scholarly journals Effect of short term directional selection on genetic variability: experiments with Drosophila melanogaster

Heredity ◽  
1982 ◽  
Vol 48 (1) ◽  
pp. 27-33 ◽  
Author(s):  
D A Sorensen ◽  
W G Hill
1994 ◽  
Vol 48 (2) ◽  
pp. 425-434 ◽  
Author(s):  
Michael G. Ritchie ◽  
Charalambos P. Kyriacou

2012 ◽  
Vol 8 (6) ◽  
pp. 1050-1054 ◽  
Author(s):  
H. Colinet ◽  
D. Renault

Immobilization of insects is necessary for various experimental purposes, and CO 2 exposure remains the most popular anaesthetic method in entomological research. A number of negative side effects of CO 2 anaesthesia have been reported, but CO 2 probably brings about metabolic modifications that are poorly known. In this work, we used GC/MS-based metabolic fingerprinting to assess the effect of CO 2 anaesthesia in Drosophila melanogaster adults. We analysed metabolic variation of flies submitted to acute CO 2 exposure and assessed the temporal metabolic changes during short- and long-term recovery. We found that D. melanogaster metabotypes were significantly affected by the anaesthetic treatment. Metabolic changes caused by acute CO 2 exposure were still manifested after 14 h of recovery. However, we found no evidence of metabolic alterations when a long recovery period was allowed (more than 24 h). This study points to some metabolic pathways altered during CO 2 anaesthesia (e.g. energetic metabolism). Evidence of short-term metabolic changes indicates that CO 2 anaesthesia should be used with utmost caution in physiological studies when a short recovery is allowed. In spite of this, CO 2 treatment seems to be an acceptable anaesthetic method provided that a long recovery period is allowed (more than 24 h).


Science ◽  
1960 ◽  
Vol 132 (3440) ◽  
pp. 1652-1652
Author(s):  
J. Bennett ◽  
R. L. Capek ◽  
T. R. Kallstedt ◽  
R. E. Moisand ◽  
R. Milkman

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