Androgenetic haploid embryonic stem cells produce live transgenic mice

Nature ◽  
2012 ◽  
Vol 490 (7420) ◽  
pp. 407-411 ◽  
Author(s):  
Wei Li ◽  
Ling Shuai ◽  
Haifeng Wan ◽  
Mingzhu Dong ◽  
Meng Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Transgenic Mice ◽  
Embryonic Stem ◽  
Androgenetic Haploid

The Use of Genetically Modified Embryonic Stem Cells to Generate Transgenic Mice

2004 ◽  
Vol 40 (3) ◽  
pp. 235-238 ◽  
Author(s):  
M. V. Pryzhkova ◽  
I. R. Zakeeva ◽  
A. V. Kibardin ◽  
G. P. Georgiev ◽  
S. L. Kiselev
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Transgenic Mice ◽  
Genetically Modified ◽  
Embryonic Stem

scholarly journals Generation of Genetically Modified Mice by Oocyte Injection of Androgenetic Haploid Embryonic Stem Cells

Cell ◽  
2012 ◽  
Vol 149 (3) ◽  
pp. 605-617 ◽  
Author(s):  
Hui Yang ◽  
Linyu Shi ◽  
Bang-An Wang ◽  
Dan Liang ◽  
Cuiqing Zhong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Genetically Modified ◽  
Embryonic Stem ◽  
Genetically Modified Mice ◽  
Androgenetic Haploid

scholarly journals  -Synuclein Alters Notch-1 Expression and Neurogenesis in Mouse Embryonic Stem Cells and in the Hippocampus of Transgenic Mice

2008 ◽  
Vol 28 (16) ◽  
pp. 4250-4260 ◽  
Author(s):  
L. Crews ◽  
H. Mizuno ◽  
P. Desplats ◽  
E. Rockenstein ◽  
A. Adame ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Transgenic Mice ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Notch 1

scholarly journals Derivation of Haploid Neurons from Mouse Androgenetic Haploid Embryonic Stem Cells

2017 ◽  
Vol 33 (3) ◽  
pp. 361-364 ◽  
Author(s):  
He Xu ◽  
Chunmei Yue ◽  
Ting Zhang ◽  
Yuanyuan Li ◽  
Ao Guo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Androgenetic Haploid

scholarly journals Epigenetic integrity of paternal imprints enhances the developmental potential of androgenetic haploid embryonic stem cells

2021 ◽  
Author(s):  
Hongling Zhang ◽  
Yuanyuan Li ◽  
Yongjian Ma ◽  
Chongping Lai ◽  
Qian Yu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Embryonic Stem ◽  
Early Passage ◽  
Developmental Potential ◽  
Passage Cells ◽  
Androgenetic Haploid

AbstractThe use of two inhibitors of Mek1/2 and Gsk3β (2i) promotes the generation of mouse diploid and haploid embryonic stem cells (ESCs) from the inner cell mass of biparental and uniparental blastocysts, respectively. However, a system enabling long-term maintenance of imprints in ESCs has proven challenging. Here, we report that the use of a two-step a2i (alternative two inhibitors of Src and Gsk3β, TSa2i) derivation/culture protocol results in the establishment of androgenetic haploid ESCs (AG-haESCs) with stable DNA methylation at paternal DMRs (differentially DNA methylated regions) up to passage 60 that can efficiently support generating mice upon oocyte injection. We also show coexistence of H3K9me3 marks and ZFP57 bindings with intact DMR methylations. Furthermore, we demonstrate that TSa2i-treated AG-haESCs are a heterogeneous cell population regarding paternal DMR methylation. Strikingly, AG-haESCs with late passages display increased paternal-DMR methylations and improved developmental potential compared to early-passage cells, in part through the enhanced proliferation of H19-DMR hypermethylated cells. Together, we establish AG-haESCs that can long-term maintain paternal imprints.

Rapid selection of XO embryonic stem cells using Y chromosome-linked GFP transgenic mice

2014 ◽  
Vol 23 (5) ◽  
pp. 757-765 ◽  
Author(s):  
Satoshi Yamamoto ◽  
Yasumitsu Nagao ◽  
Kenji Kuroiwa ◽  
Yoji Hakamata ◽  
Masaru Ichida ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Transgenic Mice ◽  
Y Chromosome ◽  
Embryonic Stem ◽  
Selection Of
Sign in / Sign up

Export Citation Format

Share Document