Co-operative effect between insulin-like growth factor-1 and interleukin-2 on DNA synthesis and interleukin-2 receptor-α chain expression in human lymphocytes

1995 ◽  
Vol 73 (4) ◽  
pp. 340-345 ◽  
Author(s):  
ROXANA SCHILLACI ◽  
MARIANA G BROCARDO ◽  
ALICIA ROLDAN
Keyword(s):  
Growth Factor ◽  
Dna Synthesis ◽  
Human Lymphocytes ◽  
Interleukin 2 ◽  
Insulin Like Growth Factor ◽  
Interleukin 2 Receptor ◽  
Α Chain

Mutation of Asp20of human interleukin-2 reveals a dual role of the p55 α chain of the interleukin-2 receptor

1993 ◽  
Vol 23 (4) ◽  
pp. 917-921 ◽  
Author(s):  
Claudia L. Flemming ◽  
Stephen J. Russell ◽  
Mary K. L. Collins
Keyword(s):  
Interleukin 2 ◽  
Dual Role ◽  
Interleukin 2 Receptor ◽  
Α Chain

Post-transcriptional control of the onset of DNA synthesis by an insulin-like growth factor

1984 ◽  
Vol 4 (9) ◽  
pp. 1807-1814
Author(s):  
J Campisi ◽  
A B Pardee
Keyword(s):  
Cell Cycle ◽  
Growth Factors ◽  
Growth Factor ◽  
Dna Synthesis ◽  
Transcriptional Control ◽  
S Phase ◽  
G1 Phase ◽  
Insulin Like Growth Factor ◽  
Igf I ◽  
Translational Inhibition

The control of eucaryotic cell proliferation is governed largely by a series of regulatory events which occur in the G1 phase of the cell cycle. When stimulated to proliferate, quiescent (G0) 3T3 fibroblasts require transcription, rapid translation, and three growth factors for the growth state transition. We examined exponentially growing 3T3 cells to relate the requirements for G1 transit to those necessary for the transition from the G0 to the S phase. Cycling cells in the G1 phase required transcription, rapid translation, and a single growth factor (insulin-like growth factor [IGF] I) to initiate DNA synthesis. IGF I acted post-transcriptionally at a late G1 step. All cells in the G1 phase entered the S phase on schedule if either insulin (hyperphysiological concentration) or IGF I (subnanomolar concentration) was provided as the sole growth factor. In medium lacking all growth factors, only cells within 2 to 3 h of the S phase were able to initiate DNA synthesis. Similarly, cells within 2 to 3 h of the S phase were less dependent on transcription and translation for entry into the S phase. Cells responded very differently to inhibited translation than to growth factor deprivation. Cells in the early and mid-G1 phases did not progress toward the S phase during transcriptional or translational inhibition, and during translational inhibition they actually regressed from the S phase. In the absence of growth factors, however, these cells continued progressing toward the S phase, but still required IGF at a terminal step before initiating DNA synthesis. We conclude that a suboptimal condition causes cells to either progress or regress in the cell cycle rather than freezing them at their initial position. By using synchronized cultures, we also show that in contrast to earlier events, this final, IGF-dependent step did not require new transcription. This result is in contrast to findings that other growth factors induce new transcription. We examined the requirements for G1 transit by using a chemically transformed 3T3 cell line (BPA31 cells) which has lost some but not all ability to regulate its growth. Early- and mid-G1-phase BPA31 cells required transcription and translation to initiate DNA synthesis, although they did not regress from the S phase during translational inhibition. However, these cells did not need IGF for entry into the S phase.

Insulin-like Growth Factor II Induces DNA Synthesis in Fetal Ventricular Myocytes In Vitro

1996 ◽  
Vol 79 (4) ◽  
pp. 716-726 ◽  
Author(s):  
Qingquan Liu ◽  
Huajun Yan ◽  
Nicola J. Dawes ◽  
Giuliano A. Mottino ◽  
Joy S. Frank ◽  
...  
Keyword(s):  
Growth Factor ◽  
Dna Synthesis ◽  
Ventricular Myocytes ◽  
Insulin Like Growth Factor ◽  
Factor Ii

Peptide nucleic acids directed to the promoter of the α-chain of the interleukin-2 receptor

1996 ◽  
Vol 1309 (3) ◽  
pp. 226-238 ◽  
Author(s):  
Danièle Praseuth ◽  
Mikhaïl Grigoriev ◽  
Anne-Laure Guieysse ◽  
Linda Louise Pritchard ◽  
Annick Harel-Bellan ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Peptide Nucleic Acids ◽  
Interleukin 2 ◽  
Interleukin 2 Receptor ◽  
Α Chain

scholarly journals Insulin-Like Growth Factor-l Potentiates Protein Synthesis Induced by Thyrotropin in FRTL-5 Cells: Comparison of Induction of Protein Synthesis and DNA Synthesis.

1998 ◽  
Vol 45 (2) ◽  
pp. 151-163 ◽  
Author(s):  
TOMOKO KOIDE ◽  
YUKITSUGU ONO ◽  
YOSHIAKI ITO ◽  
MASAKAZU AKAHORI ◽  
TAKU NEDACHI ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Growth Factor ◽  
Dna Synthesis ◽  
Insulin Like Growth Factor

Expression of Soluble Human Interleukin-2 Receptor α-Chain inEscherichia coli

1995 ◽  
Vol 6 (6) ◽  
pp. 737-747 ◽  
Author(s):  
Stella Dracheva ◽  
Robert E. Palermo ◽  
Gordon D. Powers ◽  
David S. Waugh
Keyword(s):  
Interleukin 2 ◽  
Inescherichia Coli ◽  
Interleukin 2 Receptor ◽  
Α Chain
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