scholarly journals Grape extracts suppress the formation of preneoplastic foci and activity of fatty acid synthase in rat liver

2003 ◽  
Vol 35 (5) ◽  
pp. 371-378 ◽  
Author(s):  
Sanghui Kweon ◽  
Yoon Kim ◽  
Haymie Choi
Keyword(s):  
Fatty Acid ◽  
Rat Liver ◽  
Fatty Acid Synthase ◽  
Preneoplastic Foci

scholarly journals Transient induction of fatty acid synthase in rat liver after removal of a peroxisome proliferator

FEBS Letters ◽  
1994 ◽  
Vol 356 (1) ◽  
pp. 122-124 ◽  
Author(s):  
Kiyoto Motojima ◽  
Masanori Isaka ◽  
Yasuko Takino ◽  
Sataro Goto
Keyword(s):  
Fatty Acid ◽  
Rat Liver ◽  
Fatty Acid Synthase ◽  
Peroxisome Proliferator

Nutritional Control of Rat Liver Fatty Acid Synthase and S14 mRNA Abundance

1990 ◽  
Vol 120 (2) ◽  
pp. 218-224 ◽  
Author(s):  
Steven D. Clarke ◽  
Michael K. Armstrong ◽  
Donald B. Jump
Keyword(s):  
Fatty Acid ◽  
Rat Liver ◽  
Fatty Acid Synthase ◽  
Mrna Abundance ◽  
Liver Fatty Acid

Regulation of fatty acid synthase at transcriptional and post-transcriptional levels in rat liver

1992 ◽  
Vol 33 (3) ◽  
pp. 199 ◽  
Author(s):  
Kyung Sup Kim ◽  
Sahng Wook Park ◽  
Yoon Soo Kim
Keyword(s):  
Fatty Acid ◽  
Rat Liver ◽  
Fatty Acid Synthase

scholarly journals Effects of nutrients and hormones on transcriptional and post-transcriptional regulation of fatty acid synthase in rat liver

1990 ◽  
Vol 190 (2) ◽  
pp. 427-433 ◽  
Author(s):  
Akihiko KATSURADA ◽  
Nobuko IRITANI ◽  
Hitomi FUKUDA ◽  
Yohko MATSUMURA ◽  
Naomi NISHIMOTO ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Fatty Acid ◽  
Rat Liver ◽  
Fatty Acid Synthase ◽  
Post Transcriptional Regulation

Dietary Polyunsaturated Fats Uniquely Suppress Rat Liver Fatty Acid Synthase and S14 mRNA Content

1990 ◽  
Vol 120 (2) ◽  
pp. 225-231 ◽  
Author(s):  
Steven D. Clarke ◽  
Michael K. Armstrong ◽  
Donald B. Jump
Keyword(s):  
Fatty Acid ◽  
Rat Liver ◽  
Fatty Acid Synthase ◽  
Liver Fatty Acid ◽  
Mrna Content ◽  
Polyunsaturated Fats

scholarly journals Characterization of the inactivation of rat fatty acid synthase by C75: inhibition of partial reactions and protection by substrates

2005 ◽  
Vol 388 (3) ◽  
pp. 895-903 ◽  
Author(s):  
Alan R. RENDINA ◽  
Dong CHENG
Keyword(s):  
Fatty Acid ◽  
Rat Liver ◽  
Room Temperature ◽  
Fatty Acid Synthase ◽  
Enzyme Inhibitor ◽  
Acetyl Coa ◽  
Enoyl Reductase ◽  
Malonyl Coa ◽  
Ketoacyl Synthase

C75, a synthetic inhibitor of FAS (fatty acid synthase), has both anti-tumour and anti-obesity properties. In this study we provide a detailed kinetic characterization of the mechanism of in vitro inhibition of rat liver FAS. At room temperature, C75 is a competitive irreversible inhibitor of the overall reaction with regard to all three substrates, i.e. acetyl-CoA, malonyl-CoA and NADPH, exhibiting pseudo-first-order kinetics of the complexing type, i.e. a weak non-covalent enzyme–inhibitor complex is formed before irreversible enzyme modification. C75 is a relatively inefficient inactivator of FAS, with a maximal rate of inactivation of 1 min−1 and an extrapolated KI (dissociation constant for the initial complex) of approx. 16 mM. The apparent second-order rate constants calculated from these values are 0.06 mM−1·min−1 at room temperature and 0.21 mM−1·min−1 at 37 °C. We also provide experimental evidence that C75 inactivates the β-ketoacyl synthase (3-oxoacyl synthase) partial activity of FAS. Unexpectedly, C75 also inactivates the enoyl reductase and thioesterase partial activities of FAS with about the same rates as for inactivation of the β-ketoacyl synthase. In contrast with the overall reaction, the β-ketoacyl synthase activity and the enoyl reductase activity, substrates do not protect the thioesterase activity of rat liver FAS from inactivation by C75. These results differentiate inactivation by C75 from that by cerulenin, which only inactivates the β-ketoacyl synthase activity of FAS, by forming an adduct with an active-site cysteine. Interference by dithiothreitol and protection by the substrates, acetyl-CoA, malonyl-CoA and NADPH, further distinguish the mechanism of C75-mediated inactivation from that of cerulenin. The most likely explanation for the multiple effects observed with C75 on rat liver FAS and its partial reactions is that there are multiple sites of interaction between C75 and FAS.

scholarly journals Involvement of a Unique Carbohydrate-responsive Factor in the Glucose Regulation of Rat Liver Fatty-acid Synthase Gene Transcription

2001 ◽  
Vol 276 (24) ◽  
pp. 21969-21975 ◽  
Author(s):  
Caterina Rufo ◽  
Margarita Teran-Garcia ◽  
Manabu T. Nakamura ◽  
Seung-Hoi Koo ◽  
Howard C. Towle ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Gene Transcription ◽  
Rat Liver ◽  
Fatty Acid Synthase ◽  
Glucose Regulation ◽  
Liver Fatty Acid ◽  
Fatty Acid Synthase Gene
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