Tumour-specific proline vulnerability uncovered by differential ribosome codon reading

Nature ◽  
2016 ◽  
Vol 530 (7591) ◽  
pp. 490-494 ◽  
Author(s):  
Fabricio Loayza-Puch ◽  
Koos Rooijers ◽  
Levi C. M. Buil ◽  
Jelle Zijlstra ◽  
Joachim F. Oude Vrielink ◽  
...  
Keyword(s):  
Codon Reading

scholarly journals Aberrations of the classic codon reading scheme during protein synthesis in vitro.

1980 ◽  
Vol 255 (10) ◽  
pp. 4583-4588 ◽  
Author(s):  
T. Samuelsson ◽  
P. Elias ◽  
F. Lustig ◽  
T. Axberg ◽  
G. Fölsch ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Codon Reading

scholarly journals Codon reading and translational error. Reading of the glutamine and lysine codons during protein synthesis in vitro.

1981 ◽  
Vol 256 (6) ◽  
pp. 2635-2643 ◽  
Author(s):  
F. Lustig ◽  
P. Elias ◽  
T. Axberg ◽  
T. Samuelsson ◽  
I. Tittawella ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Codon Reading

scholarly journals N 6-Methyladenosines in mRNAs reduce the accuracy of codon reading by transfer RNAs and peptide release factors

2021 ◽  
Vol 49 (5) ◽  
pp. 2684-2699
Author(s):  
Ka-Weng Ieong ◽  
Gabriele Indrisiunaite ◽  
Arjun Prabhakar ◽  
Joseph D Puglisi ◽  
Måns Ehrenberg
Keyword(s):  
Single Molecule ◽  
Stop Codon ◽  
Product Formation ◽  
Release Factors ◽  
Substrate Complex ◽  
Peptidyl Transfer ◽  
Enzyme Substrate ◽  
Peptide Release ◽  
Accuracy Ratio ◽  
Codon Reading

Abstract We used quench flow to study how N6-methylated adenosines (m6A) affect the accuracy ratio between kcat/Km (i.e. association rate constant (ka) times probability (Pp) of product formation after enzyme-substrate complex formation) for cognate and near-cognate substrate for mRNA reading by tRNAs and peptide release factors 1 and 2 (RFs) during translation with purified Escherichia coli components. We estimated kcat/Km for Glu-tRNAGlu, EF-Tu and GTP forming ternary complex (T3) reading cognate (GAA and Gm6AA) or near-cognate (GAU and Gm6AU) codons. ka decreased 10-fold by m6A introduction in cognate and near-cognate cases alike, while Pp for peptidyl transfer remained unaltered in cognate but increased 10-fold in near-cognate case leading to 10-fold amino acid substitution error increase. We estimated kcat/Km for ester bond hydrolysis of P-site bound peptidyl-tRNA by RF2 reading cognate (UAA and Um6AA) and near-cognate (UAG and Um6AG) stop codons to decrease 6-fold or 3-fold by m6A introduction, respectively. This 6-fold effect on UAA reading was also observed in a single-molecule termination assay. Thus, m6A reduces both sense and stop codon reading accuracy by decreasing cognate significantly more than near-cognate kcat/Km, in contrast to most error inducing agents and mutations, which increase near-cognate at unaltered cognate kcat/Km.

“Two out of three” codon reading leading to mistranslation in vivo

1980 ◽  
Vol 177 (3) ◽  
pp. 439-445 ◽  
Author(s):  
Jack Parker ◽  
James D. Friesen
Keyword(s):  
Codon Reading

Unorthodox codon reading and the evolution of the genetic code

Cell ◽  
1981 ◽  
Vol 23 (2) ◽  
pp. 305-306 ◽  
Author(s):  
Ulf Lagerkvist
Keyword(s):  
Genetic Code ◽  
Codon Reading

scholarly journals Unconventional codon reading by Mycoplasma mycoides tRNAs as revealed by partial sequence analysis

1989 ◽  
Vol 258 (3) ◽  
pp. 869-873 ◽  
Author(s):  
Y S Guindy ◽  
T Samuelsson ◽  
T I Johansen
Keyword(s):  
Codon Position ◽  
Living Organism ◽  
Trna Genes ◽  
Gene Products ◽  
Free Living ◽  
The Third ◽  
The Family ◽  
Mycoplasma Mycoides ◽  
Codon Reading ◽  
Unconventional Method

Continuing our investigation of the tRNA genes and gene products in Mycoplasma mycoides, we report the sequence of the gene for tRNALeu (CAA) as well as partial primary structures of the following tRNAs: Leu (CAA), Leu (UAG), Arg (UCU), Thr (AGU) and Ile (CAU). It is suggested that in M. mycoides, at least some of the family codon boxes are read by only one tRNA each, using an unconventional method which does not discriminate between the nucleotides in the third codon position. M. mycoides is the first free-living organism known to use an unconventional method of this kind.

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