S5.12 Isolation and characterization of proteoglycans from haematopoetic stem cell- and stromal cell-lines

1993 ◽  
Vol 10 (4) ◽  
pp. 253-254
Author(s):  
Z. Drzeniek ◽  
G. St�cker ◽  
U. Just ◽  
W. Ostertag ◽  
H. -D. Haubeck ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Stromal Cell ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Novel Rhesus Monkey Embryonic Stem Cell Lines

Stem Cells ◽  
2006 ◽  
Vol 24 (10) ◽  
pp. 2177-2186 ◽  
Author(s):  
Shoukhrat Mitalipov ◽  
Hung-Chih Kuo ◽  
James Byrne ◽  
Lisa Clepper ◽  
Lorraine Meisner ◽  
...  
Keyword(s):  
Stem Cell ◽  
Rhesus Monkey ◽  
Embryonic Stem Cell ◽  
Cell Lines ◽  
Embryonic Stem ◽  
Isolation And Characterization ◽  
Stem Cell Lines

scholarly journals Isolation and characterization of patient-derived CNS metastasis-associated stromal cell lines

Oncogene ◽  
2019 ◽  
Vol 38 (21) ◽  
pp. 4002-4014 ◽  
Author(s):  
Ben Yi Tew ◽  
Christophe Legendre ◽  
Gerald C. Gooden ◽  
Kyle N. Johnson ◽  
Rae Anne Martinez ◽  
...  
Keyword(s):  
Cell Lines ◽  
Stromal Cell ◽  
Cns Metastasis ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of an Adult Stem Cell Population from Human Epidural Fat

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Nedaa Al-Jezani ◽  
Roger Cho ◽  
Anand O. Masson ◽  
Brian Lenehan ◽  
Roman Krawetz ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Cell Surface Receptor ◽  
Stem Cell Population ◽  
Collagen Gels ◽  
Isolation And Characterization ◽  
Collagen Contraction ◽  
Epidural Fat

Study Design. Isolation and characterization of human epidural fat (HEF) stem/progenitor cells. Objective. To identify a progenitor population within HEF and to determine if they meet the minimal criteria of a mesenchymal stem cell (MSC). Summary of Background Data. The biological function, if any, has yet to be determined for HEF. The presence of MSCs within HEF may indicate a regenerative potential within the HEF. Methods. HEF was isolated from 10 patients during elective spinal surgery. HEF cells were differentiated along osteo-, adipo-, and chondrogenic lineages, with differentiation analyzed via qPCR and histology. The cell surface receptor profile of HEF cells was examined by flow cytometry. HEF cells were also assayed through the collagen contraction assay. Prx1CreERT2GFP:R26RTdTomato MSC lineage-tracking mice were employed to identify EF MSCs in vivo. Results. HEF cell lines were obtained from all 10 patients in the study. Cells from 2/10 patients demonstrated full MSC potential, while cells from 6/10 patients demonstrated progenitor potential; 2/10 patients presented with cells that retained only adipogenic potential. HEF cells demonstrated MSC surface marker expression. All patient cell lines contracted collagen gels. A Prx1-positive population in mouse epidural fat that appeared to contribute to the dura of the spinal cord was observed in vivo. Conclusions. MSC and progenitor populations are present within HEF. MSCs were not identified in all patients examined in the current study. Furthermore, all patient lines demonstrated collagen contraction capacity, suggesting either a contaminating activated fibroblast population or HEF MSCs/progenitors also demonstrating a fibroblast-like phenotype. In vivo analysis suggests that these cell populations may contribute to the dura. Overall, these results suggest that cells within epidural fat may play a biological role within the local environment above providing a mechanical buffer.

Isolation and characterization of human gastric cell lines with stem cell phenotypes

2007 ◽  
Vol 22 (9) ◽  
pp. 1460-1468 ◽  
Author(s):  
Yuan-Chieh Yang ◽  
Sheng-Wen Wang ◽  
Hsiao-Yen Hung ◽  
Chia-Cheng Chang ◽  
I-Chen Wu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Gastric Cell ◽  
Isolation And Characterization ◽  
Cell Phenotypes

Characterization of stem cell–like cancer cells in immune-competent mice

Blood ◽  
2006 ◽  
Vol 108 (12) ◽  
pp. 3906-3912 ◽  
Author(s):  
Jorg A. Kruger ◽  
Charles D. Kaplan ◽  
Yunping Luo ◽  
He Zhou ◽  
Dorothy Markowitz ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cancer Cells ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Side Population ◽  
Carcinoma Cell Lines ◽  
Tumorigenic Potential ◽  
4T1 Cells

AbstractRecently, the cancer stem cell hypothesis has gained significant recognition as the descriptor of tumorigenesis. Although previous studies relied on transplanting human or rat tumor cells into immunecompromised mice, our study used the Hoechst 33342 dye–based side population (SP) technique to isolate and transplant stem cell–like cancer cells (SCLCCs) from the 4T1 and NXS2 murine carcinoma cell lines into the immune-competent microenvironment of syngeneic mice. 4T1 cells displayed an SP of 2% with a Sca-1highc-Kit–CD45– phenotype, whereas NXS2 cells contained an SP of 0.2% with a Sca-1highCD24highc-Kit–CD45–GD high2 phenotype. Reverse transcription–polymerase chain reaction (RT-PCR) further revealed up-regulation in SP cells of ABCG2, Sca-1, Wnt-1, and TGF-β2. Additionally, 4T1 and NXS2 SP cells exhibited increased resistance to chemotherapy, and 4T1 SP cells also showed an increased ability to efflux doxorubicin, which correlated with a selective increase in the percentage of SP cells found in the tumors of doxorubicin-treated mice. Most importantly, SP cells showed a markedly higher repopulation and tumorigenic potential in vivo, which correlated with an increased number of cells in the SP compartment of SP-derived tumors. Taken together, these results show that we successfully characterized SCLCCs from 2 murine carcinoma cell lines in the immune-competent microenvironment of syngeneic mice.

Isolation and characterization of conditionally immortalized astrocyte cell lines derived from adult human spinal cord

Glia ◽  
1994 ◽  
Vol 10 (3) ◽  
pp. 211-226 ◽  
Author(s):  
Scott R. Whittemore ◽  
Joseph T. Neary ◽  
Naomi Kleitman ◽  
Henry R. Sanon ◽  
Adelaida Benigno ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Cell Lines ◽  
Human Spinal Cord ◽  
Adult Human ◽  
Isolation And Characterization

Isolation and Characterization of Extracellular Vesicles in Stem Cell-Related Studies

2017 ◽  
pp. 205-223
Author(s):  
Zezhou Zhao ◽  
Dillon C. Muth ◽  
Vasiliki Mahairaki ◽  
Linzhao Cheng ◽  
Kenneth W. Witwer
Keyword(s):  
Stem Cell ◽  
Extracellular Vesicles ◽  
Isolation And Characterization
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