scholarly journals A functional AT/G polymorphism in the 5′-untranslated region of SETDB2 in the IgE locus on human chromosome 13q14

2015 ◽  
Vol 16 (7) ◽  
pp. 488-494 ◽  
Author(s):  
R J Holt ◽  
C Vandiedonck ◽  
S A Willis-Owen ◽  
J C Knight ◽  
W O Cookson ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Untranslated Region ◽  
Immunoglobulin E ◽  
Luciferase Assay ◽  
Luciferase Reporter ◽  
Total Serum ◽  
Transcription Analysis ◽  
Serum Ige ◽  
Severe Stage ◽  
Chromosome 13Q14

Abstract The immunoglobulin E (IgE)-associated locus on human chromosome 13q14 influencing asthma-related traits contains the genes PHF11 and SETDB2. SETDB2 is located in the same linkage disequilibrium region as PHF11 and polymorphisms within SETDB2 have been shown to associate with total serum IgE levels. In this report, we sequenced the 15 exons of SETDB2 and identified a single previously ungenotyped mutation (AT/G, rs386770867) in the 5′-untranslated region of the gene. The polymorphism was found to be significantly associated with serum IgE levels in our asthma cohort (P=0.0012). Electrophoretic mobility shift assays revealed that the transcription factor Ying Yang 1 binds to the AT allele, whereas SRY (Sex determining Region Y) binds to the G allele. Allele-specific transcription analysis (allelotyping) was performed in 35 individuals heterozygous for rs386770867 from a panel of 200 British families ascertained through probands with severe stage 3 asthma. The AT allele was found to be significantly overexpressed in these individuals (P=1.26 × 10−21). A dual-luciferase assay with the pGL3 luciferase reporter gene showed that the AT allele significantly affects transcriptional activities. Our results indicate that the IgE-associated AT/G polymorphism (rs386770867) regulates transcription of SETDB2.

scholarly journals The Study of a Possible Correlation between Serum Levels of Interleukin 17 and Clinical Severity in Patients with Allergic Rhinitis

2017 ◽  
Vol 8 (3) ◽  
pp. ar.2017.8.0207
Author(s):  
Mai Aly Gharib Aly ◽  
Mohamed Tawfik El Tabbakh ◽  
Waheed Fawzy Heissam ◽  
Said Hamed Abbadi
Keyword(s):  
Allergic Rhinitis ◽  
Immunoglobulin E ◽  
Skin Testing ◽  
Allergic Diseases ◽  
Serum Levels ◽  
Clinical Severity ◽  
Total Serum ◽  
Interleukin 17 ◽  
Serum Ige ◽  
Cluster Immunotherapy

Introduction Allergic rhinitis (AR) is one of the most common allergic diseases, which affects ~20% of the world's population. T-helper (Th) type 2 cells produce interleukin (IL) 4 and IL-13, and mediate allergic responses, and these cytokines have been extensively studied as key players in the atopic airway diseases. However, the involvement of Th17 cells and IL-17 in AR has not been clearly examined. Aim To reevaluate AR clinical severity with serum IL-17, whether IL-17 affects the disease alone or in contribution with the atopic predisposition. Patients and Methods During an 18-month period, 39 individuals were divided into three groups: A, (13 control), B (13 with mild-to-moderate AR), and C (13 with severe AR). Both group B and group C patients (26) were subjected to clinical examination and allergy skin testing, and to measurement of both total serum immunoglobulin E (IgE) and IL-17 levels. Eleven patients with AR then were exposed to 6 months of cluster immunotherapy, whereas the rest of the patients were not exposed. Results Revealed a significant elevation of serum IL-17 levels with an associated increase in serum IgE in the patients with AR compared with controls and revealed that the serum levels of both total serum IgE and IL-17 decreased significantly after cluster immunotherapy. Conclusion These preliminary results added new data about the use of injective immunotherapy as well as reported on the use of sublingual immunotherapy.

