scholarly journals Isolation and characterization of epithelial stem-cell cell lines from the rat mammary gland

1980 ◽  
Vol 41 (4) ◽  
pp. 666-668 ◽  
Author(s):  
P S Rudland ◽  
D C Bennett ◽  
M J Warburton
Keyword(s):  
Stem Cell ◽  
Mammary Gland ◽  
Cell Lines ◽  
Epithelial Stem Cell ◽  
Isolation And Characterization ◽  
Rat Mammary Gland ◽  
Cell Cell

The Isolation and Characterization of Fatty-Acid-Synthetase mRNA from Rat Mammary Gland

1981 ◽  
Vol 114 (3) ◽  
pp. 643-651 ◽  
Author(s):  
John S. MATTICK ◽  
Zendra E. ZEHNER ◽  
Michael A. CALABRO ◽  
Salih J. WAKIL
Keyword(s):  
Mammary Gland ◽  
Fatty Acid ◽  
Fatty Acid Synthetase ◽  
Isolation And Characterization ◽  
Rat Mammary Gland

S5.12 Isolation and characterization of proteoglycans from haematopoetic stem cell- and stromal cell-lines

1993 ◽  
Vol 10 (4) ◽  
pp. 253-254
Author(s):  
Z. Drzeniek ◽  
G. St�cker ◽  
U. Just ◽  
W. Ostertag ◽  
H. -D. Haubeck ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Stromal Cell ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Novel Rhesus Monkey Embryonic Stem Cell Lines

Stem Cells ◽  
2006 ◽  
Vol 24 (10) ◽  
pp. 2177-2186 ◽  
Author(s):  
Shoukhrat Mitalipov ◽  
Hung-Chih Kuo ◽  
James Byrne ◽  
Lisa Clepper ◽  
Lorraine Meisner ◽  
...  
Keyword(s):  
Stem Cell ◽  
Rhesus Monkey ◽  
Embryonic Stem Cell ◽  
Cell Lines ◽  
Embryonic Stem ◽  
Isolation And Characterization ◽  
Stem Cell Lines

scholarly journals Isolation and Characterization of an Adult Stem Cell Population from Human Epidural Fat

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Nedaa Al-Jezani ◽  
Roger Cho ◽  
Anand O. Masson ◽  
Brian Lenehan ◽  
Roman Krawetz ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Cell Surface Receptor ◽  
Stem Cell Population ◽  
Collagen Gels ◽  
Isolation And Characterization ◽  
Collagen Contraction ◽  
Epidural Fat

Study Design. Isolation and characterization of human epidural fat (HEF) stem/progenitor cells. Objective. To identify a progenitor population within HEF and to determine if they meet the minimal criteria of a mesenchymal stem cell (MSC). Summary of Background Data. The biological function, if any, has yet to be determined for HEF. The presence of MSCs within HEF may indicate a regenerative potential within the HEF. Methods. HEF was isolated from 10 patients during elective spinal surgery. HEF cells were differentiated along osteo-, adipo-, and chondrogenic lineages, with differentiation analyzed via qPCR and histology. The cell surface receptor profile of HEF cells was examined by flow cytometry. HEF cells were also assayed through the collagen contraction assay. Prx1CreERT2GFP:R26RTdTomato MSC lineage-tracking mice were employed to identify EF MSCs in vivo. Results. HEF cell lines were obtained from all 10 patients in the study. Cells from 2/10 patients demonstrated full MSC potential, while cells from 6/10 patients demonstrated progenitor potential; 2/10 patients presented with cells that retained only adipogenic potential. HEF cells demonstrated MSC surface marker expression. All patient cell lines contracted collagen gels. A Prx1-positive population in mouse epidural fat that appeared to contribute to the dura of the spinal cord was observed in vivo. Conclusions. MSC and progenitor populations are present within HEF. MSCs were not identified in all patients examined in the current study. Furthermore, all patient lines demonstrated collagen contraction capacity, suggesting either a contaminating activated fibroblast population or HEF MSCs/progenitors also demonstrating a fibroblast-like phenotype. In vivo analysis suggests that these cell populations may contribute to the dura. Overall, these results suggest that cells within epidural fat may play a biological role within the local environment above providing a mechanical buffer.

Isolation and characterization of human gastric cell lines with stem cell phenotypes

2007 ◽  
Vol 22 (9) ◽  
pp. 1460-1468 ◽  
Author(s):  
Yuan-Chieh Yang ◽  
Sheng-Wen Wang ◽  
Hsiao-Yen Hung ◽  
Chia-Cheng Chang ◽  
I-Chen Wu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Gastric Cell ◽  
Isolation And Characterization ◽  
Cell Phenotypes
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