scholarly journals A designed curved DNA sequence remarkably enhances transgene expression from plasmid DNA in mouse liver

Gene Therapy ◽  
2011 ◽  
Vol 19 (8) ◽  
pp. 828-835 ◽  
Author(s):  
S Fukunaga ◽  
G Kanda ◽  
J Tanase ◽  
H Harashima ◽  
T Ohyama ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Plasmid Dna ◽  
Mouse Liver ◽  
Transgene Expression ◽  
Curved Dna

scholarly journals Enhanced transgene expression from chromatinized plasmid DNA in mouse liver

2013 ◽  
Vol 441 (1-2) ◽  
pp. 146-150 ◽  
Author(s):  
Hiroyuki Kamiya ◽  
Shiho Miyamoto ◽  
Hitomi Goto ◽  
Genki N. Kanda ◽  
Miwako Kobayashi ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Mouse Liver ◽  
Transgene Expression

scholarly journals Contribution of Epigenetic Modifications to the Decline in Transgene Expression from Plasmid DNA in Mouse Liver

Pharmaceutics ◽  
2015 ◽  
Vol 7 (3) ◽  
pp. 199-212 ◽  
Author(s):  
Lei Zang ◽  
Makiya Nishikawa ◽  
Mitsuru Ando ◽  
Yuki Takahashi ◽  
Yoshinobu Takakura
Keyword(s):  
Plasmid Dna ◽  
Mouse Liver ◽  
Transgene Expression ◽  
Epigenetic Modifications

scholarly journals Effects of carriers on transgene expression from plasmids containing a DNA sequence with high histone affinity

2009 ◽  
Vol 376 (1-2) ◽  
pp. 99-103 ◽  
Author(s):  
Hiroyuki Kamiya ◽  
Satoki Fukunaga ◽  
Takashi Ohyama ◽  
Hideyoshi Harashima
Keyword(s):  
Dna Sequence ◽  
Transgene Expression

scholarly journals The location of the left-handedly curved DNA sequence affects exogenous DNA expression in vivo

2007 ◽  
Vol 461 (1) ◽  
pp. 7-12 ◽  
Author(s):  
Hiroyuki Kamiya ◽  
Satoki Fukunaga ◽  
Takashi Ohyama ◽  
Hideyoshi Harashima
Keyword(s):  
Dna Sequence ◽  
Exogenous Dna ◽  
Curved Dna

scholarly journals Plasmid DNA-based Alphavirus Vaccines

Vaccines ◽  
2019 ◽  
Vol 7 (1) ◽  
pp. 29 ◽  
Author(s):  
Kenneth Lundstrom
Keyword(s):  
Tumor Cells ◽  
Plasmid Dna ◽  
Transgene Expression ◽  
Tumor Regression ◽  
Infectious Agents ◽  
Application Range ◽  
Mammalian Cell Lines ◽  
High Level ◽  
Rna Replicon

Alphaviruses have been engineered as vectors for high-level transgene expression. Originally, alphavirus-based vectors were applied as recombinant replication-deficient particles, subjected to expression studies in mammalian and non-mammalian cell lines, primary cell cultures, and in vivo. However, vector engineering has expanded the application range to plasmid DNA-based delivery and expression. Immunization studies with DNA-based alphavirus vectors have demonstrated tumor regression and protection against challenges with infectious agents and tumor cells in animal tumor models. The presence of the RNA replicon genes responsible for extensive RNA replication in the RNA/DNA layered alphavirus vectors provides superior transgene expression in comparison to conventional plasmid DNA-based expression. Immunization with alphavirus DNA vectors revealed that 1000-fold less DNA was required to elicit similar immune responses compared to conventional plasmid DNA. In addition to DNA-based delivery, immunization with recombinant alphavirus particles and RNA replicons has demonstrated efficacy in providing protection against lethal challenges by infectious agents and tumor cells.

scholarly journals Persistent transgene expression in mouse liver following in vivo gene transfer with a ΔE1/ΔE4 adenovirus vector

Gene Therapy ◽  
1997 ◽  
Vol 4 (5) ◽  
pp. 393-400 ◽  
Author(s):  
Q Wang ◽  
G Greenburg ◽  
D Bunch ◽  
D Farson ◽  
MH Finer
Keyword(s):  
Gene Transfer ◽  
Mouse Liver ◽  
Transgene Expression ◽  
Adenovirus Vector ◽  
In Vivo Gene Transfer
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