scholarly journals Targeted Gene Addition of Microdystrophin in Mice Skeletal Muscle via Human Myoblast Transplantation

2013 ◽  
Vol 2 ◽  
pp. e68 ◽  
Author(s):  
Basma F Benabdallah ◽  
Arnaud Duval ◽  
Joel Rousseau ◽  
Pierre Chapdelaine ◽  
Michael C Holmes ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Myoblast Transplantation ◽  
Human Myoblast

scholarly journals Dynamics of Myoblast Transplantation Reveal a Discrete Minority of Precursors with Stem Cell–like Properties as the Myogenic Source

1999 ◽  
Vol 144 (6) ◽  
pp. 1113-1122 ◽  
Author(s):  
Jonathan R. Beauchamp ◽  
Jennifer E. Morgan ◽  
Charles N. Pagel ◽  
Terence A. Partridge
Keyword(s):  
Cell Cycle ◽  
Skeletal Muscle ◽  
Tissue Culture ◽  
Stem Cell ◽  
Genetic Modification ◽  
Muscle Formation ◽  
Myoblast Transplantation ◽  
Dystrophic Mice

Myoblasts, the precursors of skeletal muscle fibers, can be induced to withdraw from the cell cycle and differentiate in vitro. Recent studies have also identified undifferentiated subpopulations that can self-renew and generate myogenic cells (Baroffio, A., M. Hamann, L. Bernheim, M.-L. Bochaton-Pillat, G. Gabbiani, and C.R. Bader. 1996. Differentiation. 60:47–57; Yoshida, N., S. Yoshida, K. Koishi, K. Masuda, and Y. Nabeshima. 1998. J. Cell Sci. 111:769–779). Cultured myoblasts can also differentiate and contribute to repair and new muscle formation in vivo, a capacity exploited in attempts to develop myoblast transplantation (MT) for genetic modification of adult muscle. Our studies of the dynamics of MT demonstrate that cultures of myoblasts contain distinct subpopulations defined by their behavior in vitro and divergent responses to grafting. By comparing a genomic and a semiconserved marker, we have followed the fate of myoblasts transplanted into muscles of dystrophic mice, finding that the majority of the grafted cells quickly die and only a minority are responsible for new muscle formation. This minority is behaviorally distinct, slowly dividing in tissue culture, but rapidly proliferative after grafting, suggesting a subpopulation with stem cell–like characteristics.

A New Immunodeficient Mouse Model for Human Myoblast Transplantation

2001 ◽  
Vol 12 (7) ◽  
pp. 823-831 ◽  
Author(s):  
R.N. Cooper ◽  
A. Irintchev ◽  
J.P. Di Santo ◽  
M. Zweyer ◽  
J.E. Morgan ◽  
...  
Keyword(s):  
Mouse Model ◽  
Immunodeficient Mouse ◽  
Myoblast Transplantation ◽  
Human Myoblast

scholarly journals Human Myoblast Fusion Requires Expression of Functional Inward Rectifier Kir2.1 Channels

2001 ◽  
Vol 153 (4) ◽  
pp. 677-686 ◽  
Author(s):  
Jacqueline Fischer-Lougheed ◽  
Jian-Hui Liu ◽  
Estelle Espinos ◽  
David Mordasini ◽  
Charles R. Bader ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Membrane Potential ◽  
Muscle Development ◽  
Myoblast Fusion ◽  
Inward Rectifier ◽  
Resting Membrane Potential ◽  
Skeletal Muscle Development ◽  
K Channels ◽  
Window Current ◽  
Human Myoblast

Myoblast fusion is essential to skeletal muscle development and repair. We have demonstrated previously that human myoblasts hyperpolarize, before fusion, through the sequential expression of two K+ channels: an ether-à-go-go and an inward rectifier. This hyperpolarization is a prerequisite for fusion, as it sets the resting membrane potential in a range at which Ca2+ can enter myoblasts and thereby trigger fusion via a window current through α1H T channels.

Utilization of an Antibody Specific for Human Dystrophin to Follow Myoblast Transplantation in Nude Mice

1993 ◽  
Vol 2 (2) ◽  
pp. 113-118 ◽  
Author(s):  
Johnny Huard ◽  
Geneviève Tremblay ◽  
Steve Verreault ◽  
Claude Labrecque ◽  
Jacques P. Tremblay
Keyword(s):  
Monoclonal Antibody ◽  
Nude Mice ◽  
Muscle Fibers ◽  
Dystrophin Gene ◽  
Mdx Mice ◽  
Myoblast Transplantation ◽  
Small Clusters ◽  
Human Dystrophin ◽  
Human Myoblast

Human myoblasts were transplanted in nude mice. The efficacy of these transplantations was analyzed using a monoclonal antibody (NCLDys3) specific for human dystrophin. This antibody did not reveal any dystrophin in nude mice that did not receive a human myoblast transplantation. However, about 30 days after a human myoblast transplantation, dystrophin-positive muscle fibers were observed. They were not abundant, and were present either in small clusters or isolated. This technique follows the fate of myoblast transplantation in animals that already have dystrophin, and distinguishes between new dystrophin-positive fibers due to the transplantation and the revertant fibers in mdx mice. Moreover, this technique does not require any labelling of the myoblasts before transplantation. It can also be used to detect dystrophin produced following the fusion of myoblasts transfected with the human dystrophin gene.

Human myoblast transplantation in immunodeficient and immunosuppressed mice: Evidence of rejection

1994 ◽  
Vol 17 (2) ◽  
pp. 224-234 ◽  
Author(s):  
Johnny Huard ◽  
Raynald Roy ◽  
Benoît Guérette ◽  
Steeve Verreault ◽  
Genevieve Trèmblay ◽  
...  
Keyword(s):  
Myoblast Transplantation ◽  
Human Myoblast ◽  
Immunosuppressed Mice
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