scholarly journals Relationship Between the Physicochemical Properties of Lipid Nanoparticles and the Quality of siRNA Delivery to Liver Cells

2016 ◽  
Vol 24 (4) ◽  
pp. 788-795 ◽  
Author(s):  
Yusuke Sato ◽  
Hiroto Hatakeyama ◽  
Mamoru Hyodo ◽  
Hideyoshi Harashima
Keyword(s):  
Physicochemical Properties ◽  
Sirna Delivery ◽  
Lipid Nanoparticles ◽  
Liver Cells

scholarly journals Shielding of Lipid Nanoparticles for siRNA Delivery: Impact on Physicochemical Properties, Cytokine Induction, and Efficacy

2014 ◽  
Vol 3 ◽  
pp. e210 ◽  
Author(s):  
Varun Kumar ◽  
June Qin ◽  
Yongfeng Jiang ◽  
Richard G Duncan ◽  
Benjamin Brigham ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Sirna Delivery ◽  
Lipid Nanoparticles ◽  
Cytokine Induction

scholarly journals Elucidation of the physicochemical properties and potency of siRNA-loaded small-sized lipid nanoparticles for siRNA delivery

2016 ◽  
Vol 229 ◽  
pp. 48-57 ◽  
Author(s):  
Yusuke Sato ◽  
Yusuke Note ◽  
Masatoshi Maeki ◽  
Noritada Kaji ◽  
Yoshinobu Baba ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Sirna Delivery ◽  
Lipid Nanoparticles

scholarly journals Physicochemical Characterization Cascade of Nanoadjuvant–Antigen Systems for Improving Vaccines

Vaccines ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 544
Author(s):  
Giuditta Guerrini ◽  
Antonio Vivi ◽  
Sabrina Gioria ◽  
Jessica Ponti ◽  
Davide Magrì ◽  
...  
Keyword(s):  
Immune Response ◽  
Protein Structure ◽  
Physicochemical Properties ◽  
Physicochemical Characterization ◽  
Protein Antigen ◽  
Efficacy And Safety

Adjuvants have been used for decades to enhance the immune response to vaccines, in particular for the subunit-based adjuvants. Physicochemical properties of the adjuvant-protein antigen complexes, such as size, morphology, protein structure and binding, influence the overall efficacy and safety of the vaccine. Here we show how to perform an accurate physicochemical characterization of the nanoaluminum–ovalbumin complex. Using a combination of existing techniques, we developed a multi-staged characterization strategy based on measurements of increased complexity. This characterization cascade has the advantage of being very flexible and easily adaptable to any adjuvant-protein antigen combinations. It will contribute to control the quality of antigen–adjuvant complexes and immunological outcomes, ultimately leading to improved vaccines.

scholarly journals Comparison of Morphological and Physicochemical Properties of a Floury Rice Variety upon Pre-Harvest Sprouting

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 746
Author(s):  
Chae-Min Han ◽  
Jong-Hee Shin ◽  
Jung-Bae Kwon ◽  
Jong-Soo Kim ◽  
Jong-Gun Won ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Rice Variety ◽  
Wheat Variety ◽  
Economic Loss ◽  
Rice Grain ◽  
Starch Granules ◽  
Rice Varieties ◽  
Starch Structure ◽  
Gelatinization Properties

Pre-harvest sprouting (PHS) severely reduces rice grain yield, significantly affects grain quality, and leads to substantial economic loss. In this study, we aimed to characterize the physicochemical properties and processing quality of the Garumi 2 flour rice variety under PHS conditions and compare them with those of the Seolgaeng, Hangaru, Shingil, and Ilpum rice varieties and the Keumkang wheat variety. Analysis of the molecular structure of starch revealed uniform starch granules, increased proportions of short-chain amylopectin in DP 6–12 (51.0–55.3%), and enhanced crystallinity (30.7–35.7%) in rice varieties for flour compared with the Ilpum cooking rice variety. PHS significantly altered the starch structure and gelatinization properties of Garumi 2. It also caused surface pitting and roughness in Garumi 2 starch granules and decreased their crystallinity. Collectively, the findings of this study provide important novel insights into the effects of PHS on the physicochemical properties of Garumi 2 floury rice for flour.

scholarly journals Lipid-Based Nanoparticles in the Clinic and Clinical Trials: From Cancer Nanomedicine to COVID-19 Vaccines