Dose-Dependent Effect of Cotinine-Verified Tobacco Smoking on Serum Immunoglobulin E Levels in Korean Adult Males

2017 ◽  
Vol 21 (6) ◽  
pp. 813-817 ◽  
Author(s):  
Jae-June Dong ◽  
Jay J Shen ◽  
Yong-Jae Lee
Keyword(s):  
Tobacco Smoking ◽  
Immunoglobulin E ◽  
Allergic Diseases ◽  
Serum Immunoglobulin ◽  
Total Serum ◽  
Adult Males ◽  
Serum Ige ◽  
Korean Adult ◽  
Dose Dependent ◽  
Dose Dependent Effect

Abstract Background Smoking is one of the risk factors to exacerbate allergic diseases, and it may affect serum immunoglobulin E (IgE) levels. However, few studies have relied on an objective biomarker to examine the effect of tobacco smoking on serum IgE levels. Method A nationwide cross-sectional study was conducted to examine the relationship between urinary cotinine (Ucot) concentrations and IgE levels in 973 males using data from the 2010 Korean National Health and Nutrition Examination Survey (KNHANES). Ucot was classified into four groups based on concentration (ng/mL) as follows: nonsmoker group (Ucot <50 ng/mL) and three tertile groups in smokers (T1 [Ucot: 50.00–921.28 ng/mL]; T2 [Ucot: 921.29–1869.36 ng/mL]; and T3 [Ucot ≥1869.37 ng/mL]). The dose-response relationships between Ucot concentrations and total serum IgE level were estimated using analysis of covariance (ANCOVA) and multiple linear regression analysis after adjusting for confounding variables. Results We found a significant and positive dose-related effect of cigarette smoking as measured by Ucot concentrations on the total serum IgE level. The multivariate adjusted means of total serum IgE levels (SE) were 321.0 (36.3), 404.4 (102.7), 499.2 (79.2), and 534.7 (82.7) IU/mL, after adjusting for age, body mass index, alcohol ingestion, physical exercise, job, and household income. The regression coefficient β for total serum IgE was β = 68.6 with increasing level of Ucot group after adjusting for the same covariables (p = .009). Conclusion These findings suggest that the amount of smoking may have a dose-dependent effect on total serum IgE levels. Implication Smoking is one of the risk factors to exacerbate allergic diseases, and it may affect serum immunoglobulin E (IgE) levels, which is closely related to type 1 mediated allergic diseases. However, few studies have relied on an objective biomarker to examine the effect of tobacco smoking on serum IgE levels. We found that tobacco exposure, as measured by Ucot concentrations, increased the serum IgE levels in a dose-response manner in a representative sample of Korean adult males.

scholarly journals Erratum: A functional AT/G polymorphism in the 5′-untranslated region of SETDB2 in the IgE locus on human chromosome 13q14

2016 ◽  
Vol 18 (1) ◽  
pp. 57-57
Author(s):  
R J Holt ◽  
C Vandiedonck ◽  
S A Willis-Owen ◽  
J C Knight ◽  
W O Cookson ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Untranslated Region ◽  
Chromosome 13Q14

scholarly journals Increased Total Serum Immunoglobulin E in Children Developing Mycoplasma pneumoniae-related Extra-pulmonary Diseases

2018 ◽  
Author(s):  
Dimitri Poddighe ◽  
Elena Virginia Comi ◽  
Ilaria Brambilla ◽  
Amelia Licari ◽  
Paola Bruni ◽  
...  
Keyword(s):  
Mycoplasma Pneumoniae ◽  
Immunoglobulin E ◽  
Respiratory Infections ◽  
Pulmonary Diseases ◽  
Serum Immunoglobulin ◽  
Total Serum ◽  
Significant Elevation ◽  
Serum Ige ◽  
Group I ◽  
Extra Pulmonary

Mycoplasma pneumoniae has been recognized to be involved in several extra-pulmonary diseases, but the underlying immunologic mechanisms are still largely unknown. Recently, we observed a significant elevation of serum Immunoglobulin E (IgE) in a small group of these children. Here, we assessed total serum IgE levels in children affected with Mycoplasma pneumoniae-related extra-pulmonary diseases. We prospectively collected the data of 162 children admitted to the hospital (because of respiratory infections or extra-pulmonary diseases) who were evaluated for Mycoplasma pneumoniae serology and total serum IgE levels, concomitantly. Based upon clinical and serology aspects, 3 groups of children were identified: I) with non-mycoplasma respiratory disease; II) with mycoplasma-related respiratory diseases; III) with extra-pulmonary diseases related to concomitant/recent Mycoplasma pneumoniae infection. Interestingly, children with Mycoplasma pneumoniae-related extra-pulmonary diseases showed a significant elevation of total serum IgE. In particular, patients developing Mycoplasma pneumoniae-related extra-pulmonary diseases (group III) showed significantly higher level of IgE than both previous groups (p<0.001 vs. group I; p<0.01 vs. group II). In conclusion, hospitalized children diagnosed with Mycoplasma pneumoniae-related extra-pulmonary diseases resulted to have significantly increased serum IgE compared to children developing respiratory illnesses only.

