scholarly journals Identification of Stage-Specific Gene Expression Signatures in Response to Retinoic Acid during the Neural Differentiation of Mouse Embryonic Stem Cells

2012 ◽  
Vol 3 ◽  
Author(s):  
Hiromi Akanuma ◽  
Xian-Yang Qin ◽  
Reiko Nagano ◽  
Tin-Tin Win-Shwe ◽  
Satoshi Imanishi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Retinoic Acid ◽  
Embryonic Stem Cells ◽  
Neural Differentiation ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Specific Gene ◽  
Specific Gene Expression ◽  
Gene Expression Signatures

Alteration of gene expression profile in mouse embryonic stem cells and neural differentiation deficits by ethephon

2020 ◽  
Vol 39 (11) ◽  
pp. 1518-1527
Author(s):  
S Mohammadi Nejad ◽  
M Hodjat ◽  
SA Mousavi ◽  
M Baeeri ◽  
MA Rezvanfar ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Neural Differentiation ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Differentiation Potential

Ethephon, a member of the organophosphorus compounds, is one of the most widely used plant growth regulators for artificial ripening. Although million pounds of this chemical is being used annually, the knowledge regarding its molecular toxicity is yet not sufficient. The purpose of this study was to evaluate the potential developmental toxicity of ethephon using embryonic stem cell model. The mouse embryonic stem cells (mESCs) were exposed to various concentrations of ethephon and the viability, cell cycle alteration and changes in the gene expression profile were evaluated using high-throughput RNA sequencing. Further, the effect of ethephon on neural differentiation potential was examined. The results showed that ethephon at noncytotoxic doses induced cell cycle arrest in mESCs. Gene ontology enrichment analysis showed that terms related to cell fate and organismal development, including neuron fate commitment, embryo development and cardiac cell differentiation, were markedly enriched in ethephon-treated cells. Neural induction of mESCs in the presence of ethephon was inhibited and the expression of neural genes was decreased in differentiated cells. Results obtained from this work clearly demonstrate that ethephon affects the gene expression profile of undifferentiated mESCs and prevents neural differentiation. Therefore, more caution against the frequent application of ethephon is advised.

scholarly journals The ciliary proteins Meckelin and Jouberin are required for retinoic acid-dependent neural differentiation of mouse embryonic stem cells

2014 ◽  
Vol 87 (3-4) ◽  
pp. 134-146 ◽  
Author(s):  
Sveva Romani ◽  
Barbara Illi ◽  
Roberta De Mori ◽  
Mauro Savino ◽  
Joseph G. Gleeson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Embryonic Stem Cells ◽  
Neural Differentiation ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells

Interplay of leukemia inhibitory factor and retinoic acid on neural differentiation of mouse embryonic stem cells

2007 ◽  
Vol 85 (12) ◽  
pp. 2686-2701 ◽  
Author(s):  
Raquel Martín-Ibáñez ◽  
Noelia Urbán ◽  
Solène Sergent-Tanguy ◽  
José Ramón Pineda ◽  
Núria Garrido-Clua ◽  
...  
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Embryonic Stem Cells ◽  
Leukemia Inhibitory Factor ◽  
Neural Differentiation ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Inhibitory Factor
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