scholarly journals Characterization of regulatory elements on the promoter region of human ATP-citrate lyase

1999 ◽  
Vol 31 (2) ◽  
pp. 108-114 ◽  
Author(s):  
Young-Ah Moon ◽  
Kyung-Sup Kim ◽  
Un-Hyung Cho ◽  
Do-Jun Yoon ◽  
Sahng-Wook Park
Keyword(s):  
Promoter Region ◽  
Regulatory Elements ◽  
Atp Citrate Lyase ◽  
Citrate Lyase

scholarly journals Characterization of cis-acting elements in the rat ATP citrate-lyase gene promoter

2002 ◽  
Vol 34 (1) ◽  
pp. 60-68 ◽  
Author(s):  
Young-An Moon ◽  
Sahng-Wook Park ◽  
Kyung-Sup Kim
Keyword(s):  
Gene Promoter ◽  
Atp Citrate Lyase ◽  
Cis Acting ◽  
Citrate Lyase

scholarly journals Characterization of Hepatic-specific Regulatory Elements in the Promoter Region of the Human Cholesterol 7α-Hydroxylase Gene

1997 ◽  
Vol 272 (6) ◽  
pp. 3444-3452 ◽  
Author(s):  
Allen D. Cooper ◽  
Jean Chen ◽  
Mary Jane Botelho-Yetkinler ◽  
Yicheng Cao ◽  
Takahiro Taniguchi ◽  
...  
Keyword(s):  
Promoter Region ◽  
Regulatory Elements ◽  
Hydroxylase Gene

scholarly journals Functional characterization of Lilium lancifolium cold-responsive Zinc Finger Homeodomain (ZFHD) gene in abscisic acid and osmotic stress tolerance

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11508
Author(s):  
Yubing Yong ◽  
Yue Zhang ◽  
Yingmin Lyu
Keyword(s):  
Abscisic Acid ◽  
Transgenic Plants ◽  
Zinc Finger ◽  
Promoter Region ◽  
Salt Water ◽  
Functional Characterization ◽  
Regulatory Elements ◽  
Response Analysis ◽  
Lilium Lancifolium

Background. We have previously performed an analysis of the cold-responsive transcriptome in the mature leaves of tiger lily (Lilium lancifolium) by gene co-expression network identification. The results has revealed that a ZFHD gene, notated as encoding zinc finger homeodomain protein, may play an essential regulating role in tiger lily response to cold stress. Methods. A further investigation of the ZFHD gene (termed as LlZFHD4) responding to osmotic stresses, including cold, salt, water stresses, and abscisic acid (ABA) was performed in this study. Based on the transcriptome sequences, the coding region and 5′ promoter region of LlZFHD4 were cloned from mature tiger lily leaves. Stress response analysis was performed under continuous 4 °C, NaCl, PEG, and ABA treatments. Functional characterization of LlZFHD4 was conducted in transgenic Arabidopsis, tobacco, and yeast. Results. LlZFHD4 encodes a nuclear-localized protein consisting of 180 amino acids. The N-terminal region of LlZFHD4 has transcriptional activation activity in yeast. The 4 °C, NaCl, PEG, and ABA treatments induced the expression of LlZFHD4. Several stress- or hormone-responsive cis-acting regulatory elements (T-Box, BoxI. and ARF) and binding sites of transcription factors (MYC, DRE and W-box) were found in the core promoter region (789 bp) of LlZFHD4. Also, the GUS gene driven by LlZFHD4 promoter was up-regulated by cold, NaCl, water stresses, and ABA in Arabidopsis. Overexpression of LlZFHD4 improved cold and drought tolerance in transgenic Arabidopsis; higher survival rate and better osmotic adjustment capacity were observed in LlZFHD4 transgenic plants compared to wild type (WT) plants under 4 °C and PEG conditions. However, LlZFHD4 transgenic plants were less tolerant to salinity and more hypersensitive to ABA compared to WT plants. The transcript levels of stress- and ABA-responsive genes were much more up-regulated in LlZFHD4 transgenic Arabidopsis than WT. These results indicate LlZFHD4 is involved in ABA signaling pathway and plays a crucial role in regulating the response of tiger lily to cold, salt and water stresses.

scholarly journals Characterization of Transcriptional Regulatory Elements in the Promoter Region of the Murine Blood Coagulation Factor VII Gene

1998 ◽  
Vol 273 (4) ◽  
pp. 2277-2287 ◽  
Author(s):  
Daniel R. Stauffer ◽  
Beatrice N. Chukwumezie ◽  
Julie A. Wilberding ◽  
Elliot D. Rosen ◽  
Francis J. Castellino
Keyword(s):  
Blood Coagulation ◽  
Promoter Region ◽  
Coagulation Factor ◽  
Regulatory Elements ◽  
Factor Vii ◽  
Transcriptional Regulatory Elements ◽  
Coagulation Factor Vii ◽  
Transcriptional Regulatory

scholarly journals Plenary Lecture 2 Transcription factors, regulatory elements and nutrient–gene communication

2009 ◽  
Vol 69 (1) ◽  
pp. 91-94 ◽  
Author(s):  
Robert J. Cousins ◽  
Tolunay B. Aydemir ◽  
Louis A. Lichten
Keyword(s):  
Transcription Factors ◽  
Histone Acetylation ◽  
Intracellular Transport ◽  
Molecular Mechanisms ◽  
Gene Activation ◽  
Regulatory Elements ◽  
Atp Citrate Lyase ◽  
Signalling Molecules ◽  
Citrate Lyase ◽  
Normal Metabolism

Dramatic advances have been made in the understanding of the differing molecular mechanisms used by nutrients to regulate genes that are essential for their biological roles to carry out normal metabolism. Classical studies have focused on nutrients as ligands to activate specific transcription factors. New interest has focused on histone acetylation as a process for either global or limited gene activation and is the first mechanism to be discussed. Nuclear ATP-citrate lyase generates acetyl-CoA, which has been shown to have a role in the activation of specific genes via selective histone acetylation. Transcription factor acetylation may provide a second mode of control of nutrient-responsive gene transcription. The third mechanism relates to the availability of response elements within chromatin, which as well as the location of the elements in the gene may allow or prevent transcription. A fourth mechanism involves intracellular transport of Zn ions, which can orchestrate localized enzyme inhibition–activation. This process in turn influences signalling molecules that regulate gene expression. The examples provided in the present review point to a new level of complexity in understanding nutrient–gene communication.

scholarly journals Molecular Characterization of a Heteromeric ATP-Citrate Lyase That Generates Cytosolic Acetyl-Coenzyme A in Arabidopsis

2002 ◽  
Vol 130 (2) ◽  
pp. 740-756 ◽  
Author(s):  
Beth L. Fatland ◽  
Jinshan Ke ◽  
Marc D. Anderson ◽  
Wieslawa I. Mentzen ◽  
Li Wei Cui ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Coenzyme A ◽  
Atp Citrate Lyase ◽  
Acetyl Coenzyme A ◽  
Acetyl Coenzyme ◽  
Citrate Lyase

scholarly journals Molecular cloning and characterization of the ATP citrate lyase from carotenogenic yeastPhaffia rhodozyma

2015 ◽  
Vol 15 (6) ◽  
pp. fov054 ◽  
Author(s):  
Cipriano Chávez-Cabrera ◽  
Rodolfo Marsch ◽  
Yaneth Bartolo-Aguilar ◽  
Zoila Rosa Flores-Bustamante ◽  
María Eugenia Hidalgo-Lara ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Atp Citrate Lyase ◽  
Citrate Lyase
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