Removal of subepithelial immune complexes with excess unaltered or cationic antigen

Lawrence Y.C. Agodoa; Mart Mannik

doi:10.1038/ki.1987.165

In situ Formation of Subepithelial Immune Complexes in the Rabbit Glomerulus: Requirement of a Cationic Antigen

Nephron ◽

10.1159/000183165 ◽

1984 ◽

Vol 36 (4) ◽

pp. 257-264 ◽

Cited By ~ 11

Author(s):

Harry J. Ward ◽

Arthur H. Cohen ◽

Wayne A. Border

Keyword(s):

Immune Complexes ◽

In Situ Formation ◽

Cationic Antigen

Download Full-text

A Sensitive On-Grid Immunoelectron Microscopic Procedure for Diagnosis of Rotavirus Infection

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s143192760000221x ◽

1980 ◽

Vol 38 ◽

pp. 506-507

Author(s):

M. F. Miller ◽

A. R. Rubenstein

Keyword(s):

Electron Microscopy ◽

Specific Antibody ◽

Immune Complexes ◽

Acute Gastroenteritis ◽

Plant Viruses ◽

Aggregation Process ◽

Microscopic Technique ◽

Fecal Material ◽

Present Communication ◽

Number Of Particles

Studies of rotavirus particles in humans, monkeys and various non-primates with acute gastroenteritis have involved detection of virus in fecal material by electron microscopy. The EM techniques most commonly employed have been the conventional negative staining (Fig. 1) and immune aggregation (Fig. 2) procedures. Both methods are somewhat insensitive and can most reliably be applied to samples containing large quantities of virus either naturaLly or as a result of concentration by ultracentrifugation. The formation of immune complexes by specific antibody in the immune aggregation procedures confirms the rotavirus diagnosis, but the number of particles per given microscope field is effectively reduced by the aggregation process. In the present communication, we describe use of an on-grid immunoelectron microscopic technique in which rotavirus particles are mounted onto microscope grids that were pre-coated with specific antibody. The technique is a modification of a method originalLy introduced by Derrick (1) for studies of plant viruses.

Download Full-text

Localization of Immune Complexes in the Basement Membrane of the Ductuli Efferentes of the Rhesus Monkey

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s1431927600001963 ◽

1980 ◽

Vol 38 ◽

pp. 456-457

Author(s):

D. Marsh

Keyword(s):

Basement Membrane ◽

Fluorescence Microscopy ◽

Rhesus Monkey ◽

Immune Complex ◽

Immune Complexes ◽

Vas Deferens ◽

Frozen Sections ◽

Efferent Ducts ◽

Complex Deposition ◽

Sperm Antibodies

As a result of vasectomy, spermatozoa are confined to the epididymis and vas deferens, where they degenerate, releasing antigens that enter the circulation or are engulfed by macrophages. Multiple antigens of the sperm can elicit production of autoantibodies; circulating anti-sperm antibodies are found in a large percentage of vasectomized men, indicating the immunogenicity of the sperm. The increased prevalence of macrophages in the liomen of the rhesus monkey testicular efferent ducts after vasectomy led to further study of this region. Frozen sections were used for evaluation of immunopathological status by fluorescence microscopy with fluorescein-conjugated antibody. Subsequent granular deposits of immune complexes were revealed by positive immunofluorescence staining for complement. The immune complex deposition in the basement membrane surrounding the efferent ducts implies that this region is involved in antigen leakage (Fig. 1).

Download Full-text

Coagulation and Fibrinolysis Study in Systemic Lupus erythematosus: Haematological, Urinary and Tissue Parameters

Thrombosis and Haemostasis ◽

10.1055/s-0038-1653421 ◽

1981 ◽

Vol 46 (03) ◽

pp. 575-580 ◽

Cited By ~ 3

Author(s):

P Stratta ◽

C Canavese ◽

P Valmaggia ◽

M Rotunno ◽

E Levi ◽

...

