scholarly journals Intracellular Tracking of Single-plasmid DNA Particles After Delivery by Electroporation

2013 ◽  
Vol 21 (12) ◽  
pp. 2217-2226 ◽  
Author(s):  
Christelle Rosazza ◽  
Annette Buntz ◽  
Thorsten Rieß ◽  
Dominik Wöll ◽  
Andreas Zumbusch ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Single Plasmid

scholarly journals Low-cost fabrication of centimetre-scale periodic arrays of single plasmid DNA molecules

Lab on a Chip ◽  
2013 ◽  
Vol 13 (17) ◽  
pp. 3367 ◽  
Author(s):  
Brett Kirkland ◽  
Zhibin Wang ◽  
Peipei Zhang ◽  
Shin-ichiro Takebayashi ◽  
Steven Lenhert ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Low Cost ◽  
Dna Molecules ◽  
Periodic Arrays ◽  
Single Plasmid

Characterization and cloning of a 37.6-kb plasmid carried byLegionella pneumophilarecovered from patients and hospital water over a 12-year period

1997 ◽  
Vol 43 (2) ◽  
pp. 193-197 ◽  
Author(s):  
Shawn D. Mansfield ◽  
Greg. S. Bezanson ◽  
Thomas J. Marrie
Keyword(s):  
Legionella Pneumophila ◽  
Plasmid Dna ◽  
Potable Water ◽  
Pattern Analysis ◽  
Plasmid Stability ◽  
Physical Map ◽  
Fragmentation Pattern ◽  
Single Plasmid ◽  
Double Digestion ◽  
Victoria General Hospital

For 12 years, strains of Legionella pneumophila serogroup 1 harbouring a 37.6-kb (23 MDa) plasmid have predominated among patient and potable water isolates at the Victoria General Hospital, Halifax, N.S. Plasmid DNA recovered from 24 strains isolated between 1983 and 1995 was digested with the restriction endonucleases EcoRI, HindIII, KpnI, PvuII, XbaI, and BamHI. The distribution of cutting sites indicated that the 23-MDa size group had remained essentially unchanged during this period, suggesting the persistence of a single plasmid type. Further fragmentation pattern analysis permitted the construction of a physical map of the prototype 23-MDA plasmid, pLp4269. Double digestion with BamHI–HindIII enabled the cloning of 94.4% of pLp4269 into pBluescript vector. A 2.1-kb fragment was not clonable. Plasmid pLp4269 is the first of the smaller Legionella extrachromosomal DNAs to be characterized in this way.Key words: Legionella, plasmid, stability, map, cloning.

Evaluation of biocompatibility and efficiency of plasmid DNA delivery by gene-activated hydrogels in vitro

2019 ◽  
Vol XIV (2) ◽  
Author(s):  
I.Y. Bozo ◽  
A.A. Titova ◽  
M.N. Zhuravleva ◽  
A.I. Bilyalov ◽  
M.O. Mavlikeev ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Dna Delivery

Detection of Rhizoctonia solani AG-2-2 IV, the Causal Agent of Large Patch of Zoysiagrass, Using Plasmid DNA as a Probe.

1998 ◽  
Vol 64 (5) ◽  
pp. 451-457 ◽  
Author(s):  
Susumu TAKAMATSU ◽  
Manami NAKANO ◽  
Hideyuki YOKOTA ◽  
Hitoshi KUNOH
Keyword(s):  
Rhizoctonia Solani ◽  
Plasmid Dna ◽  
Causal Agent ◽  
Large Patch
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