scholarly journals In Vivo NADH Fluorescence Imaging Indicates Effect of Aquaporin-4 Deletion on Oxygen Microdistribution in Cortical Spreading Depression

2013 ◽  
Vol 33 (7) ◽  
pp. 996-999 ◽  
Author(s):  
Alexander S Thrane ◽  
Takahiro Takano ◽  
Vinita Rangroo Thrane ◽  
Fushun Wang ◽  
Weiguo Peng ◽  
...  
Keyword(s):  
Oxygen Consumption ◽  
Fluorescence Imaging ◽  
Nicotinamide Adenine Dinucleotide ◽  
Oxygen Delivery ◽  
Cortical Spreading Depression ◽  
Spreading Depression ◽  
Aquaporin 4 ◽  
Two Photon ◽  
Nadh Fluorescence

Using in vivo two-photon imaging, we show that mice deficient in aquaporin-4 (AQP4) display increased fluorescence of nicotinamide adenine dinucleotide (NADH) when subjected to cortical spreading depression. The increased NADH signal, a proxy of tissue hypoxia, was restricted to microwatershed areas remote from the vasculature. Aqp4 deletion had no effects on the hyperemia response, but slowed [K+]o recovery. These observations suggest that K+ uptake is suppressed in Aqp4−/- mice as a consequence of decreased oxygen delivery to tissue located furthest away from the vascular source of oxygen, although increased oxygen consumption may also contribute to our observations.

scholarly journals In Vivo Uptake of [3H]Nimodipine into Brain during Cortical Spreading Depression

1997 ◽  
Vol 17 (5) ◽  
pp. 586-590 ◽  
Author(s):  
Sachiko Osuga ◽  
Antoine M. Hakim ◽  
Hitoshi Osuga ◽  
Matthew J. Hogan
Keyword(s):  
Rat Brain ◽  
Cortical Spreading Depression ◽  
Spreading Depression ◽  
Control Group ◽  
Similar Measurement ◽  
Contralateral Hemisphere ◽  
Radiotracer Uptake ◽  
Uptake Studies ◽  
In Vivo Uptake

We report autoradiographic measurements of the in vivo uptake of [3H]nimodipine during the nonischemic depolarization of cortical spreading depression (CSD) in rat brain. [3H]Nimodipine uptake in brain was determined regionally in rats undergoing CSD (n = 8) and was significantly increased in cortex (14 ± 7%) and hippocampus (10 ± 6%) on the stimulated side relative to the contralateral hemisphere when compared with the same measurements in a control group (n = 8). A similar measurement using the physiologically inert radiotracer [14C]iodoantipyrine to control for potential effects of CSD on radioligand distribution showed a minimal increase (2.4 ± 0.7%) of radiotracer uptake in cortex after CSD. This increase was significantly less than that observed in the [3H]nimodipine uptake studies. We hypothesize that increased in vivo [3H]nimodipine uptake in CSD identifies regions of depolarization and thus infers activation of the L-type voltage sensitive calcium channels.

Diffusion-Weighted MRI Used to Detect in Vivo Modulation of Cortical Spreading Depression: Comparison of Sumatriptan and Tonabersat

2001 ◽  
Vol 172 (2) ◽  
pp. 342-353 ◽  
Author(s):  
Daniel P. Bradley ◽  
Martin I. Smith ◽  
Chaiyapoj Netsiri ◽  
Justin M. Smith ◽  
Kurt H.J. Bockhorst ◽  
...  
Keyword(s):  
Cortical Spreading Depression ◽  
Spreading Depression ◽  
Diffusion Weighted Mri ◽  
Diffusion Weighted

scholarly journals Aquaporin‐4‐independent volume dynamics of astroglial endfeet during cortical spreading depression

Glia ◽  
2019 ◽  
Vol 67 (6) ◽  
pp. 1113-1121 ◽  
Author(s):  
Brana Rosic ◽  
Didrik B. Dukefoss ◽  
Knut Sindre Åbjørsbråten ◽  
Wannan Tang ◽  
Vidar Jensen ◽  
...  
Keyword(s):  
Cortical Spreading Depression ◽  
Spreading Depression ◽  
Aquaporin 4

scholarly journals Dynamic diameter response of intraparenchymal penetrating arteries during cortical spreading depression and elimination of vasoreactivity to hypercapnia in anesthetized mice

