scholarly journals mTORC1 in the Paneth cell niche couples intestinal stem-cell function to calorie intake

Nature ◽  
2012 ◽  
Vol 486 (7404) ◽  
pp. 490-495 ◽  
Author(s):  
Ömer H. Yilmaz ◽  
Pekka Katajisto ◽  
Dudley W. Lamming ◽  
Yetis Gültekin ◽  
Khristian E. Bauer-Rowe ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Function ◽  
Paneth Cell ◽  
Intestinal Stem Cell ◽  
Calorie Intake ◽  
Cell Niche

scholarly journals Regulation of the Paneth cell niche by exogenous L ‐arginine couples the intestinal stem cell function

2020 ◽  
Vol 34 (8) ◽  
pp. 10299-10315
Author(s):  
Qihang Hou ◽  
Yuanyang Dong ◽  
Qinghua Yu ◽  
Bo Wang ◽  
Shen Le ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Function ◽  
Paneth Cell ◽  
Intestinal Stem Cell ◽  
Cell Niche

Rab8a vesicles regulate Wnt ligand delivery and Paneth cell maturation at the intestinal stem cell niche

2015 ◽  
Vol 128 (13) ◽  
pp. e1.2-e1.2
Author(s):  
S. Das ◽  
S. Yu ◽  
R. Sakamori ◽  
P. Vedula ◽  
Q. Feng ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Niche ◽  
Paneth Cell ◽  
Cell Maturation ◽  
Intestinal Stem Cell ◽  
Cell Niche

scholarly journals Intestinal Stem Cell Niche Insights Gathered from Both In Vivo and Novel In Vitro Models

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Nikolce Gjorevski ◽  
Paloma Ordóñez-Morán
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Stem Cell Niche ◽  
Cell Function ◽  
Complex Structure ◽  
Intestinal Stem Cell ◽  
Intestinal Damage ◽  
Cell Niche

Intestinal stem cells are located at the base of the crypts and are surrounded by a complex structure called niche. This environment is composed mainly of epithelial cells and stroma which provides signals that govern cell maintenance, proliferation, and differentiation. Understanding how the niche regulates stem cell fate by controlling developmental signaling pathways will help us to define how stem cells choose between self-renewal and differentiation and how they maintain their undifferentiated state. Tractable in vitro assay systems, which reflect the complexity of the in vivo situation but provide higher level of control, would likely be crucial in identifying new players and mechanisms controlling stem cell function. Knowledge of the intestinal stem cell niche gathered from both in vivo and novel in vitro models may help us improve therapies for tumorigenesis and intestinal damage and make autologous intestinal transplants a feasible clinical practice.

scholarly journals HMGA1 amplifies Wnt signalling and expands the intestinal stem cell compartment and Paneth cell niche

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Lingling Xian ◽  
Dan Georgess ◽  
Tait Huso ◽  
Leslie Cope ◽  
Amy Belton ◽  
...  
Keyword(s):  
Stem Cell ◽  
Paneth Cell ◽  
Wnt Signalling ◽  
Intestinal Stem Cell ◽  
Cell Compartment ◽  
Stem Cell Compartment ◽  
Cell Niche

scholarly journals Paneth Cell-Rich Regions Separated by a Cluster of Lgr5+ Cells Initiate Crypt Fission in the Intestinal Stem Cell Niche

PLoS Biology ◽  
2016 ◽  
Vol 14 (6) ◽  
pp. e1002491 ◽  
Author(s):  
Alistair J. Langlands ◽  
Axel A. Almet ◽  
Paul L. Appleton ◽  
Ian P. Newton ◽  
James M. Osborne ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Niche ◽  
Paneth Cell ◽  
Intestinal Stem Cell ◽  
Crypt Fission ◽  
Cell Niche

scholarly journals PDGFRα+ pericryptal stromal cells are the critical source of Wnts and RSPO3 for murine intestinal stem cells in vivo

2018 ◽  
Vol 115 (14) ◽  
pp. E3173-E3181 ◽  
Author(s):  
Gediminas Greicius ◽  
Zahra Kabiri ◽  
Kristmundur Sigmundsson ◽  
Chao Liang ◽  
Ralph Bunte ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Niche ◽  
Ex Vivo ◽  
Paneth Cell ◽  
Intestinal Stem Cells ◽  
Intestinal Stem Cell ◽  
Intestinal Crypt ◽  
Proliferation And Differentiation ◽  
Cell Niche

Wnts and R-spondins (RSPOs) support intestinal homeostasis by regulating crypt cell proliferation and differentiation. Ex vivo, Wnts secreted by Paneth cells in organoids can regulate the proliferation and differentiation of Lgr5-expressing intestinal stem cells. However, in vivo, Paneth cell and indeed all epithelial Wnt production is completely dispensable, and the cellular source of Wnts and RSPOs that maintain the intestinal stem-cell niche is not known. Here we investigated both the source and the functional role of stromal Wnts and RSPO3 in regulation of intestinal homeostasis. RSPO3 is highly expressed in pericryptal myofibroblasts in the lamina propria and is several orders of magnitude more potent than RSPO1 in stimulating both Wnt/β-catenin signaling and organoid growth. Stromal Rspo3 ablation ex vivo resulted in markedly decreased organoid growth that was rescued by exogenous RSPO3 protein. Pdgf receptor alpha (PdgfRα) is known to be expressed in pericryptal myofibroblasts. We therefore evaluated if PdgfRα identified the key stromal niche cells. In vivo, Porcn excision in PdgfRα+ cells blocked intestinal crypt formation, demonstrating that Wnt production in the stroma is both necessary and sufficient to support the intestinal stem-cell niche. Mice with Rspo3 excision in the PdgfRα+ cells had decreased intestinal crypt Wnt/β-catenin signaling and Paneth cell differentiation and were hypersensitive when stressed with dextran sodium sulfate. The data support a model of the intestinal stem-cell niche regulated by both Wnts and RSPO3 supplied predominantly by stromal pericryptal myofibroblasts marked by PdgfRα.

scholarly journals Rab8a vesicles regulate Wnt ligand delivery and Paneth cell maturation at the intestinal stem cell niche

Development ◽  
2015 ◽  
Vol 142 (12) ◽  
pp. 2147-2162 ◽  
Author(s):  
S. Das ◽  
S. Yu ◽  
R. Sakamori ◽  
P. Vedula ◽  
Q. Feng ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Niche ◽  
Paneth Cell ◽  
Cell Maturation ◽  
Intestinal Stem Cell ◽  
Cell Niche
Sign in / Sign up

Export Citation Format

Share Document