Vaccines ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 359
Author(s):  
Thai Thanh Hoang Thi ◽  
Estelle J. A. Suys ◽  
Jung Seok Lee ◽  
Dai Hai Nguyen ◽  
Ki Dong Park ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Clinical Trials ◽  
Physicochemical Properties ◽  
Solid Lipid Nanoparticles ◽  
Drug Delivery Systems ◽  
Lipid Nanoparticles ◽  
Therapeutic Vaccines ◽  
Cancer Nanomedicine ◽  
Fundamental Research ◽  
Further Development

COVID-19 vaccines have been developed with unprecedented speed which would not have been possible without decades of fundamental research on delivery nanotechnology. Lipid-based nanoparticles have played a pivotal role in the successes of COVID-19 vaccines and many other nanomedicines, such as Doxil® and Onpattro®, and have therefore been considered as the frontrunner in nanoscale drug delivery systems. In this review, we aim to highlight the progress in the development of these lipid nanoparticles for various applications, ranging from cancer nanomedicines to COVID-19 vaccines. The lipid-based nanoparticles discussed in this review are liposomes, niosomes, transfersomes, solid lipid nanoparticles, and nanostructured lipid carriers. We particularly focus on the innovations that have obtained regulatory approval or that are in clinical trials. We also discuss the physicochemical properties required for specific applications, highlight the differences in requirements for the delivery of different cargos, and introduce current challenges that need further development. This review serves as a useful guideline for designing new lipid nanoparticles for both preventative and therapeutic vaccines including immunotherapies.

scholarly journals SIFAT FISIKOKIMIA HAND AND BODY CREAM DENGAN PEMANFAATAN EKSTRAK ETANOL BUNGA GEMITIR (Tagetes erecta L.) DAN BUNGA PACAR AIR MERAH (Impatiens balsamina L.) DARI LIMBAH CANANG

2017 ◽  
Vol 5 (1) ◽  
pp. 1
Author(s):  
Dewa Ayu Ika Paramitha ◽  
James Sibarani ◽  
Ni Made Suaniti
Keyword(s):  
Antioxidant Activity ◽  
Physicochemical Properties ◽  
National Standard ◽  
Tagetes Erecta ◽  
Basic Formula ◽  
Impatiens Balsamina ◽  
Ethanol Extracts ◽  
Tagetes Erecta L ◽  
Microbial Numbers

ABSTRAK: Pada penelitian ini dilakukan pengembangan formula sediaan hand and body cream dengan memanfaatkan sumber antioksidan yang dimiliki oleh dua jenis ekstrak etanol yaitu ekstrak bunga pacar air merah (Impatiens balsamina L.) dan bunga gemitir (Tagetes erecta L.) dengan taraf perlakuan sebesar 1% dari masing-masing ekstrak (FPM dan FG) dan campuran 0,5% ekstrak pacar air merah dan 0,5% ekstrak gemitir (FCPMG). Pengujian terhadap  produk dilakukan pada sifat fisikokimia  meliputi pH, viskositas, aktivitas antioksidan, angka lempeng total mikroba, dan ukuran partikel. Berdasarkan hasil pengujian produk hand and body cream yang dihasilkan memiliki antioksidan yang cukup tinggi sebagai produk perawatan tubuh dengan penambahan 1% ekstrak etanol pacar air merah, gemitir dan campuran dari kedua ekstrak etanol. Kualitas produk hand and body cream yang dihasilkan baik dan memenuhi standar mutu SNI dari aspek nilai pH, viskositas, dan nilai ALT. Warna yang dihasilkan mulai dari warna kuning kemerahan sampai dengan warna kuning, dan partikel yang dimiliki oleh produk adalah nanopartikel dengan sistem polidispersi. ABSTRACT: Formulations of hand and body creams using ethanol extracts of flowers of red Impatiens balsamina L. And Tagetes erecta L. As antioxidant sources have been developed.  One percent of  extracts (FPM and FG) and the mixture of 0.5% each extracts (FCPMG) were added to the basic formula. The analysis of physicochemical properties of the products was conducted including pH, viscosity, antioxidant activity, the size of the particles, and total microbial plate numbers. Based on the results, the quality of the hand and body creams meet the national standard of Indonesia (SNI) regarding to pH, viscosity, and the total microbial numbers. The colours of the hand and body creams resulted are reddish yellow to yellow with polydispersion nanoparticles.

