Gene therapy by skeletal muscle expression of decorin prevents fibrotic disease in rat kidney

1996 ◽  
Vol 2 (4) ◽  
pp. 418-423 ◽  
Author(s):  
Yoshitaka Isaka ◽  
Douglas K. Brees ◽  
Kazuko Ikegaya ◽  
Yasufumi Kaneda ◽  
Enyu Imai ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Gene Therapy ◽  
Rat Kidney ◽  
Fibrotic Disease

scholarly journals Successful gene therapy following direct injection of naked DNA encoding secreted protein into skeletal muscle

1996 ◽  
Vol 27 (2) ◽  
pp. 316
Author(s):  
Yukio Tsurumi ◽  
Satoshi Takeshita ◽  
Jonathan Passari ◽  
Marianne Kearney ◽  
Jeffrey R. Horowitz ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Gene Therapy ◽  
Direct Injection ◽  
Secreted Protein ◽  
Dna Encoding ◽  
Naked Dna

The mitotic clock in skeletal muscle regeneration, disease and cell mediated gene therapy

2005 ◽  
Vol 184 (1) ◽  
pp. 3-15 ◽  
Author(s):  
V. Mouly ◽  
A. Aamiri ◽  
A. Bigot ◽  
R. N. Cooper ◽  
S. Di Donna ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Gene Therapy ◽  
Muscle Regeneration ◽  
Skeletal Muscle Regeneration ◽  
Mitotic Clock

Gene Therapy of Skeletal Muscle Disorders Using Viral Vectors

Muscle ◽  
2012 ◽  
pp. 1045-1051 ◽  
Author(s):  
Andrea L.H. Arnett ◽  
Julian N. Ramos ◽  
Jeffrey S. Chamberlain
Keyword(s):  
Skeletal Muscle ◽  
Gene Therapy ◽  
Viral Vectors ◽  
Muscle Disorders ◽  
Skeletal Muscle Disorders

α-AMINO-n-BUTYRIC ACID IN TRANSAMINATION

1959 ◽  
Vol 37 (4) ◽  
pp. 599-604
Author(s):  
G. Y. N. Iyer ◽  
M. Sukumaran
Keyword(s):  
Skeletal Muscle ◽  
Butyric Acid ◽  
Direct Evidence ◽  
Rat Kidney ◽  
Transaminase Activity ◽  
Transamination Reaction ◽  
Kidney Liver

The transamination reaction between α-amino-n-butyric acid and α-keto-glutarate or pyruvate or oxaloacetate in the presence of homogenates of rat kidney, liver, heart, and skeletal muscle has been studied. Direct evidence is presented for transamination with α-ketoglutarate in the presence of the first three tissues and with pyruvate in the presence of kidney and liver. Appreciable amounts of alanine are formed in the course of transamination with oxaloacetate. Of the four tissues the liver appears to be quantitatively the most important by virtue of its mass and relatively high specific transaminase activity.

Autoimmune anemia in macaques following erythropoietin gene therapy

Blood ◽  
2004 ◽  
Vol 103 (9) ◽  
pp. 3303-3304 ◽  
Author(s):  
Pierre Chenuaud ◽  
Thibaut Larcher ◽  
Joseph E. Rabinowitz ◽  
Nathalie Provost ◽  
Yan Cherel ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Gene Therapy ◽  
Autoimmune Disease ◽  
Neutralizing Antibodies ◽  
Adeno Associated Virus ◽  
Endogenous Erythropoietin ◽  
Cynomolgus Macaques ◽  
Vector Sequences ◽  
Gene Expressing ◽  
Self Antigen

Abstract We delivered the homologous erythropoietin (Epo) cDNA driven from a doxycycline-regulated promoter via recombinant adeno-associated virus in skeletal muscle of 9 cynomolgus macaques. Upon induction, rapid supraphysiologic levels of Epo were obtained. Unexpectedly, some individuals developed a profound anemia that correlated with the appearance of neutralizing antibodies against the endogenous Epo. Both the endogenous erythropoietin and vector sequences were identical. This is the first example of the inadvertent development of an autoimmune disease in primates as a result of gene transfer of a gene expressing a self-antigen. It raises some concerns when a therapeutic protein is produced at high levels from an ectopic site. (Blood. 2004;103:3303-3304)

scholarly journals Isolation of functional mitochondria from rat kidney and skeletal muscle without manual homogenization

