Evidence for a novel parathyroid-specific signaling pathway in calcium homeostasis

IBMS BoneKEy ◽  
2014 ◽  
Vol 11 ◽  
Keyword(s):  
Signaling Pathway ◽  
Calcium Homeostasis ◽  
Specific Signaling Pathway

scholarly journals Signaling-mediated cooperativity between glycoprotein Ib-IX and protease-activated receptors in thrombin-induced platelet activation

Blood ◽  
2016 ◽  
Vol 127 (5) ◽  
pp. 626-636 ◽  
Author(s):  
Brian Estevez ◽  
Kyungho Kim ◽  
M. Keegan Delaney ◽  
Aleksandra Stojanovic-Terpo ◽  
Bo Shen ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Platelet Activation ◽  
Cytoplasmic Domain ◽  
Platelet Response ◽  
Protease Activated Receptors ◽  
Glycoprotein Ib ◽  
Low Concentrations ◽  
Key Points ◽  
Specific Signaling Pathway

Key Points GPIb-IX signaling cooperates with PAR signaling to promote platelet response to low concentrations of thrombin, which are important in vivo. Thrombin induces a GPIb-IX–specific signaling pathway that requires the cytoplasmic domain of GPIbα, 14-3-3 protein, Rac1, and LIMK1.

scholarly journals SignaFish: a zebrafish-specific signaling pathway resource

2016 ◽  
Author(s):  
Kitti Csályi ◽  
Dávid Fazekas ◽  
Tamás Kadlecsik ◽  
Dénes Türei ◽  
Leila Gul ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Fruit Fly ◽  
Model Organisms ◽  
Signaling Networks ◽  
Cell Fates ◽  
Invertebrate Model ◽  
Signaling Interactions ◽  
Model Fish Species ◽  
Specific Signaling Pathway ◽  
Model Fish

Understanding living systems requires an in depth knowledge of the signaling networks that drive cellular homeostasis, regulate intercellular communication and contribute to cell fates during development. Several resources exist to provide high-throughput datasets or manually curated interaction information from human or invertebrate model organisms. We previously developed SignaLink, a uniformly curated, multi-layered signaling resource containing information for human and for the model organisms nematode Caenorhabditis elegans and fruit fly Drosophila melanogaster. Until now, the use of the SignaLink database for zebrafish pathway analysis was limited. To overcome this limitation we created SignaFish (http://signafish.org), a fish-specific signaling resource, built using the concept of SignaLink. SignaFish contains more than 200 curation based signaling interactions, 132 further interactions listed in other resources, and it also lists potential miRNA based regulatory connections for 7 major signaling pathways. From the SignaFish website, users can reach other web resources, such as ZFIN. SignaFish provides signaling or signaling-related interactions that can be examined for each gene, or downloaded for each signaling pathway. We believe that the SignaFish resource will serve as a novel navigating point for experimental design and evaluation for the zebrafish community and for researchers focusing on non-model fish species, such as cyclids.

Abstract MP239: Hippo Signaling Pathway Maintains Homeostasis Of The Cardiac Conduction System

2021 ◽  
Vol 129 (Suppl_1) ◽  
Author(s):  
Mingjie Zheng ◽  
Jun Wang
Keyword(s):  
Signaling Pathway ◽  
Calcium Homeostasis ◽  
Cardiac Arrhythmias ◽  
Critical Role ◽  
Conduction System ◽  
Conditional Knockout ◽  
Cardiac Conduction ◽  
Cardiac Conduction System ◽  
Hippo Signaling ◽  
Hippo Signaling Pathway

The cardiac conduction system (CCS) is required for initiating and maintaining regular rhythmic heartbeats. The fundamental Hippo signaling pathway plays critical roles in the heart, yet its role in the CCS remains largely unknown. Here, we found that conditional knockout (CKO) of Hippo signaling kinases Lats1 and Lats2 in the CCS using Hcn4 CreERT2 , led to cardiac arrhythmias in adult mice. Compared with controls, Lats1/2 CKO mutant mice had disrupted calcium homeostasis, increased fibrosis and more fibroblast proliferation in the sinoatrial node. Deletion of the Hippo signaling effectors Yap and Taz in the CCS rescued phenotypes caused by Lats1/2 deletion, and these mice had rescued sinus rhythm and reduced fibrosis, which indicated that Lats1/2 function through Yap and Taz in CCS. Our Cleavage Under Targets and Tagmentation (CUT&Tag)-sequencing using Yap antibody followed by RNA-Seq revealed that Yap directly regulates calcium homeostasis genes such as Ryr2 and fibrosis induction genes such as TGF-β family. Further, we discovered that miR-17-92 represses Hippo signaling by directly suppressing Lats2 expression. miR-17-92 CKO in the CCS led to increased Hippo signaling activity and cardiac arrhythmias, indicating that a fine-tuned level of Hippo signaling is critical for CCS homeostasis. Together, our findings reveal the critical role of a miR-Hippo-Yap genetic pathway in maintaining CCS homeostasis.