scholarly journals Development of siRNA-Loaded Lipid Nanoparticles Targeting Long Non-Coding RNA LINC01257 as a Novel and Safe Therapeutic Approach for t(8;21) Pediatric Acute Myeloid Leukemia

Pharmaceutics ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1681
Author(s):  
Patrick Connerty ◽  
Ernest Moles ◽  
Charles E. de Bock ◽  
Nisitha Jayatilleke ◽  
Jenny L. Smith ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Acute Myeloid Leukemia ◽  
Myeloid Leukemia ◽  
Mononuclear Cells ◽  
Sirna Delivery ◽  
Standard Of Care ◽  
Lipid Nanoparticles ◽  
Protein Product ◽  
Peripheral Blood Mononuclear ◽  
Acute Myeloid

Standard of care therapies for children with acute myeloid leukemia (AML) cause potent off-target toxicity to healthy cells, highlighting the need to develop new therapeutic approaches that are safe and specific for leukemia cells. Long non-coding RNAs (lncRNAs) are an emerging and highly attractive therapeutic target in the treatment of cancer due to their oncogenic functions and selective expression in cancer cells. However, lncRNAs have historically been considered ‘undruggable’ targets because they do not encode for a protein product. Here, we describe the development of a new siRNA-loaded lipid nanoparticle for the therapeutic silencing of the novel oncogenic lncRNA LINC01257. Transcriptomic analysis of children with AML identified LINC01257 as specifically expressed in t(8;21) AML and absent in healthy patients. Using NxGen microfluidic technology, we efficiently and reproducibly packaged anti-LINC01257 siRNA (LNP-si-LINC01257) into lipid nanoparticles based on the FDA-approved Patisiran (Onpattro®) formulation. LNP-si-LINC01257 size and ζ-potential were determined by dynamic light scattering using a Malvern Zetasizer Ultra. LNP-si-LINC01257 internalization and siRNA delivery were verified by fluorescence microscopy and flow cytometry analysis. lncRNA knockdown was determined by RT-qPCR and cell viability was characterized by flow cytometry-based apoptosis assay. LNP-siRNA production yielded a mean LNP size of ~65 nm with PDI ≤0.22 along with a >85% siRNA encapsulation rate. LNP-siRNAs were efficiently taken up by Kasumi-1 cells (>95% of cells) and LNP-si-LINC01257 treatment was able to successfully ablate LINC01257 expression which was accompanied by a significant 55% reduction in total cell count following 48 h of treatment. In contrast, healthy peripheral blood mononuclear cells (PBMCs), which do not express LINC01257, were unaffected by LNP-si-LINC01257 treatment despite comparable levels of LNP-siRNA uptake. This is the first report demonstrating the use of LNP-assisted RNA interference modalities for the silencing of cancer-driving lncRNAs as a therapeutically viable and non-toxic approach in the management of AML.

scholarly journals Soil quality of the tea and apple-growing sites in Mindat District, Chin state, Myanmar: A preliminary study

2021 ◽  
Vol 70 (1) ◽  
pp. 27-40
Author(s):  
Yaung Kwee ◽  
Khin Soe
Keyword(s):  
Physicochemical Properties ◽  
Silty Clay ◽  
Subtropical Climate ◽  
Clay Loam ◽  
Silty Clay Loam ◽  
Preliminary Study ◽  
Phosphorus And Potassium ◽  
Very High ◽  
Physicochemical Properties Of Soils

In this study, two sites from tea and apple growing sites were collected from Pyay village and Nine Mile village, Mindat district, Chin state of West Myanmar under a humid subtropical climate. The results of physicochemical properties of observed soils were neutral pH, favorable moisture, silty clay loam texture, very high content of organic carbon, organic matter and total nitrogen. However, the tea growing soil was very poor in phosphorus and potassium content. Moreover, both soils lack of available potassium. The content of heavy metals in both soils was not varied from each other and followed the order: Fe (iron) > Cu (copper) >Zn (zinc) > Pb (lead) > Cr (chromium) and was below the maximum allowed concentrations (MAC). Therefore, the studied soils are generally favorable for cultivation under the condition of application of phosphorus and potassium fertilizers. However, due to the regular application of fertilizers and pesticides, it is necessary to monitor these soils for PTE levels. Further research is recommended, which must include analyses of the physicochemical properties of soils to a two depths of 0-30 and 30-60 cm, especially for the area where fruit plants are grown. In addition, higher density of soil samples and sub-samples are necessary to produce a reliable dataset that will allow proper statistical analysis.