scholarly journals Difference in Total Serum Immunoglobulin E Level Among Patients With and Without Intravenous Contrast Media Adverse Reaction on Computed Tomography Scan Examination

2019 ◽  
Vol 2 (1) ◽  
pp. 10-14
Author(s):  
Yulikha Ikhmawati ◽  
Zuhrial Zubir ◽  
Elvita Rahmi Daulay
Keyword(s):  
Computed Tomography ◽  
Adverse Reaction ◽  
Contrast Media ◽  
Adverse Reactions ◽  
Immunoglobulin E ◽  
Total Serum ◽  
Intravenous Contrast ◽  
Cross Sectional ◽  
Serum Ige ◽  
Intravenous Contrast Media

The adverse reaction (AR) to intravenous contrast media (ICM) are relatively common. Various opinions pro-posed in the mechanism of the incidence of ICM AR. Suspected that the role of Immunoglobulin E (IgE) me-diates allergic conditions that are part of the AR. Objective:To determine the difference in total serum IgE level  among subjects with and without ICM AR on computed tomography (CT) scan examination. To ob-serve difference in total serum IgE levels in subjects undergoing ICM adverse reaction based on the degree of severity. Method: An analytical study with cross-sectional design of 104 subjects undergoing CT scans with ICM, examined IgE levels before and after ICM was administered, then measured the severity of adverse  re-actions of contrast media. Result: In this study, subjects undergoing adverse reactions, prior to administration Iopamidol  had a higher mean IgE 1270.79 mg / dl compared with those who having AR  1174.90 mg / dl and after administration of Iopamidol  a mean of Ig E is still higher in subjects having AR  1507.96 mg/dl com-pared with those having negative AR that is 1325.88 mg/dl, p = 0.696, statistically, not significant. Mild reac-tions in 40 subjects (38.5%) with cough, nausea, dizziness, itching, and sweating. Conclusion: Increase in total IgE levels in subjects with positive adverse reactions are independent(p=0.696).

scholarly journals Atopic dermatitis and its association with serum immunoglobulin E levels: our experience in KVG medical college and hospital, Karnataka

2021 ◽  
Vol 8 (1) ◽  
pp. 50
Author(s):  
Priyanka K. ◽  
Abhirup H. R. ◽  
Badrinath N. ◽  
Aishwarya K. C.
Keyword(s):  
Atopic Dermatitis ◽  
Immunoglobulin E ◽  
Age Of Onset ◽  
Seborrheic Dermatitis ◽  
Serum Immunoglobulin ◽  
Total Serum ◽  
Clinical Parameters ◽  
Cross Sectional ◽  
Serum Ige ◽  
Chronic Eczema

<p><strong>Background:</strong> Eczema is an inflammatory skin reaction which presents as acute, subacute and chronic forms. Eczemas persisting for more than 6 weeks or characterized by thickening and discoloration of skin is typical of chronic eczema. Atopic dermatitis (AD) is a type of chronic or chronically relapsing eczematous skin disorder. To determine the percentage of AD in all forms of chronic eczema by using HRC. We also estimated serum immunoglobulin E (IgE) levels and determined its correlation with chronic eczemas and with various clinical parameters of HRC.</p><p><strong>Methods:</strong> A total of 50 patients with chronic eczema meeting defined inclusion and exclusion criteria were enrolled in this cross-sectional study after taking an informed consent and approval of institutional ethical committee. All patients were subjected to a detailed history based on a questionnaire. A thorough clinical examination was done to determine all major and minor clinical parameters of HRC for AD. Blood samples were collected and AEC and total serum IgE levels were determined.</p><p><strong>Results:</strong> Most of our study patients were females (64%). Majority of males (77.7%) were farmers and majority of females (56.2%) were housewives assisting in fieldwork activities. Various causes of chronic eczema were clinically diagnosed AD (34%), chronic actinic dermatitis (8%), polymorphic light eruption (4%), airborne contact dermatitis (10%), phyto-photodermatitis (10%), chronic hand and/or foot eczema (16%) and seborrheic dermatitis (2%). Thirty-two patients (64%) satisfied HRC. Among all clinical parameters of HRC, pruritus and xerosis were the commonest in AD patients. Serum IgE level was raised in 58% of chronic eczema and 68.7% of AD patients.</p><p><strong>Conclusions:</strong> Serum IgE levels showed significant association with typical morphology and distribution of lesions, early age of onset and perifollicular accentuation.</p><h2> </h2>

