate that this system enables whole-brain functional imaging at single-cell resolution under controlled vestibular stimulation. We recorded for the first time the dynamic whole-brain response of a vertebrate to physiological vestibular stimulation. This development largely expands the potential of virtual-reality systems to explore complex multisensory-motor integration in 3D.

Compact, easy-to-use adaptive optics module for light-sheet structural and functional imaging of neuronal structures

2021 ◽  
Author(s):  
Fabrice Harms ◽  
Antoine Hubert ◽  
Pauline Treimany ◽  
Cynthia Veilly ◽  
Guillaume Dovillaire ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Functional Imaging ◽  
Light Sheet

Increasing sensitivity and accuracy of brain-wide quantitative studies in light-sheet microscopy

2017 ◽  
Author(s):  
Caroline M. Müllenbroich ◽  
Ludovico Silvestri ◽  
Lapo Turrini ◽  
Tommaso Alterini ◽  
Antonino P. Di Giovanna ◽  
...  
Keyword(s):  
Functional Imaging ◽  
Fold Increase ◽  
Structural Data ◽  
Light Sheet ◽  
High Fidelity ◽  
Random Errors ◽  
Bessel Beams ◽  
Light Sheet Microscopy ◽  
Quantitative Studies ◽  
Increase In Accuracy

AbstractLight-sheet microscopy (LSM) has proven a useful tool in neuroscience to image whole brains with high frame rates at cellular resolution. LSM is employed either in combination with tissue clearing to reconstruct the cyto-architecture over the entire mouse brain or with intrinsically transparent samples like zebrafish larvae for functional imaging. Inherently to LSM, however, residual opaque objects cause stripe artifacts, which obscure features of interest and, during functional imaging, modulate fluorescence variations related to neuronal activity. Here, we report how Bessel beams reduce streaking artifacts and produce high-fidelity structural data. Furthermore, using Bessel beams, we demonstrate a fivefold increase in sensitivity to calcium transients and a 20 fold increase in accuracy in the detection of activity correlations in functional imaging. Our results demonstrate the contamination of data by systematic and random errors through Gaussian illumination and furthermore quantify the increase in fidelity of such data when using Bessel beams.

Whole-brain functional imaging with two-photon light-sheet microscopy

2015 ◽  
Vol 12 (5) ◽  
pp. 379-380 ◽  
Author(s):  
Sébastien Wolf ◽  
Willy Supatto ◽  
Georges Debrégeas ◽  
Pierre Mahou ◽  
Sergei G Kruglik ◽  
...  
Keyword(s):  
Functional Imaging ◽  
Light Sheet ◽  
Whole Brain ◽  
Two Photon ◽  
Light Sheet Microscopy

Functional Imaging with Light-Sheet Microscopy

2020 ◽  
pp. 21-54
Author(s):  
Raghav K. Chhetri ◽  
Philipp J. Keller
Keyword(s):  
Functional Imaging ◽  
Light Sheet ◽  
Light Sheet Microscopy

scholarly journals Two-photon light-sheet microscopy for high-speed whole-brain functional imaging of zebrafish neuronal physiology and pathology

2020 ◽  
Author(s):  
Giuseppe de Vito ◽  
Lapo Turrini ◽  
Chiara Fornetto ◽  
Pietro Ricci ◽  
Caroline Müllenbroich ◽  
...  
Keyword(s):  
Functional Imaging ◽  
High Speed ◽  
Light Sheet ◽  
Whole Brain ◽  
Two Photon ◽  
Light Sheet Microscopy ◽  
Neuronal Physiology

Whole-brain functional imaging at cellular resolution using light-sheet microscopy

2013 ◽  
Vol 10 (5) ◽  
pp. 413-420 ◽  
Author(s):  
Misha B Ahrens ◽  
Michael B Orger ◽  
Drew N Robson ◽  
Jennifer M Li ◽  
Philipp J Keller
Keyword(s):  
Functional Imaging ◽  
Light Sheet ◽  
Whole Brain ◽  
Light Sheet Microscopy ◽  
Cellular Resolution
Sign in / Sign up

Export Citation Format

Share Document