scholarly journals Rapidly generating knockout mice from H19-Igf2 engineered androgenetic haploid embryonic stem cells

2015 ◽  
Vol 1 (1) ◽  
Author(s):  
Meili Zhang ◽  
Yufang Liu ◽  
Guang Liu ◽  
Xin Li ◽  
Yuyan Jia ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Knockout Mice ◽  
Embryonic Stem ◽  
Androgenetic Haploid

scholarly journals Generation of Genetically Modified Mice by Oocyte Injection of Androgenetic Haploid Embryonic Stem Cells

Cell ◽  
2012 ◽  
Vol 149 (3) ◽  
pp. 605-617 ◽  
Author(s):  
Hui Yang ◽  
Linyu Shi ◽  
Bang-An Wang ◽  
Dan Liang ◽  
Cuiqing Zhong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Genetically Modified ◽  
Embryonic Stem ◽  
Genetically Modified Mice ◽  
Androgenetic Haploid

Androgenetic haploid embryonic stem cells produce live transgenic mice

Nature ◽  
2012 ◽  
Vol 490 (7420) ◽  
pp. 407-411 ◽  
Author(s):  
Wei Li ◽  
Ling Shuai ◽  
Haifeng Wan ◽  
Mingzhu Dong ◽  
Meng Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Transgenic Mice ◽  
Embryonic Stem ◽  
Androgenetic Haploid

scholarly journals Derivation of Haploid Neurons from Mouse Androgenetic Haploid Embryonic Stem Cells

2017 ◽  
Vol 33 (3) ◽  
pp. 361-364 ◽  
Author(s):  
He Xu ◽  
Chunmei Yue ◽  
Ting Zhang ◽  
Yuanyuan Li ◽  
Ao Guo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Androgenetic Haploid

scholarly journals Epigenetic integrity of paternal imprints enhances the developmental potential of androgenetic haploid embryonic stem cells

2021 ◽  
Author(s):  
Hongling Zhang ◽  
Yuanyuan Li ◽  
Yongjian Ma ◽  
Chongping Lai ◽  
Qian Yu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Embryonic Stem ◽  
Early Passage ◽  
Developmental Potential ◽  
Passage Cells ◽  
Androgenetic Haploid

AbstractThe use of two inhibitors of Mek1/2 and Gsk3β (2i) promotes the generation of mouse diploid and haploid embryonic stem cells (ESCs) from the inner cell mass of biparental and uniparental blastocysts, respectively. However, a system enabling long-term maintenance of imprints in ESCs has proven challenging. Here, we report that the use of a two-step a2i (alternative two inhibitors of Src and Gsk3β, TSa2i) derivation/culture protocol results in the establishment of androgenetic haploid ESCs (AG-haESCs) with stable DNA methylation at paternal DMRs (differentially DNA methylated regions) up to passage 60 that can efficiently support generating mice upon oocyte injection. We also show coexistence of H3K9me3 marks and ZFP57 bindings with intact DMR methylations. Furthermore, we demonstrate that TSa2i-treated AG-haESCs are a heterogeneous cell population regarding paternal DMR methylation. Strikingly, AG-haESCs with late passages display increased paternal-DMR methylations and improved developmental potential compared to early-passage cells, in part through the enhanced proliferation of H19-DMR hypermethylated cells. Together, we establish AG-haESCs that can long-term maintain paternal imprints.

scholarly journals Reduced Self-Diploidization and Improved Survival of Semi-cloned Mice Produced from Androgenetic Haploid Embryonic Stem Cells through Overexpression of Dnmt3b

2018 ◽  
Vol 10 (2) ◽  
pp. 477-493 ◽  
Author(s):  
Wenteng He ◽  
Xiaobai Zhang ◽  
Yalin Zhang ◽  
Weisheng Zheng ◽  
Zeyu Xiong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Androgenetic Haploid
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