scholarly journals Growth Factor-specific Signaling Pathway Stimulation and Gene Expression Mediated by ErbB Receptors

2001 ◽  
Vol 276 (25) ◽  
pp. 22685-22698 ◽  
Author(s):  
Colleen Sweeney ◽  
Douglas Fambrough ◽  
Christine Huard ◽  
A. John Diamonti ◽  
Eric S. Lander ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Factor ◽  
Signaling Pathway ◽  
Erbb Receptors ◽  
Specific Signaling Pathway

scholarly journals Mesangial Cells and Glomerular Endothelial Cells Crosstalk in Rat Anti-Thy-1 Nephritis through VEGFA/VEGFAR2 and Angpt2/Tie2 pathway

2020 ◽  
Author(s):  
yinghua zhao ◽  
Bo Fu ◽  
Pu Chen ◽  
Qinggang Li ◽  
Qing Ouyang ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Signaling Pathway ◽  
Mesangial Cells ◽  
Proliferative Glomerulonephritis ◽  
Mesangial Proliferative Glomerulonephritis ◽  
Surface Receptor ◽  
Mimic Peptide ◽  
Specific Signaling Pathway

Abstract BACKGROUNDMesangial proliferative glomerulonephritis is characterized by the proliferation of mesangial cells (MCs). Endothelial cells (ECs) are affected by signals from MCs, resulting in capillary proliferation, but the specific signaling pathway associated with this activity remains unclear.RESULTSIn this study, the expression of PCNA, RECA-1 and CD34 in the glomeruli increased on the 7th day after anti-Thy-1 nephritis establishment, indicating the occurrence of ECs proliferation. After coculturing MCs and ECs in vitro, we observed that activated MCs could promote ECs proliferation, migration and α-SMA expression. Moreover, activated ECs had the same effects on MCs. RT-qPCR showed that activated MCs could increasingly secrete VEGFA, and Angpt2 expression in VEGFA-activated ECs was enhanced. Considering that Angpt2-mediated inhibition of ECs surface receptor Tie2 phosphorylation causes ECs proliferation, we hypothesized that VEGFA/VEGFR2 and Angpt2/Tie2 signaling is involved in the interaction between MCs and ECs. Our results showed that blocking VEGFA or adding the Angpt2 antagonist Angpt1 to the coculture system decreased the number of EdU-positive cells，Angpt2，p-VEGFR2 and p-MAPK expression, but increased p-Tie2 in ECs. To determine whether Angpt1 could effectively alleviate the pathological changes of anti-Thy-1 nephritis, we performed Vasculotide (Angpt1 mimic peptide) treatment assays in vivo. The results confirmed that the addition of Vasculotide could effectively reduce PCNA, RECA-1 and α-SMA expression and promote p-Tie2.CONCLUSIONIn summary, the study showed that the VEGFA/VEGFR2 and Angpt2/Tie2 signaling pathway mediate interactions between MCs and ECs, providing an important theoretical basis for the treatment of mesangial proliferative glomerulonephritis.

scholarly journals SignaFish: A Zebrafish-Specific Signaling Pathway Resource

Zebrafish ◽  
2016 ◽  
Vol 13 (6) ◽  
pp. 541-544 ◽  
Author(s):  
Kitti Csályi ◽  
Dávid Fazekas ◽  
Tamás Kadlecsik ◽  
Dénes Türei ◽  
Leila Gul ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Specific Signaling Pathway

scholarly journals RhoA triggers a specific signaling pathway that generates transforming microvesicles in cancer cells

Oncogene ◽  
2012 ◽  
Vol 31 (45) ◽  
pp. 4740-4749 ◽  
Author(s):  
B Li ◽  
M A Antonyak ◽  
J Zhang ◽  
R A Cerione
Keyword(s):  
Cancer Cells ◽  
Signaling Pathway ◽  
Specific Signaling Pathway
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