scholarly journals Rab8a vesicles regulate Wnt ligand delivery and Paneth cell maturation at the intestinal stem cell niche

Development ◽  
2015 ◽  
Vol 142 (12) ◽  
pp. 2147-2162 ◽  
Author(s):  
S. Das ◽  
S. Yu ◽  
R. Sakamori ◽  
P. Vedula ◽  
Q. Feng ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Niche ◽  
Paneth Cell ◽  
Cell Maturation ◽  
Intestinal Stem Cell ◽  
Cell Niche

Rab8a vesicles regulate Wnt ligand delivery and Paneth cell maturation at the intestinal stem cell niche

2015 ◽  
Vol 128 (13) ◽  
pp. e1.2-e1.2
Author(s):  
S. Das ◽  
S. Yu ◽  
R. Sakamori ◽  
P. Vedula ◽  
Q. Feng ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Niche ◽  
Paneth Cell ◽  
Cell Maturation ◽  
Intestinal Stem Cell ◽  
Cell Niche

scholarly journals Paneth Cell-Rich Regions Separated by a Cluster of Lgr5+ Cells Initiate Crypt Fission in the Intestinal Stem Cell Niche

PLoS Biology ◽  
2016 ◽  
Vol 14 (6) ◽  
pp. e1002491 ◽  
Author(s):  
Alistair J. Langlands ◽  
Axel A. Almet ◽  
Paul L. Appleton ◽  
Ian P. Newton ◽  
James M. Osborne ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Niche ◽  
Paneth Cell ◽  
Intestinal Stem Cell ◽  
Crypt Fission ◽  
Cell Niche

scholarly journals PDGFRα+ pericryptal stromal cells are the critical source of Wnts and RSPO3 for murine intestinal stem cells in vivo

2018 ◽  
Vol 115 (14) ◽  
pp. E3173-E3181 ◽  
Author(s):  
Gediminas Greicius ◽  
Zahra Kabiri ◽  
Kristmundur Sigmundsson ◽  
Chao Liang ◽  
Ralph Bunte ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Niche ◽  
Ex Vivo ◽  
Paneth Cell ◽  
Intestinal Stem Cells ◽  
Intestinal Stem Cell ◽  
Intestinal Crypt ◽  
Proliferation And Differentiation ◽  
Cell Niche

Wnts and R-spondins (RSPOs) support intestinal homeostasis by regulating crypt cell proliferation and differentiation. Ex vivo, Wnts secreted by Paneth cells in organoids can regulate the proliferation and differentiation of Lgr5-expressing intestinal stem cells. However, in vivo, Paneth cell and indeed all epithelial Wnt production is completely dispensable, and the cellular source of Wnts and RSPOs that maintain the intestinal stem-cell niche is not known. Here we investigated both the source and the functional role of stromal Wnts and RSPO3 in regulation of intestinal homeostasis. RSPO3 is highly expressed in pericryptal myofibroblasts in the lamina propria and is several orders of magnitude more potent than RSPO1 in stimulating both Wnt/β-catenin signaling and organoid growth. Stromal Rspo3 ablation ex vivo resulted in markedly decreased organoid growth that was rescued by exogenous RSPO3 protein. Pdgf receptor alpha (PdgfRα) is known to be expressed in pericryptal myofibroblasts. We therefore evaluated if PdgfRα identified the key stromal niche cells. In vivo, Porcn excision in PdgfRα+ cells blocked intestinal crypt formation, demonstrating that Wnt production in the stroma is both necessary and sufficient to support the intestinal stem-cell niche. Mice with Rspo3 excision in the PdgfRα+ cells had decreased intestinal crypt Wnt/β-catenin signaling and Paneth cell differentiation and were hypersensitive when stressed with dextran sodium sulfate. The data support a model of the intestinal stem-cell niche regulated by both Wnts and RSPO3 supplied predominantly by stromal pericryptal myofibroblasts marked by PdgfRα.

Tu1731 Survivin Controls the Homeostasis of the Intestinal Stem Cell Niche

2013 ◽  
Vol 144 (5) ◽  
pp. S-833
Author(s):  
Eva Martini ◽  
Nadine Wittkopf ◽  
Claudia Günther ◽  
Hitoshi Okada ◽  
Markus FF Neurath ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Niche ◽  
Intestinal Stem Cell ◽  
Cell Niche

scholarly journals Erratum: The HSP90 inhibitor, 17AAG, protects the intestinal stem cell niche and inhibits graft versus host disease development

Oncogene ◽  
2016 ◽  
Vol 35 (22) ◽  
pp. 2948-2948 ◽  
Author(s):  
A-L Joly ◽  
A Deepti ◽  
A Seignez ◽  
A Goloudina ◽  
S Hebrard ◽  
...  
Keyword(s):  
Stem Cell ◽  
Graft Versus Host Disease ◽  
Stem Cell Niche ◽  
Hsp90 Inhibitor ◽  
Intestinal Stem Cell ◽  
Disease Development ◽  
Host Disease ◽  
Graft Versus Host ◽  
Cell Niche

scholarly journals Rapid Crypt Cell Remodeling Regenerates the Intestinal Stem Cell Niche after Notch Inhibition

2020 ◽  
Vol 15 (1) ◽  
pp. 156-170 ◽  
Author(s):  
Natacha Bohin ◽  
Theresa M. Keeley ◽  
Alexis J. Carulli ◽  
Emily M. Walker ◽  
Elizabeth A. Carlson ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Niche ◽  
Crypt Cell ◽  
Intestinal Stem Cell ◽  
Cell Remodeling ◽  
Notch Inhibition ◽  
Cell Niche

scholarly journals Publisher Correction: Parasitic helminths induce fetal-like reversion in the intestinal stem cell niche

Nature ◽  
2018 ◽  
Vol 562 (7727) ◽  
pp. E22-E22 ◽  
Author(s):  
Ysbrand M. Nusse ◽  
Adam K. Savage ◽  
Pauline Marangoni ◽  
Axel K. M. Rosendahl-Huber ◽  
Tyler A. Landman ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Niche ◽  
Intestinal Stem Cell ◽  
Parasitic Helminths ◽  
Cell Niche
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