scholarly journals Role of calcium in substance P-induced chemokine synthesis in mouse pancreatic acinar cells

2008 ◽  
Vol 154 (6) ◽  
pp. 1339-1348 ◽  
Author(s):  
R D Ramnath ◽  
J Sun ◽  
M Bhatia
Keyword(s):  
Substance P ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells

Phosphoinositide synthesis in desensitized rat pancreatic acinar cells

1995 ◽  
Vol 268 (6) ◽  
pp. G1043-G1050
Author(s):  
J. S. Lods ◽  
B. Rossignol ◽  
C. Dreux ◽  
J. Morisset
Keyword(s):  
Phorbol Ester ◽  
Inositol Trisphosphate ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells ◽  
Basal Rate ◽  
Phosphoinositide Turnover ◽  
Dose Dependent

To help understand the possible role of phosphoinositide turnover in the desensitization process, the availability of phosphatidylinositol 4,5-bisphosphate was investigated in normal and desensitized pancreatic acinar cells treated with carbamylcholine (Cch), caerulein (Cae), and the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). In control acini, incorporation of [myo-3H]inositol into total phosphoinositides was maximal at 120 min, was Cch and Cae dose dependent, and was insensitive to TPA. Cch stimulation increased the proportion of [myo-3H]inositol incorporated into phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2], whereas Cae specifically channeled [myo-3H]inositol incorporation into phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate. In the desensitized cells, preexposure to Cch and Cae, but not to TPA, increased the subsequent basal rate of [myo-3H]inositol incorporation into total phosphoinositol (PI) by 66 and 50% above control values. There were no subsequent responses to increasing concentrations of Cch, Cae, and TPA during a second incubation. Desensitization of the pancreatic secretory responses to Cch, Cae, and TPA does not seem to result from a decrease either in total PI or in specific PtdIns(4,5)P2 synthesis, which is needed for inositol trisphosphate and diacylglycerol production.

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