2011 ◽  
Vol 418 (2) ◽  
pp. 213-223 ◽  
Author(s):  
Vera S. Gross ◽  
Heather K. Greenberg ◽  
Sergei V. Baranov ◽  
Greta M. Carlson ◽  
Irina G. Stavrovskaya ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Rat Kidney

scholarly journals MEMBRANE JUNCTIONS IN THE INTERMEMBRANE SPACE OF MITOCHONDRIA FROM MAMMALIAN TISSUES

1974 ◽  
Vol 60 (3) ◽  
pp. 653-663 ◽  
Author(s):  
Akitsugu Saito ◽  
Murray Smigel ◽  
Sidney Fleischer
Keyword(s):  
Skeletal Muscle ◽  
Cross Sections ◽  
Rat Kidney ◽  
Respiratory Control ◽  
Heart Tissue ◽  
Intermembrane Space ◽  
Mitochondrial Membranes ◽  
Fresh Tissue ◽  
Isolated Mitochondria ◽  
Mammalian Tissues

There have been several reports describing paracrystalline arrays in the intermembrane space of mitochondria. On closer inspection these structures appear to be junctions of two adjoining membranes. There are two types. They can be formed between the outer and inner mitochondrial membranes (designated outer-inner membrane junctions) or between two cristal membranes (intercristal membrane junctions). In rat heart, adjoining membranes appeared associated via a central dense midline approximately 30 Å wide. In rat kidney, the junction had a ladder-like appearance with electron-dense "bridges" approximately 80 Å wide, spaced 130 Å apart, connecting the adjoining membranes. We have investigated the conditions which favor the visualization of such structures in mitochondria. Heart mitochondria isolated rapidly from fresh tissue (within 30 min of death) contain membrane junctions in approximately 10–15% of the cross sections. This would indicate that the percentage of membrane junctions in the entire mitochondrion is far greater. Mitochondria isolated from heart tissue which was stored for 1 h at 0°–4°C showed an increased number of membrane junctions, so that 80% of the mitochondrial cross sections show membrane junctions. No membrane junctions are observed in mitochondria in rapidly fixed fresh tissue or in mitochondria isolated from tissue disrupted in fixative. Thus, the visualization of junctions in the intermembrane space of mitochondria appears to be dependent upon the storage of tissue after death. Membrane junctions can also be observed in mitochondria from other stored tissues such as skeletal muscle, kidney, and interstitial cells from large and small intestine. In each case, no such junctions are observed in these tissues when they are fixed immediately after removal from the animal. It would appear that most studies in the literature in which isolated mitochondria from tissues such as heart or kidney were used were carried out on mitochondria which contained membrane junctions. The presence of such structures does not significantly affect normal mitochondrial function in terms of respiratory control and oxidative phosphorylation.

scholarly journals NEPHROTOXIC SERUM NEPHRITIS IN RATS

1956 ◽  
Vol 104 (4) ◽  
pp. 487-499 ◽  
Author(s):  
Howard C. Goodman ◽  
James H. Baxter
Keyword(s):  
Skeletal Muscle ◽  
Ammonium Sulfate ◽  
Protective Factor ◽  
Rat Kidney ◽  
Tryptic Digestion ◽  
Tissue Antigen ◽  
Rat Lung ◽  
High Concentration ◽  
Kidney Homogenate ◽  
Rat Tissue

A soluble protective factor, capable of absorbing nephrotoxic antibodies from anti-rat kidney serum, can be obtained in high concentration by tryptic digestion of rat kidney homogenate. The factor is no longer antigenic or at most only slightly so. It is stable at 60°, destroyed by boiling, is non-dialyzable, can be precipitated by ammonium sulfate, but resists destruction by proteolytic and other enzymes. In accord with previous studies on the organ localization of the rat tissue antigen responsible for the production of nephrotoxic antibodies, the soluble protective factor, or antigen derivative, can be obtained by tryptic digestion of rat lung, skeletal muscle, heart, and liver.

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