scholarly journals Sensitivity to RNase treatment of ribosomes and rRNA from normal rat liver and Novikoff hepatoma

1982 ◽  
Vol 45 (1) ◽  
pp. 140-143 ◽  
Author(s):  
C Peclet ◽  
G de Lamirande ◽  
R Daoust
Keyword(s):  
Rat Liver ◽  
Novikoff Hepatoma ◽  
Normal Rat ◽  
Rnase Treatment

Role of cell surface receptor mobility in concanavalin A-induced agglutination of Novikoff hepatoma and normal rat liver cells

1980 ◽  
Vol 42 (1) ◽  
pp. 169-182
Author(s):  
P. Mastromarino ◽  
G. Neri ◽  
A. Serra ◽  
E.F. Walborg
Keyword(s):  
Cell Surface ◽  
Rat Liver ◽  
Liver Cells ◽  
Cell Surface Receptor ◽  
Lateral Mobility ◽  
Con A ◽  
Novikoff Hepatoma ◽  
Surface Fluorescence ◽  
Rat Liver Cells ◽  
Normal Rat

The relationshio between Con A-receptor mobility and Con A-induced agglutination of Novikoff hepatoma and normal rat liver cells was investigated. Novikoff cells, incubated with fluorescein-labelled Con A at 3 degrees C displayed uniform, ring-like surface fluorescence. Increasing the temperature of the cells to 37 degrees C caused capping of Con A receptors in approximately 65% of the cells, a phenomenon that could be prevented by prefixing the cells with glutaraldehyde. In spite of these variations in Con A-receptor distribution, Con A-induced agglutination was remarkably constant over a temperature range from 3 to 37 degrees C. In contrast to Novikoff cells, normal hepatocytes displayed a uniform, ringlike surface fluorescence at both 3 and 37 degrees C. No capping was observed. However, hepatocytes, similar to Novikoff cells, were agglutinable by low concentrations of Con A. These findings indicate that, in this model system, Con A-induced cytoagglutination is not dependent upon long-range lateral mobility of Con A receptors. The qualitative differences in the lateral mobility of cell-surface Con A receptors of normal and malignant rat liver cells may represent a marker for neoplastioc transformation during hepatocarcinogenesis, adaptation to growth in ascitic form, or progression of a tumour to a more malignant state.

scholarly journals Studies on lipid peroxidation in normal and tumour tissues. The Yoshida rat liver tumour

1988 ◽  
Vol 250 (1) ◽  
pp. 247-252 ◽  
Author(s):  
K H Cheeseman ◽  
S Emery ◽  
S P Maddix ◽  
T F Slater ◽  
G W Burton ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Rat Liver ◽  
Alpha Tocopherol ◽  
Novikoff Hepatoma ◽  
Nadph Cytochrome ◽  
Transport Chain ◽  
Initiation And Propagation ◽  
Normal Rat ◽  
Cytochrome P 450 ◽  
Nadph Cytochrome C Reductase

Reduced rates of lipid peroxidation have been observed in Yoshida hepatoma cells and microsomes when compared with appropriate control tissue (normal rat liver) under the same pro-oxidant conditions. The pro-oxidant conditions used were incubation with NADPH+ADP+iron or ascorbate+iron or exposure to gamma-irradiation. As previously shown with the Novikoff hepatoma, the relative concentrations of alpha-tocopherol and polyunsaturated fatty acids are important in conferring resistance to lipid peroxidation in the Yoshida hepatoma. Furthermore, NADPH-cytochrome c reductase and the NADPH-cytochrome P-450 electron transport chain, which are involved in the initiation and propagation of certain types of lipid peroxidation, are found at very much reduced levels in the Yoshida hepatoma. The relative importance of these aberrations are discussed.

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