scholarly journals Effect of Foliage Application with Boron-Containing Chelate Complexones on Productivity and Quality of Flax Products

2021 ◽  
pp. 11-15
Author(s):  
А.И. Беленков ◽  
В.Н. Мельников ◽  
А.А. Петрова ◽  
Т.И. Смирнова ◽  
М.Н. Павлов
Keyword(s):  
Trace Elements ◽  
Physicochemical Properties ◽  
Field Test ◽  
Growing Season ◽  
Single Factor ◽  
Environmentally Safe ◽  
Plant Yields ◽  
Quality Of Products ◽  
Phenological Phases

В условиях двухлетнего полевого опыта на растениях льна-долгунца исследовано влияние внекорневой подкормки боросодежащими хелатными комплексонами на продуктивность и качество льнопродукции. Изучено действие борат-иминодисукцината (В-ИДЯК) и борат-этилендиаминдисукцината (В-ЭДДЯК) в сравнении с борной кислотой и борат-этилендиаминтетраацетатом (В-ЭДТУК). Исследования выполнили в 2019–2020 гг. в однофакторном полевом опыте. Установлено, что наиболее эффективное и экологически безопасное влияние оказывает внекорневая подкормка посевов льна-долгунца хелатным комплексом В-ЭДДЯК. При внекорневой подкормке часть препарата, попадая в почву, мобилизует также другие микроэлементы, тем самым оказывая стимулирующее действие на растения. Полученные результаты показали, что внекорневая подкормка боросодержащими соединениями существенно повлияла на накопление биомассы льна-долгунца. Выявлена положительная динамика влияния боросодержащих комплексов на прохождение фенологических фаз во второй половине вегетации. Внекорневая подкормка растений ускорила цветение льна, которое наступало раньше на 2–3 дня по сравнению с контролем. Применение подкормки хелатными боросодержащими комплексонами способствует росту урожайности растений, повышению качества продукции, в части улучшения физико-химических свойств льноволокна. Прибавка урожая к контролю составила: по льносоломе – 8,3–15,7% (3,2–6 ц/га), семенам – 38,4–56% (1,2–1,9 ц/га). Техническая длина соломы была выше у всех вариантов опыта на 8,5–10,4% по сравнению с контролем. При этом увеличивались: выход луба, прочность, пригодность и номер льносоломы. Увеличение содержания луба составило от 1,6 до 4,5%. Under the conditions of two-year field test, the effects of foliage application with boron-containing chelate complexones on the productivity and quality of flax products were investigated on long-stalked flax plants. The effect of borate-iminodisuccinate (B-IDSA) and borate-ethylene diamin-disuccinate (B-EDDSA) in comparison with boric acid and borate-ethylene diamintetraacetate (B-EDTAA) was studied. The researches were conducted in 2019–2020 in a single-factor field test. It has been found that the most effective and environmentally safe influence is exerted by foliage application of long-stalked flax sowings with the chelate complex B-EDDSA. In foliage application part of the preparation falling into the soil also mobilizes other trace elements, thereby exerting a stimulating effect on plants. The obtained results showed that foliage application with boron-containing compounds had a significant impact on the accumulation of long-stalked flax biomass. Positive dynamics of boron-containing complexes influence on phenological phases passage in the second half of growing season has been revealed. Foliage application of plants accelerated flax flowering, which occurred earlier by 2–3 days compared to control. The use of top-dressing with chelated boron-containing complexones contributes to the growth of plant yields, an increase in the quality of products, in terms of improving the physicochemical properties of flax fiber. The increase in the yield to control was: according to linen straw – 8.3–15.7% (3.2–6 c/ha), seeds – 38.4–56% (1.2–1.9 c/ha). The technical length of the straw was 8.5–10.4% higher in all experience variants compared to the control. At the same time the output of the bast, the strength, suitability and number of the linen straw have been increased. The increase in bast content ranged from 1.6 to 4.5%.

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