scholarly journals New isochaetochromin, an inhibitor of triacylglycerol synthesis in mammalian cells, produced by Penicillium sp. FKI-4942: I. Taxonomy, fermentation, isolation and biological properties

2011 ◽  
Vol 65 (1) ◽  
pp. 15-19 ◽  
Author(s):  
Narihiro Ugaki ◽  
Daisuke Matsuda ◽  
Hiroyuki Yamazaki ◽  
Kenichi Nonaka ◽  
Rokuro Masuma ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Biological Properties ◽  
Penicillium Sp ◽  
Triacylglycerol Synthesis

scholarly journals Electrospun Nanofibrous Membranes for Tissue Engineering and Cell Growth

2021 ◽  
Vol 11 (15) ◽  
pp. 6929
Author(s):  
Ewin Tanzli ◽  
Andrea Ehrmann
Keyword(s):  
Tissue Engineering ◽  
Cell Growth ◽  
Polymer Blends ◽  
Mammalian Cells ◽  
Biological Properties ◽  
Specific Substrate ◽  
Electrospun Nanofiber ◽  
Materials Used ◽  
Nanofiber Mats ◽  
Surface Morphologies

In biotechnology, the field of cell cultivation is highly relevant. Cultivated cells can be used, for example, for the development of biopharmaceuticals and in tissue engineering. Commonly, mammalian cells are grown in bioreactors, T-flasks, well plates, etc., without a specific substrate. Nanofibrous mats, however, have been reported to promote cell growth, adhesion, and proliferation. Here, we give an overview of the different attempts at cultivating mammalian cells on electrospun nanofiber mats for biotechnological and biomedical purposes. Starting with a brief overview of the different electrospinning methods, resulting in random or defined fiber orientations in the nanofiber mats, we describe the typical materials used in cell growth applications in biotechnology and tissue engineering. The influence of using different surface morphologies and polymers or polymer blends on the possible application of such nanofiber mats for tissue engineering and other biotechnological applications is discussed. Polymer blends, in particular, can often be used to reach the required combination of mechanical and biological properties, making such nanofiber mats highly suitable for tissue engineering and other biotechnological or biomedical cell growth applications.

scholarly journals E2F activity is regulated by cell cycle-dependent changes in subcellular localization.

1997 ◽  
Vol 17 (12) ◽  
pp. 7268-7282 ◽  
Author(s):  
R Verona ◽  
K Moberg ◽  
S Estes ◽  
M Starz ◽  
J P Vernon ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Subcellular Localization ◽  
Nuclear Localization ◽  
Mammalian Cells ◽  
Critical Role ◽  
Biological Properties ◽  
Dependent Manner ◽  
Restriction Point ◽  
Cycle Dependent ◽  
Almost All

E2F directs the cell cycle-dependent expression of genes that induce or regulate the cell division process. In mammalian cells, this transcriptional activity arises from the combined properties of multiple E2F-DP heterodimers. In this study, we show that the transcriptional potential of individual E2F species is dependent upon their nuclear localization. This is a constitutive property of E2F-1, -2, and -3, whereas the nuclear localization of E2F-4 is dependent upon its association with other nuclear factors. We previously showed that E2F-4 accounts for the majority of endogenous E2F species. We now show that the subcellular localization of E2F-4 is regulated in a cell cycle-dependent manner that results in the differential compartmentalization of the various E2F complexes. Consequently, in cycling cells, the majority of the p107-E2F, p130-E2F, and free E2F complexes remain in the cytoplasm. In contrast, almost all of the nuclear E2F activity is generated by pRB-E2F. This complex is present at high levels during G1 but disappears once the cells have passed the restriction point. Surprisingly, dissociation of this complex causes little increase in the levels of nuclear free E2F activity. This observation suggests that the repressive properties of the pRB-E2F complex play a critical role in establishing the temporal regulation of E2F-responsive genes. How the differential subcellular localization of pRB, p107, and p130 contributes to their different biological properties is also discussed.

