scholarly journals Long-term propagation of tree shrew spermatogonial stem cells in culture and successful generation of transgenic offspring

Cell Research ◽  
2016 ◽  
Vol 27 (2) ◽  
pp. 241-252 ◽  
Author(s):  
Chao-Hui Li ◽  
Lan-Zhen Yan ◽  
Wen-Zan Ban ◽  
Qiu Tu ◽  
Yong Wu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Tree Shrew ◽  
Spermatogonial Stem Cells ◽  
Cells In Culture

scholarly journals Long-term health in recipients of transplanted in vitro propagated spermatogonial stem cells

2017 ◽  
Vol 33 (1) ◽  
pp. 81-90 ◽  
Author(s):  
Callista L Mulder ◽  
Lisa A E Catsburg ◽  
Yi Zheng ◽  
Cindy M de Winter-Korver ◽  
Saskia K M van Daalen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spermatogonial Stem Cells

Long-Term Culture and Transplantation of Spermatogonial Stem Cells from BALB/c Mice

2010 ◽  
Vol 191 (5) ◽  
pp. 372-381 ◽  
Author(s):  
Fujin Shen ◽  
Ci Zhang ◽  
Hongyun Zheng ◽  
Yunhe Xiong ◽  
Xi Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spermatogonial Stem Cells ◽  
Long Term Culture

Isolating Highly Pure Rat Spermatogonial Stem Cells in Culture

2008 ◽  
pp. 163-179 ◽  
Author(s):  
F. Kent Hamra ◽  
Karen M. Chapman ◽  
Zhuoru Wu ◽  
David L. Garbers
Keyword(s):  
Stem Cells ◽  
Spermatogonial Stem Cells ◽  
Cells In Culture

scholarly journals Expansion and long-term culture of human spermatogonial stem cells via the activation of SMAD3 and AKT pathways

2015 ◽  
Vol 240 (8) ◽  
pp. 1112-1122 ◽  
Author(s):  
Ying Guo ◽  
Linhong Liu ◽  
Min Sun ◽  
Yanan Hai ◽  
Zheng Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spermatogonial Stem Cells ◽  
Long Term Culture

scholarly journals Maintenance of mouse hematopoietic stem cells ex vivo by reprogramming cellular metabolism

Blood ◽  
2015 ◽  
Vol 125 (10) ◽  
pp. 1562-1565 ◽  
Author(s):  
Xia Liu ◽  
Hong Zheng ◽  
Wen-Mei Yu ◽  
Todd M. Cooper ◽  
Kevin D. Bunting ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Ex Vivo ◽  
Cellular Metabolism ◽  
Mitochondrial Metabolism ◽  
Hematopoietic Stem ◽  
Cells In Culture ◽  
Key Points

Key Points Treatment with alexidine dihydrochloride, a Ptpmt1 inhibitor, reprograms cellular metabolism and preserves long-term stem cells ex vivo. Inhibition of mitochondrial metabolism by metformin also decreases differentiation and helps maintain stem cells in culture.

Long-term survival of human spermatogonial stem cells in mouse testes

2002 ◽  
Vol 78 ◽  
pp. S35-S36
Author(s):  
Pasquale Patrizio ◽  
Makoto Nagano ◽  
Ralph L Brinster
Keyword(s):  
Stem Cells ◽  
Spermatogonial Stem Cells ◽  
Term Survival ◽  
Long Term Survival

Genetic and epigenetic stability of human spermatogonial stem cells during long-term culture

2014 ◽  
Vol 102 (6) ◽  
pp. 1700-1707.e1 ◽  
Author(s):  
Bita Nickkholgh ◽  
S. Canan Mizrak ◽  
Saskia K.M. van Daalen ◽  
Cindy M. Korver ◽  
Hooman Sadri-Ardekani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spermatogonial Stem Cells ◽  
Long Term Culture ◽  
Genetic And Epigenetic Stability

229 LONG-TERM PROPAGATION AND CRYOPRESERVATION OF CAT SPERMATOGONIAL STEM CELLS

2016 ◽  
Vol 28 (2) ◽  
pp. 246
Author(s):  
L. M. Vansandt ◽  
M. Dickson ◽  
R. Zhou ◽  
L. Li ◽  
B. S. Pukazhenthi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Germ Cells ◽  
Spermatogonial Stem Cells ◽  
Domestic Cat ◽  
Feeder Cell ◽  
Cell Clusters ◽  
Fetal Fibroblasts

