scholarly journals Corneal epithelial changes following reconstruction by transplantation of limbal stem cells in goats with total limbal stem cell deficiency

Cell Research ◽  
2008 ◽  
Vol 18 (S1) ◽  
pp. S72-S72
Author(s):  
Shengli Mi ◽  
Zhongying Dou ◽  
Qingmei Zhao ◽  
Xueyi Yang ◽  
Lei Qu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Limbal Stem Cell Deficiency ◽  
Limbal Stem Cells ◽  
Corneal Epithelial ◽  
Limbal Stem Cell

scholarly journals Efficacy of Modified Amnion-Assisted Conjunctival Epithelial Redirection (ACER) for Partial Limbal Stem Cell Deficiency

Medicina ◽  
2021 ◽  
Vol 57 (4) ◽  
pp. 369
Author(s):  
Sang Beom Han ◽  
Farah Nur Ilyana Mohd Ibrahim ◽  
Yu-Chi Liu ◽  
Jodhbir S. Mehta
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Epithelial Cells ◽  
Limbal Stem Cell Deficiency ◽  
Limbal Stem Cells ◽  
Corneal Surface ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Limbal Stem Cell ◽  
Conjunctival Epithelial Cells

Background and objectives: the aim of this study was to analyze the efficacy of a modified “amnion-assisted conjunctival epithelial redirection (ACER)” technique for the treatment of partial limbal stem cell deficiency (LSCD). Materials and methods: the medical records of three patients with partial LSCD who underwent corneal surface reconstruction with modified ACER following superficial keratectomy were retrospectively studied. Briefly, in this technique, an inner amniotic membrane (AM) layer was applied on the corneal surface to promote corneal re-epithelialization. The outer AM layer was applied as a barrier to prevent the invasion of conjunctival epithelial cells into the cornea before the corneal surface was completely covered by corneal epithelial cells derived from the remaining intact limbal stem cells. Results: in all three cases, the outer AM layer successfully kept the conjunctival epithelium away from the corneal surface and prevented an admixture of conjunctival epithelial cells with corneal epithelial cells. In all three patients, the cornea was completely re-epithelized with epithelial cells derived from the remaining healthy limbal stem cells, and a clear visual axis was maintained without recurrence for a mean follow-up period of 37.3 ± 8.6 months. Conclusions: the preliminary results suggest that modified ACER appears to be a viable option for patients with partial LSCD.

scholarly journals Limbal stem cell deficiency: special focus on tracking limbal stem cells

2016 ◽  
Vol 40 ◽  
pp. 1059-1067
Author(s):  
Santhosh KACHAM ◽  
Bhaskar BIRRU ◽  
Sreenivasa Rao PARCHA ◽  
Ramaraju BAADHE
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Limbal Stem Cell Deficiency ◽  
Special Focus ◽  
Limbal Stem Cells ◽  
Limbal Stem Cell

Delayed Loss of Corneal Epithelial Stem Cells in a Chemical Injury Model Associated with Limbal Stem Cell Deficiency in Rabbits

2011 ◽  
Vol 36 (12) ◽  
pp. 1098-1107 ◽  
Author(s):  
Tamar Kadar ◽  
Vered Horwitz ◽  
Rita Sahar ◽  
Maayan Cohen ◽  
Liat Cohen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Limbal Stem Cell Deficiency ◽  
Epithelial Stem Cells ◽  
Corneal Epithelial ◽  
Chemical Injury ◽  
Injury Model ◽  
Limbal Stem Cell

Clinical Outcomes of Modified Simple Limbal Epithelial Transplantation for Limbal Stem Cell Deficiency in Chinese Population: A Retrospective Case Series

2021 ◽  
Author(s):  
Yinghui Wang ◽  
Xiaodan Hu ◽  
Ke Yang ◽  
Yang Zhang ◽  
Shijing Deng ◽  
...  
Keyword(s):  
Stem Cell ◽  
Epithelial Cells ◽  
Clinical Outcomes ◽  
Case Series ◽  
Limbal Stem Cell Deficiency ◽  
Successful Outcome ◽  
Limbal Stem Cells ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Limbal Stem Cell

Abstract Objective To report clinical outcomes of a novel surgical technique, namely simple limbal epithelial transplantation (SLET), for the treatment of limbal stem cell deficiency (LSCD). Methods 13 patients (13 eyes) with LSCD who underwent autologous (10 eyes) or allogeneic (3 eyes) modified SLET between 2018 and 2021 were enrolled in this study. Grades of symblepharon, corneal conjunctivalisation, vascularisation, opacification, and visual acuity (VA) were evaluated preoperatively and postoperatively. In 2 cases, in vivo confocal microscopy (IVCM) and impression cytology (IC) were performed to assess the proliferation and degeneration of limbal tissue. Results At a postoperative follow-up of 6.5 ± 5.3 (range, 2–20) months, 10 (10/13, 76.92%) eyes maintained a successful outcome. The grades of symblepharon, corneal conjunctivalisation, vascularisation, and opacification were significantly improved after SLET (P < 0.05). Two-line improvement in VA was found in 6 (6/10, 60%) eyes of the successful cases. Recurrence of LSCD occurred in 3 (3/13, 23.08%) eyes and conjunctival cyst occurred in 1 patient. After SLET, morphology and structure of corneal epithelial cells and epithelial transition around the limbal tissue fragments were detected by IVCM and IC. Conclusions Our findings suggest that the SLET is a safe and effective technique for the treatment of LSCD. The corneal stroma and hAM can provide protection and nutrition for the limbal stem cells (LSCs) without negatively influencing clinical outcomes. IVCM and IC after SLET can evaluate the effectiveness of surgery and the transition of LSCs and corneal epithelial cells.

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