scholarly journals Biophysical properties of the clinical-stage antibody landscape

2017 ◽  
Vol 114 (5) ◽  
pp. 944-949 ◽  
Author(s):  
Tushar Jain ◽  
Tingwan Sun ◽  
Stéphanie Durand ◽  
Amy Hall ◽  
Nga Rewa Houston ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Clinical Stage ◽  
Biological Properties ◽  
Therapeutic Use ◽  
Amino Acid Sequences ◽  
High Potency ◽  
Biological Drugs ◽  
Biophysical Property ◽  
Drug Candidates ◽  
Practical Guidelines

Antibodies are a highly successful class of biological drugs, with over 50 such molecules approved for therapeutic use and hundreds more currently in clinical development. Improvements in technology for the discovery and optimization of high-potency antibodies have greatly increased the chances for finding binding molecules with desired biological properties; however, achieving drug-like properties at the same time is an additional requirement that is receiving increased attention. In this work, we attempt to quantify the historical limits of acceptability for multiple biophysical metrics of “developability.” Amino acid sequences from 137 antibodies in advanced clinical stages, including 48 approved for therapeutic use, were collected and used to construct isotype-matched IgG1 antibodies, which were then expressed in mammalian cells. The resulting material for each source antibody was evaluated in a dozen biophysical property assays. The distributions of the observed metrics are used to empirically define boundaries of drug-like behavior that can represent practical guidelines for future antibody drug candidates.

scholarly journals Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations

2018 ◽  
Vol 9 ◽  
pp. 1050-1074 ◽  
Author(s):  
Jaison Jeevanandam ◽  
Ahmed Barhoum ◽  
Yen S Chan ◽  
Alain Dufresne ◽  
Michael K Danquah
Keyword(s):  
Risk Assessment ◽  
Nanostructured Materials ◽  
Mammalian Cells ◽  
Size Composition ◽  
Biological Properties ◽  
Industrial Applications ◽  
Specific Knowledge ◽  
Naturally Occurring ◽  
Physical Chemical ◽  
Toxic Reactions

Nanomaterials (NMs) have gained prominence in technological advancements due to their tunable physical, chemical and biological properties with enhanced performance over their bulk counterparts. NMs are categorized depending on their size, composition, shape, and origin. The ability to predict the unique properties of NMs increases the value of each classification. Due to increased growth of production of NMs and their industrial applications, issues relating to toxicity are inevitable. The aim of this review is to compare synthetic (engineered) and naturally occurring nanoparticles (NPs) and nanostructured materials (NSMs) to identify their nanoscale properties and to define the specific knowledge gaps related to the risk assessment of NPs and NSMs in the environment. The review presents an overview of the history and classifications of NMs and gives an overview of the various sources of NPs and NSMs, from natural to synthetic, and their toxic effects towards mammalian cells and tissue. Additionally, the types of toxic reactions associated with NPs and NSMs and the regulations implemented by different countries to reduce the associated risks are also discussed.

Citridones, New Potentiators of Antifungal Miconazole Activity, Produced by Penicillium sp. FKI-1938. Part 1. Taxonomy, Fermentation, Isolation and Biological Properties.

ChemInform ◽  
2005 ◽  
Vol 36 (47) ◽  
Author(s):  
Takashi Fukuda ◽  
Yuichi Yamaguchi ◽  
Rokuro Masuma ◽  
Hiroshi Tomoda ◽  
Satoshi Omura
Keyword(s):  
Biological Properties ◽  
Penicillium Sp

scholarly journals Comparative in vitro studies on liposomal formulations of amphotericin B and its derivative, N-methyl-N-D-fructosyl amphotericin B methyl ester (MFAME).

