scholarly journals The K526R substitution in viral protein PB2 enhances the effects of E627K on influenza virus replication

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Wenjun Song ◽  
Pui Wang ◽  
Bobo Wing-Yee Mok ◽  
Siu-Ying Lau ◽  
Xiaofeng Huang ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Virus Replication ◽  
Viral Protein ◽  
Influenza Virus Replication

scholarly journals Effects of Traditional Kampo Drugs and Their Constituent Crude Drugs on Influenza Virus Replication In Vitro: Suppression of Viral Protein Synthesis by Glycyrrhizae Radix

2019 ◽  
Vol 2019 ◽  
pp. 1-12
Author(s):  
Toshihito Nomura ◽  
Masaya Fukushi ◽  
Kosuke Oda ◽  
Akifumi Higashiura ◽  
Takashi Irie ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Influenza Virus ◽  
Virus Replication ◽  
Viral Protein ◽  
Culture Media ◽  
Virus Infectivity ◽  
Viral Protein Synthesis ◽  
Glycyrrhizae Radix ◽  
Influenza Virus Replication ◽  
Crude Drugs

An influenza virus epidemic is an important issue in public hygiene, and continuous development on an effective drug is required. Kampo medicine is a traditional medicine that is used clinically for treatment of various diseases in Japan and other East Asian countries. We evaluated the effects of the Kampo drugs maoto, kakkonto, senkyuchachosan, jinkokato, and bakumondoto, which are prescribed for treatment of respiratory symptoms including symptoms caused by influenza, on influenza virus replication in cultured cells. Culture media of influenza virus-infected MDCK(+) cells were tested for hemagglutination and infectivity at 24 h after the addition of Kampo drugs at various concentrations, and four of the five Kampo drugs were found to inhibit virus release to the culture media. These drugs inactivated virus infectivity not by acting on virus particles but by acting on virus-infected cells. In addition, when six crude drugs (Atractylodis lanceae rhizome, Citri unshiu pericarpium, Cnidii rhizome, Glycyrrhizae radix, Rehmanniae radix, and Saposhnikoviae radix) that constitute the effective Kampo drugs were examined, the strongest activity was found for Glycyrrhizae radix (IC50 = 0.27 mg/ml), which selectively suppressed viral protein synthesis. Since Glycyrrhizae radix is contained in many Kampo drugs, it may give anti-influenza virus activity to a broad range of Kampo drugs.

scholarly journals Targeting Cell Division Cycle 25 Homolog B To Regulate Influenza Virus Replication

2013 ◽  
Vol 87 (24) ◽  
pp. 13775-13784 ◽  
Author(s):  
O. Perwitasari ◽  
A. C. Torrecilhas ◽  
X. Yan ◽  
S. Johnson ◽  
C. White ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Cell Division ◽  
Virus Replication ◽  
Cell Division Cycle ◽  
Influenza Virus Replication

Inhibition of influenza virus replication by adlay tea

2017 ◽  
Vol 98 (5) ◽  
pp. 1899-1905 ◽  
Author(s):  
Emiko Nagai ◽  
Miwa Iwai ◽  
Ritsuko Koketsu ◽  
Riho Sogabe ◽  
Ryosuke Morimoto ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Virus Replication ◽  
Influenza Virus Replication

scholarly journals Network-Guided Discovery of Influenza Virus Replication Host Factors

mBio ◽  
2018 ◽  
Vol 9 (6) ◽  
Author(s):  
Emily E. Ackerman ◽  
Eiryo Kawakami ◽  
Manami Katoh ◽  
Tokiko Watanabe ◽  
Shinji Watanabe ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Viral Replication ◽  
Virus Replication ◽  
Drug Targets ◽  
Antiviral Drug ◽  
Viral Proteins ◽  
Host Factors ◽  
Ppi Network ◽  
Host Proteins ◽  
Influenza Virus Replication

ABSTRACTThe positions of host factors required for viral replication within a human protein-protein interaction (PPI) network can be exploited to identify drug targets that are robust to drug-mediated selective pressure. Host factors can physically interact with viral proteins, be a component of virus-regulated pathways (where proteins do not interact with viral proteins), or be required for viral replication but unregulated by viruses. Here, we demonstrate a method of combining human PPI networks with virus-host PPI data to improve antiviral drug discovery for influenza viruses by identifying target host proteins. Analysis shows that influenza virus proteins physically interact with host proteins in network positions significant for information flow, even after the removal of known abundance-degree bias within PPI data. We have isolated a subnetwork of the human PPI network that connects virus-interacting host proteins to host factors that are important for influenza virus replication without physically interacting with viral proteins. The subnetwork is enriched for signaling and immune processes distinct from those associated with virus-interacting proteins. Selecting proteins based on subnetwork topology, we performed an siRNA screen to determine whether the subnetwork was enriched for virus replication host factors and whether network position within the subnetwork offers an advantage in prioritization of drug targets to control influenza virus replication. We found that the subnetwork is highly enriched for target host proteins—more so than the set of host factors that physically interact with viral proteins. Our findings demonstrate that network positions are a powerful predictor to guide antiviral drug candidate prioritization.IMPORTANCEIntegrating virus-host interactions with host protein-protein interactions, we have created a method using these established network practices to identify host factors (i.e., proteins) that are likely candidates for antiviral drug targeting. We demonstrate that interaction cascades between host proteins that directly interact with viral proteins and host factors that are important to influenza virus replication are enriched for signaling and immune processes. Additionally, we show that host proteins that interact with viral proteins are in network locations of power. Finally, we demonstrate a new network methodology to predict novel host factors and validate predictions with an siRNA screen. Our results show that integrating virus-host proteins interactions is useful in the identification of antiviral drug target candidates.

Inhibitory effect of hirsutine on influenza virus replication in vitro

1997 ◽  
Vol 34 (2) ◽  
pp. A88 ◽  
Author(s):  
K. Konno ◽  
H. Inoue ◽  
M. Fujiwara ◽  
T. Mizuta ◽  
H. Takayama ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Virus Replication ◽  
Inhibitory Effect ◽  
Influenza Virus Replication
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