Keyword(s):

Systemic Lupus Erythematosus ◽

Lupus Erythematosus ◽

Immune Complexes ◽

Acute Stage ◽

Systemic Lupus ◽

Immunological Activity ◽

Coagulation Abnormalities ◽

Coagulation And Fibrinolysis

SummaryHaematochemical, urinary and tissue parameters were examined in the elaboration of the coagulation and fibrinolysis profile in 33 cases of systemic lupus erythematosus in different stages of the disease. Coagulation abnormalities varied from hypo- to hyper-coagulabitity, these being often associated in the same patient, either simultaneously or at different stages of the disease. Activation of coagulation, closely related to the immunological activity of the disease, was present in 80% cases in the acute stage, and 36% of those in the remission stage. The lupus-like anticoagulant was not much involved, and platelets were the prime figures in the haemostatic abnormalities of lupus, those being the preferred target of direct antibody activities, or possibly of immune complexes as well. Activation of the coagulatory cascade is not uncommonly accompanied by a thrombophilic tendency coupled with signs of consumption, this being the expression of a continuously stimulated haemostatic balance.

Download Full-text

Effect of Cyclic AMP and Cyclic GMP on Thromboplastin (Factor III) Synthesis in Human Monocytes In Vitro

Thrombosis and Haemostasis ◽

10.1055/s-0038-1665317 ◽

1983 ◽

Vol 50 (04) ◽

pp. 804-809 ◽

Cited By ~ 16

Author(s):

Torstein Lyberg

Keyword(s):

Cyclic Amp ◽

Cyclic Gmp ◽

Immune Complexes ◽

Phosphodiesterase Inhibitors ◽

Inhibitory Effect ◽

Intracellular Camp ◽

Human Monocytes ◽

Purified Protein Derivative ◽

A 23187

SummaryHuman monocytes in vitro respond to various agents (immune complexes, lectins, endotoxin, the divalent ionophore A 23187, 12-0-tetradecanoyl-phorbol 13-acetate [TPA], purified protein derivative [PPD] of Bacille Calmette-Guerin) with an increased synthesis of the protein component of thromboplastin. The effect of cyclic AMP and cyclic GMP on this response has been studied. Dibutyryl-cyclic AMP, prostaglandin E1 and the phosphodiesterase inhibitors 3-butyl-1-methyl-xanthine (MIX) and rac -4-(3-butoxy-4-methoxybenzyl)-2-imidazolidinone (Ro 201724), separately and in combination have a pronounced inhibitory effect on the response to immune complexes and PPD, and a moderate effect on the response to endotoxin and lectins. The effect on TPA response and on the response to A 23187 was slight. Dibutyryl-cyclic GMP (1 mM) gave a slight inhibition of the TPA arid IC response, but had essentially no effect on the response to other inducers. The intracellular cAMP level increased when monocytes were incubated with IC, TPA or A 23187 followed by a decrease to basal levels within 1-2 hr, whereas lectin (PHA) and PPD did not induce such changes. The cAMP response to endotoxin varied. Stimulation with IC induced an increase in monocyte cGMP levels, whereas the other stimulants did not cause such changes.

Download Full-text

Studies on the Binding of Proteins to the Human Platelet Surface: Relation to Platelet Activation

Thrombosis and Haemostasis ◽

10.1055/s-0038-1661315 ◽

1985 ◽

Vol 53 (03) ◽

pp. 360-365 ◽

Cited By ~ 6

Author(s):

Gerhard K M Endresen ◽

Øystein Førre

Keyword(s):

Platelet Aggregation ◽

Lupus Erythematosus ◽

Immune Complexes ◽

Immunofluorescent Staining ◽

Induce Platelet Aggregation ◽

Platelet Surface ◽

Platelet Aggregometry ◽

Surface Staining ◽

Alpha1 Antitrypsin ◽

Induced Aggregation

SummarySeveral antibody fractions and sera from patients with rheumatoid arthritis, systemic lupus erythematosus and chronic idiopathic thrombocytopenic purpura were examined for their ability to bind to normal platelets using immunofluorescent staining techniques. Platelet aggregometry was used to study the activating capacity of the samples.Both C1q, C1s, C1 inactivator, fibrinogen, factor VIII-related antigen, alpha1-acid glycoprotein, alpha1-antitrypsin, beta2-micro- globulin and isoantigens A and B, as well as fibronectin and plasminogen were found on the platelet surface. Only antibodies to C1q, C1s and beta2-microglobulin were able to induce platelet aggregation. Sera containing immune complexes or platelet autoantibodies revealed positive surface staining for IgG, or for IgG and IgM. These sera also induced aggregation of platelets. Sera not containing immune complexes or autoantibodies gave negative staining and aggregation results. Thus, only some of the ligand receptor interactions were able to induce platelet aggregation.

Download Full-text