Production of a monoclonal antibody specific for ovine immunoglobulin E and its application to monitor serum IgE responses to Haemonchus contortus infection

Parasitology ◽  
1997 ◽  
Vol 114 (4) ◽  
pp. 395-406 ◽  
Author(s):  
F. N. J. KOOYMAN ◽  
P. J. S. VAN KOOTEN ◽  
J. F. HUNTLEY ◽  
A. MacKELLAR ◽  
A. W. C. A. CORNELISSEN ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Haemonchus Contortus ◽  
Immunoglobulin E ◽  
Polyclonal Antibodies ◽  
Sandwich Elisa ◽  
Specific Ige ◽  
Cross Reactivity ◽  
Total Serum ◽  
Western Blots ◽  
Serum Ige

Part of the Cε3–Cε4 region of the ovine immunoglobulin E (IgE) gene (nucleotides 1111–1575) was amplified by PCR. The recombinant protein (recIgE1-2) was expressed in E. coli and both monoclonal and polyclonal antibodies were produced. These antibodies recognized recIgE1-2 and native IgE on Western blots and in ELISA. The polyclonal serum showed cross-reactivity with other sheep immunoglobulin classes. The monoclonal antibody was specific for ovine IgE and goat IgE. Infection of sheep with the abomasal nematode Haemonchus contortus resulted in elevated IgE levels in serum 2–4 weeks after infection, as measured by sandwich ELISA using the rabbit polyclonal as capture antibody and the monoclonal antibody against ovine IgE as second antibody. A negative correlation between worm counts and total serum IgE levels at the end of the experiment was found in repeatedly infected sheep. Significant increased levels of excretory–secretory antigens specific IgE levels were found after H. contortus infection. In contrast, no significant changes in 3rd-stage larvae (L3) antigen-specific IgE titre in sera could be detected after infection.

scholarly journals DNA Methylation at Birth is Associated with Childhood Serum Immunoglobulin E Levels

2021 ◽  
Vol 14 ◽  
pp. 251686572110081
Author(s):  
Luhang Han ◽  
Akhilesh Kaushal ◽  
Hongmei Zhang ◽  
Latha Kadalayil ◽  
Jiasong Duan ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Gene Ontology ◽  
Cord Blood ◽  
Early Life ◽  
Immunoglobulin E ◽  
Allergic Diseases ◽  
Total Serum ◽  
Gene Ontology Analysis ◽  
Serum Ige ◽  
Cpg Sites

Immunoglobulin E (IgE) is known to play an important role in allergic diseases. Epigenetic traits acquired due to modification of deoxyribonucleic acid (DNA) methylation (DNAm) in early life may have phenotypic consequences through their role in transcriptional regulation with relevance to the developmental origins of diseases including allergy. However, epigenome-scale studies on the longitudinal association of cord blood DNAm with IgE over time are lacking. Our study aimed to examine the association of DNAm at birth with childhood serum IgE levels during early life. Genome-scale DNAm and total serum IgE measured at birth, 5, 8, and 11 years of children in the Taiwan Maternal and Infant Cohort Study were included in the study in the discovery stage. Linear mixed models were implemented to assess the association between cord blood DNAm at ~310K 5′-cytosine-phosphate-guanine-3′ (CpG) sites with repeated IgE measurements, adjusting for cord blood IgE. Identified statistically significant CpGs (at a false discovery rate, FDR, of 0.05) were further tested in an independent replication cohort, the Isle of Wight (IoW) birth cohort. We mapped replicated CpGs to genes and conducted gene ontology analysis using ToppFun to identify significantly enriched pathways and biological processes of the genes. Cord blood DNAm of 273 CpG sites were significantly (FDR = 0.05) associated with IgE levels longitudinally. Among the identified CpGs available in both cohorts (184 CpGs), 92 CpGs (50%) were replicated in the IoW in terms of consistency in direction of associations between DNA methylation and IgE levels later in life, and 16 of the 92 CpGs showed statistically significant associations ( P < .05). Gene ontology analysis identified 4 pathways (FDR = 0.05). The identified 16 CpG sites had the potential to serve as epigenetic markers associated with later IgE production, beneficial to allergic disease prevention and intervention.

