scholarly journals Skin-derived stem cells as a source of primordial germ cell- and oocyte-like cells

2016 ◽  
Vol 7 (11) ◽  
pp. e2471-e2471 ◽  
Author(s):  
Wei Ge ◽  
Shun-Feng Cheng ◽  
Paul W Dyce ◽  
Massimo De Felici ◽  
Wei Shen
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Primordial Germ Cell

scholarly journals Primordial Germ Cell-Like Cells Differentiated In Vitro from Skin-Derived Stem Cells

PLoS ONE ◽  
2009 ◽  
Vol 4 (12) ◽  
pp. e8263 ◽  
Author(s):  
Katja Linher ◽  
Paul Dyce ◽  
Julang Li
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Primordial Germ Cell

Primordial germ cell differentiation of nuclear transfer embryonic stem cells using surface modified electroconductive scaffolds

2016 ◽  
Vol 53 (4) ◽  
pp. 371-380
Author(s):  
Tarlan Eslami-Arshaghi ◽  
Saeid Vakilian ◽  
Ehsan Seyedjafari ◽  
Abdolreza Ardeshirylajimi ◽  
Masoud Soleimani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Differentiation ◽  
Embryonic Stem Cells ◽  
Germ Cell ◽  
Nuclear Transfer ◽  
Primordial Germ Cell ◽  
Embryonic Stem ◽  
Germ Cell Differentiation ◽  
Surface Modified

154 MOLECULAR CHARACTERIZATION OF CAT PRIMORDIAL GERM CELLS

2016 ◽  
Vol 28 (2) ◽  
pp. 207
Author(s):  
J. Galiguis ◽  
C. E. Pope ◽  
C. Dumas ◽  
G. Wang ◽  
R. A. MacLean ◽  
...  
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Primordial Germ Cell ◽  
Embryonic Stem ◽  
Transcript Abundance ◽  
Domestic Cat ◽  
Sample Type

As precursors to germline stem cells and gametes, there are many potential applications for primordial germ cells (PGC). Primordial germ cell-like cells have been generated from mouse embryonic stem cells and induced pluripotent stem cells, which subsequently were used to produce functional spermatozoa, oocytes, and healthy offspring (Hayashi et al. 2012 Science 338(6109), 971–975). Applying this approach to generate sperm and oocytes of endangered species is an appealing prospect. Detection of molecular markers associated with PGC is essential to optimizing the process of PGC induction. In the current study, in vitro-derived domestic cat embryos were assessed at various developmental stages to characterise the expression of markers related to the specification process of cat PGC. In vivo-matured, IVF oocytes were cultured until Days 7, 9, and 12 post-insemination. Then, embryos were assessed by RT-qPCR to determine relative transcript abundance of the pluripotency markers NANOG, POU5F1, and SOX2; the epiblast marker DNMT3B; the primitive endoderm marker GATA4; the PGC marker PRDM14; and the germ cell marker VASA; RPS19 was used as the internal reference gene. To validate the qPCR results, fibroblasts served as the negative control cells, whereas spermatogonial stem cells (SSC) served as the positive control cells for GATA4, PRDM14, and VASA. Total mRNA were isolated using the Cells-to-cDNA™ II Kit (Ambion/Thermo Fisher Scientific, Waltham, MA, USA) from either pools of 2 to 6 embryos or ~25 000 fibroblasts/SSC. A minimum of 2 biological replicates for each sample type was analysed, with transcript abundance detected in 2 technical replicates by SYBR Green chemistry. Student’s t-tests were performed on the ΔCts for statistical analysis. PRDM14, specific to the germ cell lineage, was detected as early as Day 7, suggesting the presence of PGC precursor cells. Compared with their levels at Day 7, PRDM14 expression was 0.34-fold lower in SSC (P < 0.05), whereas expression of VASA and GATA4 were 1964-fold and 144-fold higher, respectively (P < 0.05). This seems to emphasise the relative importance of PRDM14 in pre-germ cell stages. In general, all genes analysed were up-regulated from Day 7 to Day 9. This up-regulation was statistically significant for SOX2 and GATA4 (P < 0.05). Relative to that at Day 9, all transcripts were relatively less abundant at Day 12 (P < 0.05 for NANOG, POU5F1, SOX2, DNMT3B, and PRDM14). The data suggest that PGC specification takes place near Day 9, with peak specification activity concluding by Day 12. Although much needs be explored about PGC specification in the cat before applying induction and in vitro germ cell production techniques, these findings represent the first step towards a new potential strategy for preserving endangered and threatened felids.

scholarly journals Primordial germ cell-like cells derived from canine adipose mesenchymal stem cells

2016 ◽  
Vol 49 (4) ◽  
pp. 503-511 ◽  
Author(s):  
Yudong Wei ◽  
Jia Fang ◽  
Shufang Cai ◽  
Changrong Lv ◽  
Shiqiang Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Germ Cell ◽  
Primordial Germ Cell ◽  
Adipose Mesenchymal Stem Cells

scholarly journals The crucial role of Activin A on the formation of primordial germ cell-like cells from skin-derived stem cells in vitro

Cell Cycle ◽  
2015 ◽  
Vol 14 (19) ◽  
pp. 3016-3029 ◽  
Author(s):  
Rui Sun ◽  
Yuan-Chao Sun ◽  
Wei Ge ◽  
Hui Tan ◽  
Shun-Feng Cheng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Crucial Role ◽  
Primordial Germ Cell ◽  
Activin A

scholarly journals Resf1 supports embryonic stem cell self-renewal and effective germline entry

2021 ◽  
Author(s):  
Matus Vojtek ◽  
Ian Chambers
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Germ Line ◽  
Primordial Germ Cell ◽  
Embryonic Stem ◽  
Cell Specification ◽  
Self Renewal ◽  
Downstream Target ◽  
Germ Cell Specification

Retroelement silencing factor 1 (Resf1) interacts with the key regulators of mouse embryonic stem cells (ESCs) Oct4 and Nanog, and its absence results in sterility of mice. However, the function of Resf1 in ESCs and germ line specification is poorly understood. In this study, we used Resf1 knockout cell lines to determine the requirements of RESF1 for ESCs self-renewal and for in vitro specification of ESCs into primordial germ cell-like cells (PGCLCs). We found that deletion of Resf1 in ESCs cultured in serum and LIF reduces self-renewal potential whereas episomal expression of RESF1 has a modest positive effect on ESC self-renewal. In addition, RESF1 is not required for the capacity of NANOG and its downstream target ESRRB to drive self-renewal in the absence of LIF. However, Resf1 deletion reduces efficiency of PGCLC differentiation in vitro. These results identify Resf1 as a novel player in the regulation of pluripotent stem cells and germ cell specification.

scholarly journals Induction of Primordial Germ Cell-Like Cells From Mouse Embryonic Stem Cells by ERK Signal Inhibition

Stem Cells ◽  
2014 ◽  
Vol 32 (10) ◽  
pp. 2668-2678 ◽  
Author(s):  
Tohru Kimura ◽  
Yoshiaki Kaga ◽  
Hiroshi Ohta ◽  
Mika Odamoto ◽  
Yoichi Sekita ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Cell ◽  
Primordial Germ Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells
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