scholarly journals Loss of corneal Langerhans cells during storage in organ culture medium, Optisol and McCarey-Kaufman medium

Eye ◽  
1998 ◽  
Vol 12 (1) ◽  
pp. 134-138 ◽  
Author(s):  
Navid Ardjomand ◽  
Andrea Berghold ◽  
Margarete E Reich
Keyword(s):  
Organ Culture ◽  
Langerhans Cells ◽  
Culture Medium ◽  
Organ Culture Medium

Corneal Langerhans cells during storage in organ culture medium

1997 ◽  
Vol 94 (10) ◽  
pp. 703-706 ◽  
Author(s):  
Navid Ardjomand ◽  
Peter Komericki ◽  
Herbert Radner ◽  
Reingard Aigner ◽  
Margarete Eva Reich
Keyword(s):  
Organ Culture ◽  
Langerhans Cells ◽  
Culture Medium ◽  
Organ Culture Medium

Supplementation of organ culture medium with dextran is not required in pre-stripped human donor tissue for DMEK surgery

2019 ◽  
Vol 20 (2) ◽  
pp. 193-200 ◽  
Author(s):  
Sabine Salla ◽  
Friedrich E. Kruse ◽  
Peter Walter ◽  
Johannes Menzel-Severing
Keyword(s):  
Organ Culture ◽  
Culture Medium ◽  
Human Donor ◽  
Donor Tissue ◽  
Organ Culture Medium

Swelling tendency of rabbit cornea in organ culture medium

2009 ◽  
Vol 66 (4) ◽  
pp. 369-375 ◽  
Author(s):  
Walther Medin ◽  
Martin Davanger
Keyword(s):  
Organ Culture ◽  
Culture Medium ◽  
Rabbit Cornea ◽  
Organ Culture Medium

In vitro development of theDrosophila chorion in a chemically defined organ culture medium

1979 ◽  
Vol 186 (4) ◽  
pp. 351-362 ◽  
Author(s):  
William H. Petri ◽  
Mihalis N. Mindrinos ◽  
Mary F. Lombard ◽  
Lukas H. Margaritis
Keyword(s):  
Organ Culture ◽  
Culture Medium ◽  
In Vitro Development ◽  
Organ Culture Medium ◽  
Vitro Development

scholarly journals Preservation of Preloaded DMEK Lenticules in Dextran and Non-Dextran-Based Organ Culture Medium

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Mohit Parekh ◽  
Alessandro Ruzza ◽  
Stefano Ferrari ◽  
Diego Ponzin
Keyword(s):  
Organ Culture ◽  
Glucose Uptake ◽  
Culture System ◽  
Culture Medium ◽  
Organ Culture System ◽  
Before And After ◽  
Pas Staining ◽  
Organ Culture Medium ◽  
Preservation Conditions ◽  
Histological Staining

Purpose.To determine the optimum preservation conditions for preloading DMEK lenticules using organ culture system.Methods.8.5 mm DMEK lenticules were stripped and preserved with endothelium flap-in for 4 days at RT in an IOL cartridge that was blocked with rubber stoppers from each end. In C1, tissues were collected from tissue culture medium (TCM) and preserved in TCM. In C2, tissues were collected from transport medium (TCM + 6% dextran T500) (TM) and preserved in TM. In C3, tissues were collected from TCM and preserved in TM. Mortality, glucose uptake, histological staining, tight junctions and cell apoptosis were studied post-preservation.Results.Mortality in C1, C2, and C3 were 49.40%, 8.53%, and 27.74%, with 40.7%, 13%, and 41.8% uncovered areas. Glucose uptake (mg/mL) was 0.32, 0.43, and 0.56 in C1, C2, and C3. PAS staining showed presence of DM and endothelium in C2 but not in C1 and with fewer cells in C3. ZO-1 was expressed in all the conditions. Polymorphism was higher in C1 and C3. Mild apoptosis was observed in C3.Conclusions.Dextran may play an important role in preserving the endothelial cells before and after stripping for trifolded (endothelium-in) preloaded DMEK lenticules.

Thickness and Curvature Changes of Human Corneal Grafts in Dextran-Containing Organ Culture Medium Before Keratoplasty

Cornea ◽  
2020 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Loïc Hamon ◽  
Loay Daas ◽  
Stephanie Mäurer ◽  
Isabel Weinstein ◽  
Adrien Quintin ◽  
...  
Keyword(s):  
Organ Culture ◽  
Culture Medium ◽  
Organ Culture Medium

scholarly journals Increased sensitivity of microbiological testing of cornea organ culture medium by additional resin treatment

2018 ◽  
Vol 3 (1) ◽  
pp. e000173 ◽  
Author(s):  
Zemra Skenderi ◽  
Laura Giurgola ◽  
Claudio Gatto ◽  
Jana D’Amato Tóthová ◽  
Axel Pruß ◽  
...  
Keyword(s):  
Blood Culture ◽  
Organ Culture ◽  
Culture Medium ◽  
Penicillin G ◽  
Significant Delay ◽  
Aspergillus Brasiliensis ◽  
Microbiological Testing ◽  
Group A ◽  
Organ Culture Medium ◽  
Group B

ObjectiveThis validation study investigates the treatment of cornea organ culture medium (Modified Eagle Medium, Biochrom GmbH, Berlin, Germany) with RESEP, a new medical device for antibiotics removal, before microbiological testing with BACTEC TM blood culture bottles.Methods and analysis10–100 colony forming units of Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Bacillus subtillis, Aspergillus brasiliensis, Clostridium sporogenes, Enterobacter cloacae and Staphylococcus epidermidis were inoculated in 9mL of cornea organ culture medium. In group A, the medium was withdrawn with RESEP and treated for 20 min at room temperature, and then inoculated in BACTEC Plus Aerobic/F/Anaerobic/F blood culture bottles. In group B, the medium, spiked by the inoculation of microorganism, was injected directly. For each strain, a growth control was performed, by direct inoculation of the microorganisms in BACTECTMvials (positive control). All samples were incubated in the automated BACTECTMblood culture system at 36°C ±1°C for maximum of 14 days or until a positive reading. The elimination of antibiotics from the medium by RESEP was determined by high-performance liqiud chromatography.ResultsAfter 20 min of RESEP treatment, 100% (n=9) of streptomycin, 100% (n=9) of amphotericin B and 99.7% (n=9) of penicillin G were eliminated. In group A , all microorganisms were detected within 3 days of incubation with a sensitivity of 100% (n=99) and showed no significant delay compared with the positive controls. In group B, the overall sensitivity was 67.9% (n=96) with a significant delay until detection of microbial growth for all tested microorganisms except for A. brasiliensis.ConlclusionThe use of RESEP to eliminate the antibiotics from cornea organ culture medium increases the sensitivity of the microbiological testing with BACTECTMPlus blood culture bottles significantly and fulfils the requirements of the European Pharmacopoeia method suitability test.

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