scholarly journals Chromatin‐dependent and ‐independent regulation of DNA replication origin activation in budding yeast

EMBO Reports ◽  
2012 ◽  
Vol 14 (2) ◽  
pp. 191-198 ◽  
Author(s):  
Marko Lõoke ◽  
Kersti Kristjuhan ◽  
Signe Värv ◽  
Arnold Kristjuhan
Keyword(s):  
Dna Replication ◽  
Replication Origin ◽  
Budding Yeast ◽  
Origin Activation ◽  
Dna Replication Origin

scholarly journals Multiple Orientation-Dependent, Synergistically Interacting, Similar Domains in the Ribosomal DNA Replication Origin of the Fission Yeast, Schizosaccharomyces pombe

1998 ◽  
Vol 18 (12) ◽  
pp. 7294-7303 ◽  
Author(s):  
Soo-Mi Kim ◽  
Joel A. Huberman
Keyword(s):  
Dna Replication ◽  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Ribosomal Dna ◽  
Replication Origin ◽  
Budding Yeast ◽  
Sequence Motifs ◽  
Dna Replication Origin ◽  
Dna Replication Origins ◽  
Origin Function

ABSTRACT Previous investigations have shown that the fission yeast,Schizosaccharomyces pombe, has DNA replication origins (500 to 1500 bp) that are larger than those in the budding yeast,Saccharomyces cerevisiae (100 to 150 bp). Deletion and linker substitution analyses of two fission yeast origins revealed that they contain multiple important regions with AT-rich asymmetric (abundant A residues in one strand and T residues in the complementary strand) sequence motifs. In this work we present the characterization of a third fission yeast replication origin, ars3001, which is relatively small (∼570 bp) and responsible for replication of ribosomal DNA. Like previously studied fission yeast origins,ars3001 contains multiple important regions. The three most important of these regions resemble each other in several ways: each region is essential for origin function and is at least partially orientation dependent, each region contains similar clusters of A+T-rich asymmetric sequences, and the regions can partially substitute for each other. These observations suggest that ars3001function requires synergistic interactions between domains binding similar proteins. It is likely that this requirement extends to other fission yeast origins, explaining why such origins are larger than those of budding yeast.

scholarly journals Subcellular Proteomics Reveals a Role for Nucleo-cytoplasmic Trafficking at the DNA Replication Origin Activation Checkpoint

2013 ◽  
Vol 12 (3) ◽  
pp. 1436-1453 ◽  
Author(s):  
Claire M. Mulvey ◽  
Slavica Tudzarova ◽  
Mark Crawford ◽  
Gareth H. Williams ◽  
Kai Stoeber ◽  
...  
Keyword(s):  
Dna Replication ◽  
Replication Origin ◽  
Origin Activation ◽  
Dna Replication Origin ◽  
Subcellular Proteomics

scholarly journals Universal temporal rate of DNA replication origin firing: A balance between origin activation and passivation

2018 ◽  
Author(s):  
Jean-Michel Arbona ◽  
Arach Goldar ◽  
Olivier Hyrien ◽  
Alain Arneodo ◽  
Benjamin Audit
Keyword(s):  
Dna Replication ◽  
Replication Origin ◽  
Replication Fork ◽  
Time Dependent ◽  
Origin Firing ◽  
Dependent Rate ◽  
Origin Activation ◽  
Normal Diffusion ◽  
Dna Replication Origin ◽  
Temporal Rate

AbstractThe time-dependent rate I(t) of origin firing per length of unreplicated DNA presents a universal bell shape in eukaryotes that has been interpreted as the result of a complex time-evolving interaction between origins and limiting firing factors. Here we show that a normal diffusion of replication fork components towards localized potential replication origins (p-oris) can more simply account for the I(t) universal bell shape, as a consequence of a competition between the origin firing time and the time needed to replicate DNA separating two neighboring p-oris. We predict the I(t) maximal value to be the product of the replication fork speed with the squared p-ori density. We show that this relation is robustly observed in simulations and in experimental data for several eukaryotes. Our work underlines that fork-component recycling and potential origins localization are sufficient spatial ingredients to explain the universality of DNA replication kinetics.

scholarly journals The eukaryotic bell-shaped temporal rate of DNA replication origin firing emanates from a balance between origin activation and passivation

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Jean-Michel Arbona ◽  
Arach Goldar ◽  
Olivier Hyrien ◽  
Alain Arneodo ◽  
Benjamin Audit
Keyword(s):  
Dna Replication ◽  
Replication Origin ◽  
Replication Fork ◽  
Time Dependent ◽  
Origin Firing ◽  
Dependent Rate ◽  
Origin Activation ◽  
Normal Diffusion ◽  
Dna Replication Origin ◽  
Temporal Rate

The time-dependent rate I(t) of origin firing per length of unreplicated DNA presents a universal bell shape in eukaryotes that has been interpreted as the result of a complex time-evolving interaction between origins and limiting firing factors. Here, we show that a normal diffusion of replication fork components towards localized potential replication origins (p-oris) can more simply account for the I(t) universal bell shape, as a consequence of a competition between the origin firing time and the time needed to replicate DNA separating two neighboring p-oris. We predict the I(t) maximal value to be the product of the replication fork speed with the squared p-ori density. We show that this relation is robustly observed in simulations and in experimental data for several eukaryotes. Our work underlines that fork-component recycling and potential origins localization are sufficient spatial ingredients to explain the universality of DNA replication kinetics.

scholarly journals Quantitative Proteomics Reveals a “Poised Quiescence” Cellular State after Triggering the DNA Replication Origin Activation Checkpoint

2010 ◽  
Vol 9 (10) ◽  
pp. 5445-5460 ◽  
Author(s):  
Claire Mulvey ◽  
Slavica Tudzarova ◽  
Mark Crawford ◽  
Gareth H. Williams ◽  
Kai Stoeber ◽  
...  
Keyword(s):  
Dna Replication ◽  
Quantitative Proteomics ◽  
Replication Origin ◽  
Origin Activation ◽  
Dna Replication Origin

DNA replication origin activation in space and time

2015 ◽  
Vol 16 (6) ◽  
pp. 360-374 ◽  
Author(s):  
Michalis Fragkos ◽  
Olivier Ganier ◽  
Philippe Coulombe ◽  
Marcel Méchali
Keyword(s):  
Dna Replication ◽  
Replication Origin ◽  
Space And Time ◽  
Origin Activation ◽  
Dna Replication Origin

scholarly journals Human RIF 1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation

EMBO Reports ◽  
2017 ◽  
Vol 18 (3) ◽  
pp. 403-419 ◽  
Author(s):  
Shin‐ichiro Hiraga ◽  
Tony Ly ◽  
Javier Garzón ◽  
Zuzana Hořejší ◽  
Yoshi‐nobu Ohkubo ◽  
...  
Keyword(s):  
Dna Replication ◽  
Protein Phosphatase ◽  
Replication Origin ◽  
Protein Phosphatase 1 ◽  
Origin Activation ◽  
Origin Licensing ◽  
Dna Replication Origin
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