PANCREATIN-EDTA TREATMENT AFFECTS BUOYANCY OF CELLS IN COHN FRACTION V PROTEIN DENSITY GRADIENTS WITHOUT RESIDUAL EFFECT ON CELL SIZE

1983 ◽  
Vol 61 (6) ◽  
pp. 727-737 ◽  
Author(s):  
JW Sheridan ◽  
RJ Simmons
Keyword(s):  
Cell Size ◽  
Residual Effect ◽  
Density Gradients ◽  
V Protein ◽  
Edta Treatment ◽  
Protein Density

scholarly journals Calcium Cycling Protein Density and Functional Importance to Automaticity of Isolated Sinoatrial Nodal Cells Are Independent of Cell Size

2007 ◽  
Vol 100 (12) ◽  
pp. 1723-1731 ◽  
Author(s):  
Alexey E. Lyashkov ◽  
Magdalena Juhaszova ◽  
Halina Dobrzynski ◽  
Tatiana M. Vinogradova ◽  
Victor A. Maltsev ◽  
...  
Keyword(s):  
Cell Size ◽  
Calcium Cycling ◽  
Functional Importance ◽  
Protein Density

scholarly journals Nanostructured polymer brushes and protein density gradients on diamond by carbon templating

Soft Matter ◽  
2011 ◽  
Vol 7 (10) ◽  
pp. 4861 ◽  
Author(s):  
Naima A. Hutter ◽  
Marin Steenackers ◽  
Andreas Reitinger ◽  
Oliver A. Williams ◽  
Jose A. Garrido ◽  
...  
Keyword(s):  
Polymer Brushes ◽  
Density Gradients ◽  
Nanostructured Polymer ◽  
Protein Density

Protein Density Gradients on Surfaces

Langmuir ◽  
2002 ◽  
Vol 18 (7) ◽  
pp. 2463-2467 ◽  
Author(s):  
Isabelle Caelen ◽  
Hui Gao ◽  
Hans Sigrist
Keyword(s):  
Density Gradients ◽  
Protein Density

Spindle scaling mechanisms

2020 ◽  
Vol 64 (2) ◽  
pp. 383-396
Author(s):  
Lara K. Krüger ◽  
Phong T. Tran
Keyword(s):  
Cell Size ◽  
Spindle Assembly ◽  
Dependent Manner ◽  
Post Translational Modification ◽  
Size Dependent ◽  
A Cell ◽  
Chromosome Separation ◽  
Spindle Elongation ◽  
Protein Gradients ◽  
Spindle Structure

Abstract The mitotic spindle robustly scales with cell size in a plethora of different organisms. During development and throughout evolution, the spindle adjusts to cell size in metazoans and yeast in order to ensure faithful chromosome separation. Spindle adjustment to cell size occurs by the scaling of spindle length, spindle shape and the velocity of spindle assembly and elongation. Different mechanisms, depending on spindle structure and organism, account for these scaling relationships. The limited availability of critical spindle components, protein gradients, sequestration of spindle components, or post-translational modification and differential expression levels have been implicated in the regulation of spindle length and the spindle assembly/elongation velocity in a cell size-dependent manner. In this review, we will discuss the phenomenon and mechanisms of spindle length, spindle shape and spindle elongation velocity scaling with cell size.

Concanavalin A-induced Aggregation of Human Blood Platelets

1975 ◽  
Vol 33 (02) ◽  
pp. 354-360 ◽  
Author(s):  
Heinrich Patscheke ◽  
Reinhard Brossmer
Keyword(s):  
Concanavalin A ◽  
Binding Capacity ◽  
Specific Binding ◽  
Blood Platelets ◽  
Residual Effect ◽  
Independent Effect ◽  
Con A ◽  
Experimental Conditions ◽  
Main Effect ◽  
Induced Aggregation

SummaryConcanavalin A (CON A) causes platelets to aggregate. A Ca++-independent effect of CON A could be separated from a main effect which depends on Ca++. The main effect probably is a consequence of the CON A-induced platelet release reaction and therefore is platelet-specific. The weak residual effect observed in the presence of Na2EDTA may be due to a similar mechanism as has been demonstrated for CON A-induced aggregations of several other normal and malignant transformed animal cells.Na2EDTA did not inhibit the carbohydrate-specific binding capacity of CON A. Therefore, Na2EDTA appears not to demineralize the CON A molecules under these experimental conditions.α-methyl-D-glucoside inhibits the Ca++-independent as well as the Ca++-dependent effect of CON A.Pretreatment by neuraminidase stimulated the platelet aggregation induced by CON A. It is possible that removal of terminal sialic acid residues makes additional receptors accessible for the binding of CON A.

