scholarly journals Human Myoblast Fusion Requires Expression of Functional Inward Rectifier Kir2.1 Channels

2001 ◽  
Vol 153 (4) ◽  
pp. 677-686 ◽  
Author(s):  
Jacqueline Fischer-Lougheed ◽  
Jian-Hui Liu ◽  
Estelle Espinos ◽  
David Mordasini ◽  
Charles R. Bader ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Membrane Potential ◽  
Muscle Development ◽  
Myoblast Fusion ◽  
Inward Rectifier ◽  
Resting Membrane Potential ◽  
Skeletal Muscle Development ◽  
K Channels ◽  
Window Current ◽  
Human Myoblast

Myoblast fusion is essential to skeletal muscle development and repair. We have demonstrated previously that human myoblasts hyperpolarize, before fusion, through the sequential expression of two K+ channels: an ether-à-go-go and an inward rectifier. This hyperpolarization is a prerequisite for fusion, as it sets the resting membrane potential in a range at which Ca2+ can enter myoblasts and thereby trigger fusion via a window current through α1H T channels.

scholarly journals Extracellular annexins and dynamin are important for sequential steps in myoblast fusion

2012 ◽  
Vol 200 (1) ◽  
pp. 109-123 ◽  
Author(s):  
Evgenia Leikina ◽  
Kamran Melikov ◽  
Sarmistha Sanyal ◽  
Santosh K. Verma ◽  
Bokkee Eun ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Development ◽  
Cell Metabolism ◽  
Myoblast Fusion ◽  
Skeletal Muscle Development ◽  
Crucial Step ◽  
Early Fusion ◽  
Additional Protein ◽  
Complete Cell ◽  
Multinucleated Myotubes

Myoblast fusion into multinucleated myotubes is a crucial step in skeletal muscle development and regeneration. Here, we accumulated murine myoblasts at the ready-to-fuse stage by blocking formation of early fusion intermediates with lysophosphatidylcholine. Lifting the block allowed us to explore a largely synchronized fusion. We found that initial merger of two cell membranes detected as lipid mixing involved extracellular annexins A1 and A5 acting in a functionally redundant manner. Subsequent stages of myoblast fusion depended on dynamin activity, phosphatidylinositol(4,5)bisphosphate content, and cell metabolism. Uncoupling fusion from preceding stages of myogenesis will help in the analysis of the interplay between protein machines that initiate and complete cell unification and in the identification of additional protein players controlling different fusion stages.

scholarly journals Tks5 and Dynamin-2 enhance actin bundle rigidity in invadosomes to promote myoblast fusion

2019 ◽  
Vol 218 (5) ◽  
pp. 1670-1685 ◽  
Author(s):  
Mei-Chun Chuang ◽  
Shan-Shan Lin ◽  
Ryosuke L. Ohniwa ◽  
Gang-Hui Lee ◽  
You-An Su ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Actin Filaments ◽  
Driving Force ◽  
Muscle Development ◽  
Myoblast Fusion ◽  
Structural Function ◽  
Skeletal Muscle Development ◽  
Actin Bundle ◽  
Isoform Switching ◽  
Dynamin 2

Skeletal muscle development requires the cell–cell fusion of differentiated myoblasts to form muscle fibers. The actin cytoskeleton is known to be the main driving force for myoblast fusion; however, how actin is organized to direct intercellular fusion remains unclear. Here we show that an actin- and dynamin-2–enriched protrusive structure, the invadosome, is required for the fusion process of myogenesis. Upon differentiation, myoblasts acquire the ability to form invadosomes through isoform switching of a critical invadosome scaffold protein, Tks5. Tks5 directly interacts with and recruits dynamin-2 to the invadosome and regulates its assembly around actin filaments to strengthen the stiffness of dynamin-actin bundles and invadosomes. These findings provide a mechanistic framework for the acquisition of myogenic fusion machinery during myogenesis and reveal a novel structural function for Tks5 and dynamin-2 in organizing actin filaments in the invadosome to drive membrane fusion.

