Effect of chronic alcohol feeding with a low-fat diet on acetyl CoA carboxylase and fatty acid synthase activities in rat liver

1989 ◽  
Vol 17 (6) ◽  
pp. 1116-1116 ◽  
Author(s):  
K. J. SIMPSON ◽  
S. VENKATESAN ◽  
T. J. PETERS
Keyword(s):  
Fatty Acid ◽  
Rat Liver ◽  
Fatty Acid Synthase ◽  
Chronic Alcohol ◽  
Acetyl Coa Carboxylase ◽  
Low Fat ◽  
Acetyl Coa ◽  
Low Fat Diet

scholarly journals RNA-seq reveals novel mechanistic targets of withaferin A in prostate cancer cells

2020 ◽  
Vol 41 (6) ◽  
pp. 778-789 ◽  
Author(s):  
Su-Hyeong Kim ◽  
Eun-Ryeong Hahm ◽  
Krishna B Singh ◽  
Sruti Shiva ◽  
Jacob Stewart-Ornstein ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Fatty Acid ◽  
Fatty Acid Synthase ◽  
Acid Synthesis ◽  
Acetyl Coa Carboxylase ◽  
Rna Seq ◽  
Atp Citrate Lyase ◽  
Acetyl Coa ◽  
Citrate Lyase

Abstract Withaferin A (WA) is a promising phytochemical exhibiting in vitro and in vivo anticancer activities against prostate and other cancers, but the mechanism of its action is not fully understood. In this study, we performed RNA-seq analysis using 22Rv1 human prostate cancer cell line to identify mechanistic targets of WA. Kyoto Encyclopedia of Genes and Genomes pathway analysis of the differentially expressed genes showed most significant enrichment of genes associated with metabolism. These results were validated using LNCaP and 22Rv1 human prostate cancer cells and Hi-Myc transgenic mice as models. The intracellular levels of acetyl-CoA, total free fatty acids and neutral lipids were decreased significantly following WA treatment in both cells, which was accompanied by downregulation of mRNA (confirmed by quantitative reverse transcription-polymerase chain reaction) and protein levels of key fatty acid synthesis enzymes, including ATP citrate lyase, acetyl-CoA carboxylase 1, fatty acid synthase and carnitine palmitoyltransferase 1A. Ectopic expression of c-Myc, but not constitutively active Akt, conferred a marked protection against WA-mediated suppression of acetyl-CoA carboxylase 1 and fatty acid synthase protein expression, and clonogenic cell survival. WA was a superior inhibitor of cell proliferation and fatty acid synthesis in comparison with known modulators of fatty acid metabolism including cerulenin and etomoxir. Intraperitoneal WA administration to Hi-Myc transgenic mice (0.1 mg/mouse, three times/week for 5 weeks) also resulted in a significant decrease in circulating levels of total free fatty acids and phospholipids, and expression of ATP citrate lyase, acetyl-CoA carboxylase 1, fatty acid synthase and carnitine palmitoyltransferase 1A proteins in the prostate in vivo.

scholarly journals Fatty Acid Synthase and Acetyl-CoA Carboxylase Are Expressed in Nodal Metastatic Melanoma But Not in Benign Intracapsular Nodal Nevi

2018 ◽  
Vol 40 (4) ◽  
pp. 259-264 ◽  
Author(s):  
Jad Saab ◽  
Maria Laureana Santos-Zabala ◽  
Massimo Loda ◽  
Edward C. Stack ◽  
Travis J. Hollmann
Keyword(s):  
Fatty Acid ◽  
Metastatic Melanoma ◽  
Fatty Acid Synthase ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa
Sign in / Sign up

Export Citation Format

Share Document