Role of Mitogen-Activated Protein Kinases, NF- B, and AP-1 on Cerulein-Induced IL-8 Expression in Pancreatic Acinar Cells

2006 ◽  
Vol 1090 (1) ◽  
pp. 368-374 ◽  
Author(s):  
K. D. JU ◽  
J. H. YU ◽  
H. KIM ◽  
K. H. KIM
Keyword(s):  
Protein Kinases ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells ◽  
Mitogen Activated Protein Kinases ◽  
Mitogen Activated Protein

scholarly journals Role of mitogen-activated protein kinases in pulmonary endothelial cells exposed to cyclic strain

2000 ◽  
Vol 89 (6) ◽  
pp. 2391-2400 ◽  
Author(s):  
Hiroyuki Kito ◽  
Emery L. Chen ◽  
Xiujie Wang ◽  
Masataka Ikeda ◽  
Nobuyoshi Azuma ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Protein Kinases ◽  
Cyclic Strain ◽  
Cell Alignment ◽  
Mitogen Activated Protein Kinases ◽  
Mitogen Activated Protein ◽  
Pulmonary Arterial ◽  
And Migration ◽  
Sb 203580

The aim of this study was to examine the role of mitogen-activated protein kinases (MAPKs) activation in bovine pulmonary arterial endothelial cells (EC) exposed to cyclic strain. EC were subjected to 10% average strain at 60 cycles/min. Cyclic strain induced activation of extracellular signal-regulated kinase (ERK; 1.5-fold), c-Jun NH2-terminal protein kinase (JNK; 1.9-fold), and p38 (1.5-fold) with a peak at 30 min. To investigate the functional role of the activated MAPKs, we analyzed cells after treatment with PD-98059, a specific ERK kinase inhibitor, or SB-203580, a catalytic inhibitor for p38, and after transient transfection with JNK(K-R), and MEKK(K-M) the respective catalytically inactive mutants of JNK1 and MAPK kinase kinase-1. Cyclic strain increased activator protein-1 (AP-1) binding activity, which was blocked by PD-98059 and SB-203580. Activity of AP-1-dependent luciferase reporter driven by 12- O-tetradecanoyl-phorbol-13-acetate-responsive element (TRE) was induced by cyclic strain, and this was attenuated by PD-98059, MEKK(K-M), JNK(K-R), and SB-203580. PD-98059 and SB-203850 did not inhibit cell alignment and migration induced by cyclic strain. MEKK(K-M) and JNK(K-R) transfection did not block cyclic strain-induced cell alignment. In conclusion, cyclic strain activates ERK, JNK, and p38, and their activation plays a role in transcriptional activation of AP-1/TRE but not in cell alignment and migration changes in bovine pulmonary arterial EC.

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