Spermatogonial stem cells (SSC) are unique adult stem cells that reside within the seminiferous tubules of the testis. As stem cells, SSC maintain the ability to self-replicate, providing a potentially unlimited supply of cells and an alternate source for preservation of the male genome. While self-renewing, long-term SSC culture has been achieved in mice, there is virtually no information regarding culture requirements of felid SSC. Therefore, the objectives of this study were to (1) evaluate the ability of 3 feeder cell lines to support germ cell colony establishment in domestic cats (Felis catus), and (2) assess long-term culture using the best feeder(s). Cells isolated enzymatically from peripubertal cat testes (n = 4) and enriched by differential plating were cultured on mouse embryonic fibroblasts (STO line), mouse-derived C166 endothelial cells, and primary cat fetal fibroblasts (cFF). Colony morphology was assessed every other day and immunocytochemistry (ICC) was performed to investigate expression of SSC markers. At 5 days in vitro (DIV), a cluster forming activity assay was used to estimate the number of SSC supported by each feeder cell line. Differences among treatments were compared using Tukey-Kramer adjustment for pair-wise mean comparisons. Data were expressed as mean cluster number ± SE per 105 cells input. When cultured on STO feeders, cat germ cells were distributed as individual cells. On both C166 cells and cFF feeders, germ cell clumps (morphologically consistent with SSC colonies in other species) were observed. Immunocytochemistry revealed that the single germ cells present on STO feeders were positive for UCHL1 and weakly expressed PLZF and OCT4. Cells within the germ cell clumps on C166 cells and cFF co-expressed all 3 SSC markers. The C166 cells supported a higher number of germ cell clusters (77.4 ± 13.8) compared with STO (3.5 ± 1.1, P = 0.0003) or cFF (22.7 ± 1.0, P = 0.0024). Therefore, subsequent subculture experiments were performed exclusively with C166 feeder layers. Cultures from 2 donors were passaged at 12 DIV and periodically as needed thereafter. Germ cell clumps consistently reestablished following each subculture and immunocytochemistry analysis confirmed maintenance of all 3 SSC markers. Cells were also positive for alkaline phosphatase activity. Cells that had been cryopreserved in culture medium with 5% (vol/vol) dimethyl sulphoxide after144 DIV (7 passages) were thawed and cultured for an additional 18 days. These cells continued to express SSC markers and form germ cell clusters. Taken together, these data demonstrate that C166 feeder cells can facilitate colony establishment and in vitro propagation of germ cell clumps in the domestic cat. This represents an important first step towards attainment and optimization of a long-term SSC culture system in the cat. This system would provide a mechanism to explore regulation of spermatogenesis, test species-specific drugs, and produce transgenic biomedical models.

Enrichment of spermatogonial stem cells from long-term cultured human testicular cells

2014 ◽  
Vol 102 (2) ◽  
pp. 558-565.e5 ◽  
Author(s):  
Bita Nickkholgh ◽  
Sefika Canan Mizrak ◽  
Cindy M. Korver ◽  
Saskia K.M. van Daalen ◽  
Andreas Meissner ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spermatogonial Stem Cells ◽  
Testicular Cells

scholarly journals Does co-transplantation of mesenchymal and spermatogonial stem cells improve reproductive efficiency and safety in mice?

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Prashant Kadam ◽  
Elissavet Ntemou ◽  
Jaime Onofre ◽  
Dorien Van Saen ◽  
Ellen Goossens
Keyword(s):  
Stem Cells ◽  
Germ Cells ◽  
Transforming Growth Factor Beta ◽  
Dna Methyltransferase ◽  
Transforming Growth Factor ◽  
Spermatogonial Stem Cells ◽  
Reproductive Efficiency ◽  
Control Group ◽  
Low Efficiency

Abstract Background Spermatogonial stem cell transplantation (SSCT) is a promising therapy in restoring the fertility of childhood cancer survivors. However, the low efficiency of SSCT is a significant concern. SSCT could be improved by co-transplanting transforming growth factor beta 1 (TGFβ1)-induced mesenchymal stem cells (MSCs). In this study, we investigated the reproductive efficiency and safety of co-transplanting spermatogonial stem cells (SSCs) and TGFβ1-induced MSCs. Methods A mouse model for long-term infertility was used to transplant SSCs (SSCT, n = 10) and a combination of SSCs and TGFβ1-treated MSCs (MSi-SSCT, n = 10). Both transplanted groups and a fertile control group (n = 7) were allowed to mate naturally to check the reproductive efficiency after transplantation. Furthermore, the testes from transplanted males and donor-derived male offspring were analyzed for the epigenetic markers DNA methyltransferase 3A (DNMT3A) and histone 4 lysine 5 acetylation (H4K5ac). Results The overall tubular fertility index (TFI) after SSCT (76 ± 12) was similar to that after MSi-SSCT (73 ± 14). However, the donor-derived TFI after MSi-SSCT (26 ± 14) was higher compared to the one after SSCT (9 ± 5; P = 0.002), even after injecting half of the number of SSCs in MSi-SSCT. The litter sizes after SSCT (3.7 ± 3.7) and MSi-SSCT (3.7 ± 3.6) were similar but differed significantly with the control group (7.6 ± 1.0; P < 0.001). The number of GFP+ offspring per litter obtained after SSCT (1.6 ± 0.5) and MSi-SSCT (2.0 ± 1.0) was also similar. The expression of DNMT3A and H4K5ac in germ cells of transplanted males was found to be significantly reduced compared to the control group. However, in donor-derived offspring, DNMT3A and H4K5ac followed the normal pattern. Conclusion Co-transplanting SSCs and TGFβ1-treated MSCs results in reproductive efficiency as good as SSCT, even after transplanting half the number of SSCs. Although transplanted males showed lower expression of DNMT3A and H4K5ac in donor-derived germ cells, the expression was restored to normal levels in germ cells of donor-derived offspring. This procedure could become an efficient method to restore fertility in a clinical setup, but more studies are needed to ensure safety in the long term.

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