scholarly journals Nanoscale imaging of clinical specimens using pathology-optimized expansion microscopy

2017 ◽  
Vol 35 (8) ◽  
pp. 757-764 ◽  
Author(s):  
Yongxin Zhao ◽  
Octavian Bucur ◽  
Humayun Irshad ◽  
Fei Chen ◽  
Astrid Weins ◽  
...  
Keyword(s):  
Clinical Specimens ◽  
Nanoscale Imaging

scholarly journals Expansion Pathology: Nanoscale Imaging of Clinical Specimens with Optical Microscopy

2021 ◽  
Vol 27 (S1) ◽  
pp. 1902-1903
Author(s):  
Yongxin Zhao ◽  
Feifei Fu ◽  
Octavian Bucur
Keyword(s):  
Optical Microscopy ◽  
Clinical Specimens ◽  
Nanoscale Imaging

scholarly journals Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathology

2020 ◽  
Vol 15 (5) ◽  
pp. 1649-1672 ◽  
Author(s):  
Octavian Bucur ◽  
Feifei Fu ◽  
Mike Calderon ◽  
Geetha H. Mylvaganam ◽  
Ngoc L. Ly ◽  
...  
Keyword(s):  
Rapid Expansion ◽  
Clinical Specimens ◽  
Nanoscale Imaging

A combined pseudoreplica-immunocytochemical technique for research and diagnostic virology

1990 ◽  
Vol 48 (3) ◽  
pp. 900-901
Author(s):  
Blayne Fritz ◽  
Stanley J. Naides ◽  
Kenneth Moore
Keyword(s):  
Phosphotungstic Acid ◽  
Immunogold Labelling ◽  
Uranyl Acetate ◽  
Distilled Water ◽  
Clinical Specimens ◽  
Tissue Sections ◽  
Specimen Retrieval ◽  
Transmission Electron ◽  
Viral Particles ◽  
Diagnostic Virology

The pseudoreplica method of staining viral particles for visualization by transmission electron microscopy is a very popular technique. The ability to concentrate clinical specimens while semi-embedding viral particles makes it especially well suited for morphologic and diagnostic virology. Immunolabelling viral particles with colloidal gold is a technique frequently employed by both research and diagnostic virologists. We have characterized a procedure which provides the advantage of both by modifying and combining pseudoreplica staining and immunogold labelling.Modification of specimen retrieval and delay of staining allows us to utilize pseudoreplica processed specimens within our standard immunogold labelling protocol. In brief, we absorbed samples onto 2% agarose, added.25% Formvar and wicked dry. We then floated the Formvar-virus film onto double distilled water, added grids and retrieved with parafilm. The Formvar-virus specimens were then treated as thin tissue sections within our standard two stage immunolabelling protocol. Following completion of immunogold labelling; each grid was negatively stained with phosphotungstic acid or uranyl acetate contrast stains.

Prevalence of Vancomycin Resistant Enterococci in Various Clinical Specimens in Tertiary Care Hospital in North Delhi

2012 ◽  
Vol 3 (3) ◽  
pp. 141-144
Author(s):  
Swati Chaudhary ◽  
◽  
Swastika Aggarwal ◽  
Pawan Kumar ◽  
SK Aggarwal SK Aggarwal ◽  
...  
Keyword(s):  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Care Hospital ◽  
Clinical Specimens ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant

Detection of Genes Encoding of Extended-Spectrum and AmpC β-Lactamases in Klebsiella pneumoniae Isolates from Clinical Specimens

2015 ◽  
Vol 18 (2) ◽  
pp. 125-132
Author(s):  
Ghusoon A. Abdulhasan ◽  
◽  
Hula Y. Fadhil ◽  
Kifah A. Jasem ◽  
◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Clinical Specimens ◽  
Extended Spectrum ◽  
Genes Encoding

scholarly journals In situ nanoscale imaging of moiré superlattices in twisted van der Waals heterostructures

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Yue Luo ◽  
Rebecca Engelke ◽  
Marios Mattheakis ◽  
Michele Tamagnone ◽  
Stephen Carr ◽  
...  
Keyword(s):  
Van Der Waals ◽  
Van Der Waals Heterostructures ◽  
Nanoscale Imaging
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