Shifts in cell size and community composition of bacterioplankton due to grazing by heterotrophic flagellates: evidence from a marine system

2019 ◽  
Vol 83 (3) ◽  
pp. 295-308
Author(s):  
MG Weinbauer ◽  
S Suominen ◽  
J Jezbera ◽  
ME Kerros ◽  
S Marro ◽  
...  
Keyword(s):  
Cell Size ◽  
Community Composition ◽  
Heterotrophic Flagellates ◽  
Marine System

Thigh Cuff Effects on Venous Flow Redistribution During 4 Days in Dry Immersion

2020 ◽  
Vol 91 (9) ◽  
pp. 697-702
Author(s):  
Philippe Arbeille ◽  
Danielle Greaves ◽  
Laurent Guillon ◽  
Stephane Besnard
Keyword(s):  
Left Ventricle ◽  
Ejection Fraction ◽  
Jugular Vein ◽  
Residual Effect ◽  
Cerebral Vein ◽  
Fluid Shift ◽  
Venous Flow ◽  
Additional Measure ◽  
Dry Immersion ◽  
Vein Diameter

PURPOSE: The objective was to quantify the venous redistribution during a 4-d dry immersion (DI) and evaluate the effect of thigh cuffs.METHODS: The study included nine control (Co) and nine subjects wearing thigh cuffs during the daytime (CU). Ultrasound measures were performed Pre-DI, on day 4 AM (D4 AM) and D4 PM: left ventricle stroke volume and ejection fraction (SV, EF), jugular vein volume (JVvol), portal vein diameter (PV), and middle cerebral vein velocity (MCVv). An additional measure of JVvol was performed on Day 1 after 2 h in DI.RESULTS: After 2 h in DI, JVvol increased significantly from Pre in both groups, but increased more in the Co compared to the CU subjects (Co: 0.27 0.15 cm3 to 0.94 0.22 cm3; CU: 0.32 0.13 cm3 to 0.64 0.32 cm3). At D4 AM, SV and EF decreased from Pre (SV: 111 23 cm3 to 93 24 cm3; EF: 0.66 0.07 to 0.62 0.07). JVvol was slightly increased (Co: 0.47 0.22 cm3 CU: 0.35 014 cm3). MCVv and PV remained unchanged from Pre-DI. No difference was found between the two groups for any of the parameters measured. From D4 AM to PM, no significant change was observed for any parameter.CONCLUSION: The results confirm that DI induces, during the first 2-3 h, a significant cephalic fluid shift as observed in spaceflight. During this early phase, the thigh cuffs reduced the amplitude of the fluid shift toward the head, but after 4 d in DI there was only a slight memory (residual) effect of DI on the jugular volume and no residual effect of the thigh cuffs.Arbeille P, Greaves D, Guillon L, Besnard S. Thigh cuff effects on venous flow redistribution during 4 days in dry immersion. Aerosp Med Hum Perform. 2020; 91(9):697702.

Nutrient economy through land configuration and residue management in a greengram (Phaseolus radiatus L.)-wheat (Triticum aestivum L.) cropping sequence with limited water supplies

2005 ◽  
Vol 52 (4) ◽  
pp. 369-379
Author(s):  
B. G. Shivakumar ◽  
B. N. Mishra ◽  
R. C. Gautam
Keyword(s):  
Grain Yield ◽  
Agricultural Research ◽  
Indian Agricultural Research Institute ◽  
Residual Effect ◽  
Residue Management ◽  
Phosphorus Fertilization ◽  
Significant Interaction Effect ◽  
Preceding Crop ◽  
Cropping Sequence ◽  
Phosphorus Application

A field experiment on a greengram-wheat cropping sequence was carried out under limited water supply conditions in 1997-98 and 1998-99 at the farm of the Indian Agricultural Research Institute, New Delhi. The greengram was sown either on flat beds or on broad beds 2 m in width, divided by furrows, with 0, 30 and 60 kg P2O5/ha. After the harvest of greengram pods, wheat was grown in the same plots, either with the greengram stover removed or with the stover incorporated along with 0, 40, 80 and 120 kg N/ha applied to wheat. The grain yield of greengram was higher when sown on broad beds with furrows compared to flat bed sowing, and the application of 30 or 60 kg P2O5/ha resulted in significantly higher grain yields compared to no phosphorus application. The combination of broad bed and furrows with phosphorus fertilization was found to be ideal for achieving higher productivity in greengram. The land configuration treatments had no impact on the productivity of wheat. The application of phosphorus to the preceding crop had a significant residual effect on the grain yield of wheat. The incorporation of greengram stover also significantly increased the grain yield of wheat. The increasing levels of N increased the grain yield of wheat significantly up to 80 kg/ha. The combination of greengram stover incorporation and 80 kg N/ha applied to wheat significantly increased the grain yield. Further, there was a significant interaction effect between the phosphorus applied to the preceding crop and N levels given to wheat on the grain yield of wheat.

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