Site-specific gene targeting in mouse embryonic stem cells with intact bacterial artificial chromosomes

2003 ◽  
Vol 21 (4) ◽  
pp. 447-451 ◽  
Author(s):  
Yi Yang ◽  
Brian Seed
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Targeting ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Specific Gene ◽  
Bacterial Artificial Chromosomes ◽  
Site Specific ◽  
Artificial Chromosomes

scholarly journals Targeting frequency for deletion vectors in embryonic stem cells.

1994 ◽  
Vol 14 (4) ◽  
pp. 2404-2410 ◽  
Author(s):  
H Zhang ◽  
P Hasty ◽  
A Bradley
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Targeting ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Hypoxanthine Phosphoribosyltransferase ◽  
Genomic Deletions ◽  
Wide Range

We analyzed the gene targeting frequencies and recombination products generated by a series of replacement deletion vectors which target the hprt (hypoxanthine phosphoribosyltransferase) locus in mouse embryonic stem cells. We found that the targeting frequency of a 19.2-kb deletion was comparable to that of a 3-kb deletion or a conventional replacement event in which a 1.7-kb fragment was inserted into the locus. We also observed different integration patterns for these deletion vectors. A result of this finding is that a wide range of genomic deletions in embryonic stem cells is feasible.

scholarly journals The length of homology required for gene targeting in embryonic stem cells

1991 ◽  
Vol 11 (11) ◽  
pp. 5586-5591 ◽  
Author(s):  
P Hasty ◽  
J Rivera-Pérez ◽  
A Bradley
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Homologous Recombination ◽  
Gene Targeting ◽  
Es Cells ◽  
Embryonic Stem ◽  
Critical Length ◽  
Gene Replacement ◽  
Site Specific ◽  
The Relationship

Homologous recombination has been used to introduce site-specific mutations into murine embryonic stem (ES) cells with both insertion and replacement vectors. In this study, we compared the frequency of gene targeting with various lengths of homology and found a dramatic increase in targeting with an increase in homology from 1.3 to 6.8 kb. We examined in detail the relationship between the length of homology and the gene-targeting frequency for replacement vectors and found that a critical length of homology is needed for targeting. Adding greater lengths of homology to this critical length has less of an effect on the targeting frequency. We also analyzed the lengths of homology necessary on both arms of the vector for gene replacement events and found that 472 bp of homology is used as efficiently as 1.2 kb in the formation and resolution of crossover junctions.

scholarly journals Bi-allelic gene targeting in mouse embryonic stem cells

Methods ◽  
2011 ◽  
Vol 53 (4) ◽  
pp. 331-338 ◽  
Author(s):  
Peri H. Tate ◽  
William C. Skarnes
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Targeting ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Allelic Gene

scholarly journals Optimized Vector for Conditional Gene Targeting in Mouse Embryonic Stem Cells

BioTechniques ◽  
2003 ◽  
Vol 34 (6) ◽  
pp. 1136-1140 ◽  
Author(s):  
Marcus Conrad ◽  
Markus Brielmeier ◽  
Wolfgang Wurst ◽  
Georg W. Bornkamm
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Targeting ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Conditional Gene Targeting
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