2002 ◽  
Vol 49 (1) ◽  
pp. 67-75 ◽  
Author(s):  
Barbara Cybulska ◽  
Karolina Kupczyk ◽  
Joanna Szlinder-Richert ◽  
Edward Borowski
Keyword(s):  
Methyl Ester ◽  
Amphotericin B ◽  
Mammalian Cells ◽  
Biological Properties ◽  
Antagonistic Effect ◽  
Molar Ratio ◽  
Selective Toxicity ◽  
Liposomal Formulations ◽  
Amphotericin B Methyl Ester

N-Methyl-N-D-fructosyl amphotericin B methyl ester (MFAME) is a semisynthetic derivative of the antifungal antibiotic amphotericin B (AMB). In contrast to the parent antibiotic, the derivative is characterised by low toxicity to mammalian cells and good solubility in water of its salts. Comparative studies on biological properties of free MFAME, AMB and their liposomal formulations were performed. To obtain liposomal forms, the antibiotics were incorporated into small unilamellar vesicles composed of dimyristoyl phosphatidylcholine (DMPC) and DMPC:cholesterol or ergosterol, 8:2 molar ratio. The effectivity of the liposomal and free forms of AMB and MFAME were compared by determination of fungistatic and fungicidal activity against Candida albicans ATCC 10261, potassium release from erythrocytes, and haemolysis. The results obtained indicate that in contrast to AMB, incorporation of MFAME into liposomes did not further improve its selective toxicity. Studies on the antagonistic effect of ergosterol and cholesterol on the antifungal activity of the antibiotics indicated that sterol interference was definitely less pronounced in the case of MFAME than in the case of AMB.

scholarly journals Anti-Inflammatory Effects of Analogues of N-Acyl Homoserine Lactones on Eukaryotic Cells

2020 ◽  
Vol 21 (24) ◽  
pp. 9448
Author(s):  
Agathe Peyrottes ◽  
Garance Coquant ◽  
Loïc Brot ◽  
Dominique Rainteau ◽  
Philippe Seksik ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Cell Model ◽  
Homoserine Lactone ◽  
Biological Properties ◽  
Host Cells ◽  
Medical Community ◽  
Eukaryotic Cells ◽  
Reporter Strain ◽  
Ifn Γ ◽  
Anti Inflammatory

Background: Since acyl-homoserine lactone (AHL) profiling has been described in the gut of healthy subjects and patients with inflammatory bowel disease (IBD), the potential effects of these molecules on host cells have raised interest in the medical community. In particular, natural AHLs such as the 3-oxo-C12-HSL exhibit anti-inflammatory properties. Our study aimed at finding stable 3-oxo-C12-HSL-derived analogues with improved anti-inflammatory effects on epithelial and immune cells. Methods: We first studied the stability and biological properties of the natural 3-oxo-C12-HSL on eukaryotic cells and a bacterial reporter strain. We then constructed and screened a library of 22 AHL-derived molecules. Anti-inflammatory effects were assessed by cytokine release in an epithelial cell model, Caco-2, and a murine macrophage cell line, RAW264.7, (respectively, IL-8 and IL-6) upon exposure to the molecule and after appropriate stimulation (respectively, TNF-α 50 ng/mL and IFN-γ 50 ng/mL, and LPS 10 ng/mL and IFN-γ 20 U/mL). Results: We found two molecules of interest with amplified anti-inflammatory effects on mammalian cells without bacterial-activating properties in the reporter strain. The molecules furthermore showed improved stability in biological medium compared to the native 3-oxo-C12-HSL. Conclusions: We provide new bio-inspired AHL analogues with strong anti-inflammatory properties that will need further study from a therapeutic perspective.

ChemInform Abstract: Dinapinones, Novel Inhibitors of Triacylglycerol Synthesis in Mammalian Cells, Produced by Penicillium pinophilum FKI-3864.

ChemInform ◽  
2011 ◽  
Vol 42 (47) ◽  
pp. no-no
Author(s):  
Satoshi Ohte ◽  
Daisuke Matsuda ◽  
Ryuji Uchida ◽  
Kenichi Nonaka ◽  
Rokuro Masuma ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Triacylglycerol Synthesis ◽  
Penicillium Pinophilum
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