scholarly journals Correlation of Increased Total Serum Immunoglobulin E Levels and Hidradenitis Suppurativa

2021 ◽  
Vol 21 (1) ◽  
pp. 9-12
Author(s):  
A Ballova ◽  
K Vorcakova ◽  
J Pec
Keyword(s):  
Cigarette Smoking ◽  
Hidradenitis Suppurativa ◽  
Immunoglobulin E ◽  
Serum Levels ◽  
Total Serum ◽  
Control Group ◽  
Dermatology Department ◽  
Total Ige ◽  
Severe Stage ◽  
Number Of Patients

Abstract Introduction: Hidradenitis suppurativa is a chronic inflammatory skin disease with a typical formation of inflamed nodules, abscesses, and sinus tracts usually in the axillary, inguinal, and anogenital region. We decided to investigate the possible association of hidradenitis suppurativa and total IgE elevation and to explore the patients’ characteristics which can be related to high IgE levels. Methods: We performed a retrospective observational study which included 67 patients with moderate-to-severe stage of hidradenitis suppurativa followed up in our outpatient dermatology department. Total IgE, IgA, IgG, IgM, and CRP serum levels were measured. A personal and family history was taken. We asked them about allergic diseases and cigarette smoking and determined the basic parameters such as the weight and height of the patients. Results: Elevated total IgE levels were noticed in 21 patients (31.3%), of which 6 had a history of allergic disorder. Three of them had allergic rhinoconjunctivitis and the other three were suffering from atopic dermatitis. The mean total IgE level was 203.0 IU/ml with a maximum value of 1,954 IU/ml. Analysis of the factors, such as cigarette smoking, sex, elevated CRP, body mass index, and the number of affected areas, did not show an association with increased IgE levels. Conclusion: Our study showed a higher mean value of total IgE in patients with moderate to severe hidradenitis suppurativa than in the general population. However, we did not confirm an association with any characteristics of the patients. Limitations of this work include a small number of patients and a lack of the control group, therefore further and more extensive studies are needed to support these results.

scholarly journals Total serum IgE levels in soil-transmitted helminth infected children with atopy symptoms

2014 ◽  
Vol 54 (3) ◽  
pp. 149
Author(s):  
Hendri Wijaya ◽  
Lily Irsa ◽  
Supriatmo Supriatmo ◽  
Sjabaroeddin Loebis ◽  
Rita Evalina
Keyword(s):  
Immunoglobulin E ◽  
Positive Association ◽  
Serum Immunoglobulin ◽  
Total Serum ◽  
Significant Positive Association ◽  
Cross Sectional ◽  
Serum Ige ◽  
Asthma Symptoms ◽  
High Prevalence ◽  
Soil Transmitted Helminthiasis

Background High total serum immunoglobulin E (IgE) levelsand eosinophilia are markers for atopy, but other factors mayalso play a key role in affecting atopy, such as intestinal parasiticinfection. Helminthiasis has been associated with a reduced riskof atopy and asthma symptoms in areas with high prevalence ofparasitic infections.Objective To assess for associations between total serum IgElevel and soil-transmitted helminthiasis (STH) infection, as wellas between STH infection and allergy symptoms.Methods We conducted a cross-sectional stu dy on 84consecutively-enrolled children , aged 7- 13 years, in theSecanggang Subdistrict, Langkat District, North SumateraProvince. Subjects were enrolled into one of two groups, with orwithout STH infection. Total serum IgE measurements and stoolSTH examinations were performed in all subjects. Data on atopyor allergy symptoms were obtained by parental interview.Results The 42 subjects with STH infection had significantlyhigher mean total serum lg E levels than the 42 subjects withoutinfec tion, 1,13 1.26 IU/mL and 744.76 IU/mL, respectiv ely(P = 0.029). We also fo und significant positive association sbetween STH infection and asthma symptoms (P= 0.049), as wellas eczema symptoms (P=0.044).Conclusion Mean total serum IgE level is higher in STHinfectedsubjects than in those without infection. In addition,STH infection is positively associated with asthma and eczemasymptoms.

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