scholarly journals Human Myoblast Differentiation: Ca2+ Channels are Activated by K+ Channels

Physiology ◽  
2002 ◽  
Vol 17 (1) ◽  
pp. 22-26 ◽  
Author(s):  
Laurent Bernheim ◽  
Charles R. Bader
Keyword(s):  
Membrane Potential ◽  
Cellular Differentiation ◽  
Myoblast Fusion ◽  
Myoblast Differentiation ◽  
K Channels ◽  
Intracellular Ca ◽  
Human Myoblast ◽  
Ca2 Channels

In a paradigm of cellular differentiation, human myoblast fusion, we investigated how a Ca2+ influx, indispensable for fusion, is triggered. We show how newly expressed Kir2.1 K+ channels, via their hyperpolarizing effect on the membrane potential, generate a window Ca2+ current (mediated by a1H T-type Ca2+ channels), which causes intracellular Ca2+ to rise.

scholarly journals Key Genes Regulating Skeletal Muscle Development and Growth in Farm Animals

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 835
Author(s):  
Mohammadreza Mohammadabadi ◽  
Farhad Bordbar ◽  
Just Jensen ◽  
Min Du ◽  
Wei Guo
Keyword(s):  
Skeletal Muscle ◽  
Candidate Genes ◽  
Meat Quality ◽  
Muscle Development ◽  
Muscle Growth ◽  
Farm Animals ◽  
Meat Production ◽  
Skeletal Muscle Development ◽  
Extrinsic Factors ◽  
Myogenic Regulatory Factor

Farm-animal species play crucial roles in satisfying demands for meat on a global scale, and they are genetically being developed to enhance the efficiency of meat production. In particular, one of the important breeders’ aims is to increase skeletal muscle growth in farm animals. The enhancement of muscle development and growth is crucial to meet consumers’ demands regarding meat quality. Fetal skeletal muscle development involves myogenesis (with myoblast proliferation, differentiation, and fusion), fibrogenesis, and adipogenesis. Typically, myogenesis is regulated by a convoluted network of intrinsic and extrinsic factors monitored by myogenic regulatory factor genes in two or three phases, as well as genes that code for kinases. Marker-assisted selection relies on candidate genes related positively or negatively to muscle development and can be a strong supplement to classical selection strategies in farm animals. This comprehensive review covers important (candidate) genes that regulate muscle development and growth in farm animals (cattle, sheep, chicken, and pig). The identification of these genes is an important step toward the goal of increasing meat yields and improves meat quality.

Plectin regulates Wnt signaling mediated-skeletal muscle development by interacting with Dishevelled-2 and antagonizing autophagy

Gene ◽  
2021 ◽  
Vol 783 ◽  
pp. 145562
Author(s):  
Huadong Yin ◽  
Shunshun Han ◽  
Can Cui ◽  
Yan Wang ◽  
Diyan Li ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Wnt Signaling ◽  
Muscle Development ◽  
Skeletal Muscle Development

scholarly journals Depletion of the p43 mitochondrial T3 receptor in mice affects skeletal muscle development and activity

2011 ◽  
Vol 26 (2) ◽  
pp. 748-756 ◽  
Author(s):  
Laurence Pessemesse ◽  
Audrey Schlernitzauer ◽  
Chamroeun Sar ◽  
Jonathan Levin ◽  
Stéphanie Grandemange ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Development ◽  
Skeletal Muscle Development

scholarly journals MiR-206, a Key Modulator of Skeletal Muscle Development and Disease

2015 ◽  
Vol 11 (3) ◽  
pp. 345-352 ◽  
Author(s):  
Guoda Ma ◽  
Yajun Wang ◽  
You Li ◽  
Lili Cui ◽  
Yujuan Zhao ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Development ◽  
Skeletal Muscle Development
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