THE EFFECT OF SALICYLATE ON THE IN VITRO ANTIGEN-ANTIBODY REACTION IN ANAPHYLAXIS

1959 ◽  
Vol 37 (1) ◽  
pp. 77-82 ◽  
Author(s):  
ER Trethewie ◽  
C Rumberg
Keyword(s):  
Antibody Reaction ◽  
Antigen Antibody

Independence of activation of the fibrinolytic system from certain immunologic systems

1959 ◽  
Vol 196 (2) ◽  
pp. 431-435 ◽  
Author(s):  
S. N. Kolmen ◽  
D. R. Celander ◽  
M. Mason Guest
Keyword(s):  
Fibrinolytic Activity ◽  
Complement Fixation ◽  
Fibrinolytic System ◽  
Complement Activity ◽  
Antibody Reaction ◽  
Antigen Antibody

Activation of the immunologic system either in vitro or in vivo results in nearly complete consumption of complement without activation of the fibrinolytic system. However complement activity decreased upon the induction of fibrinolytic activity even in the absence of an antigen-antibody reaction. Presumably this is due to proteolysis of some part of the complement complex since complement activity was found to be destroyed in the presence of small quantities of purified fibrinolysin. These observations are consistent with the conclusion that a) the components of complement and those of the fibrinolytic system are separate and discrete entities; b) that reactions involving complement fixation and activation do not directly influence the fibrinolytic system; and c) that activation of the fibrinolytic system results in decreases in complement through proteolysis of one or more of its components by the fibrinolysin which develops.

scholarly journals MECHANISM OF HISTAMINE-RELEASE INHIBITION BY NICOTINAMIDE IN IN VITRO ANTIGEN-ANTIBODY REACTION

1962 ◽  
Vol 11 (1) ◽  
pp. 54-64 ◽  
Author(s):  
HIDEMASA YAMASAKI ◽  
SABURO MURAOKA ◽  
MIE SUGIYAMA ◽  
KOITI ENDO
Keyword(s):  
Histamine Release ◽  
Antibody Reaction ◽  
Antigen Antibody

scholarly journals Leukocyte Antigen-Antibody Reaction and Lysis of Paroxysmal Nocturnal Hemoglobinuria Erythrocytes

Blood ◽  
1970 ◽  
Vol 36 (3) ◽  
pp. 334-336 ◽  
Author(s):  
GIROLAMO SIRCHIA ◽  
SOLDANO FERRONE ◽  
FRANCESCO MERCURIALI
Keyword(s):  
Paroxysmal Nocturnal Hemoglobinuria ◽  
Cell Lysis ◽  
Red Cells ◽  
Clinical Implications ◽  
Specific Antibodies ◽  
Leukocyte Antigen ◽  
Leukocyte Antigens ◽  
Antibody Reaction ◽  
Antigen Antibody

Abstract The interaction between leukocyte antigens and specific antibodies can cause lysis of the red cells of paroxysmal nocturnal hemoglobinuria (PNH) in vitro. It is suggested that the activation of complement ensuing from the antigen-antibody interaction is responsible for PNH cell lysis. The clinical implications of this finding are briefly outlined.

scholarly journals The Antigen-Antibody Reaction in Immunohistochemistry

2003 ◽  
Vol 51 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Claudio Montero
Keyword(s):  
Antigen Retrieval ◽  
Fixed Tissue ◽  
Immunohistochemical Reaction ◽  
Histochemical Methods ◽  
Antibody Reaction ◽  
Antigen Antibody

The problems of major concern in immunohistochemical practice are discussed in the following order: (a) the mechanism of the Ag-Ab reaction in fixed tissue as opposed to the in vitro reaction; (b) the chemistry of fixation and its influence on the final result of the immunohistochemical reaction; (c) the various procedures used for antigen retrieval in formaldehyde-fixed tissue; and (d) the consideration of the possible mechanism underlying heat-induced antigen retrieval. Suggestions for further work to attempt a clarification of the mechanism involved in the Ag-Ab reaction in immunohistochemistry resorting to existing histochemical methods for the demonstration of protein side groups are presented, together with some examples already published.

INHIBITION OF LIPOLYTIC ACTIVITY OF SYNTHETIC β1–24 AND β1–39 CORTICOTROPHIN BY ANTI-ACTH ANTISERUM

1966 ◽  
Vol 51 (1) ◽  
pp. 88-94 ◽  
Author(s):  
A. Villanueva ◽  
S. J. H. Ashcroft ◽  
J. P. Felber
Keyword(s):  
Guinea Pig ◽  
Lipolytic Activity ◽  
Peptide Hormones ◽  
Fat Pad ◽  
Double Antibody ◽  
Epididymal Fat ◽  
Antibody Complex ◽  
Radio Immunoassay ◽  
Antigen Antibody

ABSTRACT The synthetic ACTH peptides β1–39 and β1–24 stimulated lipolysis as determined by the rat epididymal fat pad in vitro. The stimulating effect of these peptides was diminished by prior incubation of the peptides with antibodies produced by the guinea-pig against ACTH. The stimulating effect of these hormones was also diminished by the double antibody system used in the radio-immunoassay of ACTH and other peptide hormones, in which incubation with antiserum is followed by precipitation of the antigen-antibody complex by rabbit anti-guinea-pig-γ-globulin.

scholarly journals Evaluation of Shear Horizontal Surface Acoustic Wave Biosensors Using “Layer Parameter” Obtained from Sensor Responses during Immunoreaction

Sensors ◽  
2021 ◽  
Vol 21 (14) ◽  
pp. 4924
Author(s):  
Koji Kano ◽  
Hiromi Yatsuda ◽  
Jun Kondoh
Keyword(s):  
Acoustic Wave ◽  
Surface Acoustic Wave ◽  
Horizontal Surface ◽  
Propagation Characteristics ◽  
Viscosity Change ◽  
Layer Parameter ◽  
Antibody Reaction ◽  
Antigen Antibody ◽  
Shear Horizontal ◽  
Molecular Dimensions

Shear horizontal surface acoustic wave (SH-SAW) biosensors measure the reaction of capture antibodies immobilized on the sensing surface to capture test molecules (antigens) by using the change in SH-SAW propagation characteristics. SH-SAW displacement exists not only on the SH-SAW propagating surface, but also partially penetrates the specimen liquid to a certain depth, which is determined by the liquid properties of the specimen and the operating frequency of the SH-SAW. This phenomenon is called viscosity penetration. In previous studies, the effect of viscosity penetration was not considered in the measurement of SH-SAW biosensors, and the mass or viscosity change caused by the specific binding of capture antibodies to the target antigen was mainly used for the measurement. However, by considering the effect of viscosity penetration, it was found that the antigen–antibody reaction could be measured and the detection characteristics of the biosensor could be improved. Therefore, this study aims to evaluate the detection properties of SH-SAW biosensors in the surface height direction by investigating the relationship between molecular dimensions and SH-SAW propagation characteristics, which are pseudo-changed by varying the diameter of gold nanoparticles. For the evaluation, we introduced a layer parameter defined by the ratio of the SH-SAW amplitude change to the SH-SAW velocity change caused by the antigen–antibody reaction. We found a correlation between the layer parameter and pseudo-varied molecular dimensions. The results suggest that SH-SAW does not only measure the mass and viscosity but can also measure the size of the molecule to be detected. This shows that SH-SAW biosensors can be used for advanced functionality.

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