2016 ◽  
Vol 37 (2) ◽  
pp. 657-670 ◽  
Author(s):  
Miyuki Unekawa ◽  
Yutaka Tomita ◽  
Kazuto Masamoto ◽  
Haruki Toriumi ◽  
Takashi Osada ◽  
...  
Keyword(s):  
Blood Flow ◽  
Cortical Spreading Depression ◽  
Regional Cerebral Blood Flow ◽  
Spreading Depression ◽  
Regional Cerebral Blood ◽  
Basal Diameter ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Dynamic Diameter ◽  
Current Potential

Cortical spreading depression (CSD) induces marked hyperemia with a transient decrease of regional cerebral blood flow (rCBF), followed by sustained oligemia. To further understand the microcirculatory mechanisms associated with CSD, we examined the temporal changes of diameter of intraparenchymal penetrating arteries during CSD. In urethane-anesthetized mice, the diameter of single penetrating arteries at three depths was measured using two-photon microscopy during passage of repeated CSD, with continuous recordings of direct current potential and rCBF. The first CSD elicited marked constriction superimposed on the upstrokes of profound dilation throughout each depth of the penetrating artery, and the vasoreaction temporally corresponded to the change of rCBF. Second or later CSD elicited marked dilation with little or no constriction phase throughout each depth, and the vasodilation also temporally corresponded to the increase of rCBF. Furthermore, the peak dilation showed good negative correlations with basal diameter and increase of rCBF. Vasodilation induced by 5% CO2 inhalation was significantly suppressed after CSD passage at any depth as well as hyperperfusion. These results may indicate that CSD-induced rCBF changes mainly reflect the diametric changes of the intraparenchymal arteries, despite the elimination of responsiveness to hypercapnia.

Unprecedentedly High Tissue Penetration Capability of Co-Assembled Nanosystems for Two-Photon Fluorescence Imaging In Vivo

2015 ◽  
Vol 3 (5) ◽  
pp. 646-651 ◽  
Author(s):  
Pei-Pei Yang ◽  
Yang Yang ◽  
Yu-Juan Gao ◽  
Yi Wang ◽  
Ju-Chen Zhang ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Tissue Penetration ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Color‐Tunable Light‐up Bioorthogonal Probes for In Vivo Two‐Photon Fluorescence Imaging

2020 ◽  
Vol 26 (20) ◽  
pp. 4576-4582 ◽  
Author(s):  
Yandong Dou ◽  
Yajun Wang ◽  
Yukun Duan ◽  
Bin Liu ◽  
Qinglian Hu ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Two Photon ◽  
Color Tunable ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

A series of Zn(ii) terpyridine complexes with enhanced two-photon-excited fluorescence for in vitro and in vivo bioimaging

2015 ◽  
Vol 3 (36) ◽  
pp. 7213-7221 ◽  
Author(s):  
Qiong Zhang ◽  
Xiaohe Tian ◽  
Zhangjun Hu ◽  
Caroline Brommesson ◽  
Jieying Wu ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Cross Sections ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

TPA cross sections are enhanced for the complexes containing D–A type ligandL1.1exhibits specificity in two-photon fluorescence imaging.

scholarly journals Increased calcium influx triggers and accelerates cortical spreading depression in vivo in male adult rats

2014 ◽  
Vol 558 ◽  
pp. 87-90 ◽  
Author(s):  
Daniel Torrente ◽  
Rosângela Figueiredo Mendes-da-Silva ◽  
Andréia Albuquerque Cunha Lopes ◽  
Janneth González ◽  
George E. Barreto ◽  
...  
Keyword(s):  
Cortical Spreading Depression ◽  
Spreading Depression ◽  
Calcium Influx ◽  
Adult Rats ◽  
Male Adult
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