scholarly journals Hepatic Insulin Gene Therapy Normalizes Diurnal Fluctuation of Oxidative Metabolism in Diabetic BB/Wor Rats

2008 ◽  
Vol 16 (7) ◽  
pp. 1235-1242 ◽  
Author(s):  
Darin E Olson ◽  
Adam G Campbell ◽  
Marty H Porter ◽  
Kimberly G Freeman ◽  
Emily Kelso ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Oxidative Metabolism ◽  
Insulin Gene ◽  
Diurnal Fluctuation

PERMANENT REVERSAL OF AUTOIMMUNE DIABETES IN NON OBESE DIABETIC MICE AFTER LENTIVIRAL HEPATIC INSULIN GENE THERAPY

2008 ◽  
Vol 86 (Supplement) ◽  
pp. 11
Author(s):  
B Ren ◽  
B OʼBrien ◽  
M Byrne ◽  
E Chʼng ◽  
M Swan ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Autoimmune Diabetes ◽  
Insulin Gene ◽  
Diabetic Mice

Current strategies and perspectives in insulin gene therapy for diabetes

2007 ◽  
Vol 2 (1) ◽  
pp. 27-34 ◽  
Author(s):  
Dimitry A Chistiakov ◽  
Inna Tyurina
Keyword(s):  
Gene Therapy ◽  
Insulin Gene

scholarly journals Long-term correction of diabetes in rats after lentiviral hepatic insulin gene therapy

Diabetologia ◽  
2007 ◽  
Vol 50 (9) ◽  
pp. 1910-1920 ◽  
Author(s):  
B. Ren ◽  
B. A. O’Brien ◽  
M. A. Swan ◽  
M. E. Koina ◽  
N. Nassif ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Insulin Gene

HEPATOCYTE BASED INSULIN GENE THERAPY

2008 ◽  
Vol 86 (Supplement) ◽  
pp. 109-110
Author(s):  
H Sollinger ◽  
T Alam ◽  
D Held ◽  
E Forsberg
Keyword(s):  
Gene Therapy ◽  
Insulin Gene

scholarly journals Genetically Engineered Insulin Mediated by Glucose Transporter-2 (GLUT2) Promoter for the Biosynthesis of Insulin in Rat Hepatocytes: Insulin Gene Therapy

2021 ◽  
Author(s):  
Gehad Abdallah
Keyword(s):  
Gene Therapy ◽  
Pancreatic Beta Cells ◽  
Glucose Transporter ◽  
Glucose Sensing ◽  
Genetically Engineered ◽  
Insulin Gene ◽  
Site Directed Mutagenesis ◽  
Target Cells ◽  
Insulin Production ◽  
Glucose Transporter 2

Abstract Background: Expression of insulin in hepatocytes by hepatic gene therapy is a promising treatment of diabetes. The conversion of immature proinsulin to mature insulin occurs only in cells that contain the enzymes responsible for the cleavage of proinsulin to insulin.Results: I engineered rat proinsulin with the sites of cleavage (Furin Cleavable Sites) using site directed mutagenesis for removal of C-peptide to form the two chains A and B for mature insulin production. This engineered proinsulin was constructed into a non-viral expressing vector and regulated by glucose transporter-2 promoter to control the amount of mature insulin expressed, and to modulate the amount of glucose found in hepatocytes. The mature, active and regulated expressed insulin was secreted according to the amount of glucose regulated by the glucose transporter-2 promoter. Concolusion: For successful hepatic insulin gene therapy, insulin production must be tightly coupled to glucose concentration. Hepatocytes are excellent target cells for insulin gene therapy since, they are similar to pancreatic beta cells, they have the ability to rapidly adapt to blood glucose concentrations as they possess glucose-sensing components, such as Glucose Transporter-2.

scholarly journals Genome engineering and disease modeling via programmable nucleases for insulin gene therapy; promises of CRISPR/Cas9 technology

2021 ◽  
Vol 13 (6) ◽  
pp. 485-502
Author(s):  
Yunus E Eksi ◽  
Ahter D Sanlioglu ◽  
Bahar Akkaya ◽  
Bilge Esin Ozturk ◽  
Salih Sanlioglu
Keyword(s):  
Gene Therapy ◽  
Genome Engineering ◽  
Disease Modeling ◽  
Insulin Gene ◽  
Programmable Nucleases

scholarly journals Lentivirus Mediated Pancreatic Beta-Cell-Specific Insulin Gene Therapy for STZ-Induced Diabetes

2020 ◽  
Author(s):  
Fulya Erendor ◽  
Yunus Emre Eksi ◽  
Elif Ozgecan Sahin ◽  
Mustafa Kemal Balci ◽  
Thomas S. Griffith ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Beta Cell ◽  
Pancreatic Beta Cell ◽  
Insulin Gene ◽  
Specific Insulin

Glucose- and Metabolically Regulated Hepatic Insulin Gene Therapy for Diabetes

2008 ◽  
Vol 25 (6) ◽  
pp. 1460-1468 ◽  
Author(s):  
Paul Yueh-Jen Hsu ◽  
Robert M. Kotin ◽  
Ya-Wun Yang
Keyword(s):  
Gene Therapy ◽  
Insulin Gene
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