scholarly journals “Free” Enzymic Activity of Mitochondrial Fractions from Liver Tumours

1956 ◽  
Vol 10 (3) ◽  
pp. 587-593 ◽  
Author(s):  
E Reid ◽  
Margery A O'neal
Keyword(s):  
Enzymic Activity ◽  
Liver Tumours ◽  
Mitochondrial Fractions

Localization of oxalacetate keto–enol-tautomerase

1976 ◽  
Vol 54 (3) ◽  
pp. 233-237 ◽  
Author(s):  
James C. Wesenberg ◽  
Aravind Chaudhari ◽  
Robert G. Annett
Keyword(s):  
Enzymic Activity ◽  
The Other ◽  
Nuclear Fraction ◽  
Marker Enzymes ◽  
Animal Cells ◽  
Intracellular Location ◽  
Pig Kidney ◽  
Fractionation Procedure ◽  
Mitochondrial Fractions ◽  
Soluble Phase

The intracellular location of oxalacetate keto–enol-tautomerase (oxaloacetate keto–enol-isomerase) (EC 5.3.2.2) has been determined in two types of animal cells, rat liver and pig kidney. Two fractionation procedures were adopted and modified to suit each type of tissue. One fractionation procedure gave the soluble phase, microsomal and mitochondrial fractions, while the other isolated the nuclear fraction. The tautomerase is distributed among the soluble phase, microsomes and mitochondria in both tissues. Fractionation efficiency was checked by determining percentage recoveries of enzymic activity and total protein after each step, by microscopy studies and by determining the distribution of several marker enzymes.

Binding of Tissue Plasminogen Activator to Vascular Grafts

1989 ◽  
Vol 61 (01) ◽  
pp. 131-136 ◽  
Author(s):  
Richard A Harvey ◽  
Hugh C Kim ◽  
Jonathan Pincus ◽  
Stanley Z Trooskin ◽  
Josiah N Wilcox ◽  
...  
Keyword(s):  
Plasminogen Activator ◽  
Tissue Plasminogen Activator ◽  
Fibrinolytic Activity ◽  
Polymer Surface ◽  
Enzymic Activity ◽  
Vascular Prostheses ◽  
Chemically Modified ◽  
Insoluble Surfactant ◽  
Tissue Plasminogen

SummaryTissue plasminogen activator labeled with radioactive iodine (125I-tPA) was immobilized on vascular prostheses chemically modified with a thin coating of water-insoluble surfactant, tridodecylmethylammonium chloride (TDM AC). Surfactant- treated Dacron, polytetrafluoroethylene (PTFE), silastic, polyethylene and polyurethane bound appreciable amounts of 125I- tPA (5-30 μg 125I-tPA/cm2). Upon exposure to human plasma, the amount of 125I-tPA bound to the surface shows an initial drop during the first hour of incubation, followed by a slower, roughly exponential release with a t½ of appoximately 75 hours. Prostheses containing bound tPA show fibrinolytic activity as measured both by lysis of clots formed in vitro, and by hydrolysis of a synthetic polypeptide substrate. Prior to incubation in plasma, tPA bound to a polymer surface has an enzymic activity similar, if not identical to that of the native enzyme in buffered solution. However, exposure to plasma causes a decrease in the fibrinolytic activity of both bound tPA and enzyme released from the surface of the polymer. These data demonstrate that surfactant-treated prostheses can bind tPA, and that these chemically modified devices can act as a slow-release drug delivery system with the potential for reducing prosthesis-induced thromboembolism.

Our experience in the surgical treatment of metastatic liver tumours

2004 ◽  
Vol 42 (05) ◽  
Author(s):  
J Pósán ◽  
A Vágvölgyi ◽  
I Takács ◽  
A Furka ◽  
M Boland ◽  
...  
Keyword(s):  
Surgical Treatment ◽  
Liver Tumours ◽  
Metastatic Liver

Tight junction proteins in HCC and metastatic liver tumours

2005 ◽  
Vol 43 (05) ◽  
Author(s):  
Cs Páska ◽  
E Orbán ◽  
A Kiss ◽  
Zs Schaff ◽  
A Szijjártó ◽  
...  
Keyword(s):  
Tight Junction ◽  
Tight Junction Proteins ◽  
Liver Tumours ◽  
Metastatic Liver ◽  
Junction Proteins

Laparoscopic surgery of benign liver tumours

2015 ◽  
Vol 68 (1) ◽  
pp. 3-7 ◽  
Author(s):  
Péter Kupcsulik ◽  
Oszkár Hahn ◽  
Attila Szíjártó ◽  
Attila Zsirka ◽  
Tamás Winternitz ◽  
...  
Keyword(s):  
Laparoscopic Surgery ◽  
Liver Tumours ◽  
Benign Liver

Munkahelyünkön 2004–2014 között 273 elektív műtét történt benignus májelváltozás miatt. 83 esetben laparoszkópos (LAP) beavatkozásra került sor: cystafenestratio 52, májresectio 31 esetben történt. A LAP resectiókat hasonló demográfiai és klinikai jellemzőkkel bíró betegek nyitott műtéteivel összehasonlítva megállapítható, hogy a műtéti idő a LAP csoportban (113,7 perc) szignifikánsan hosszabb volt, mint a nyitott műtéteknél (89,5 perc), a kórházi ápolási idő viszont rövidebb (5,8 vs 9,1 nap). Posztoperatív szövődmény a LAP csoportban nem volt, nyitott műtétek után két sebgennyedés, egy UH-vezérelt drainezést igénylő epegyülem fordult elő. A nyitott csoportban 4, a LAP csoportban 3 beteg igényelt transzfúziót. Műtéti halálozás nem volt, reoperációra nem került sor. A közleményben részletezett műtéti technika biztonságos resectiót tesz lehetővé a máj nehezen hozzáférhető, 7–8. szegmentuma területén is. Az eredmények alapján a LAP májresectio megfelelő preoperatív vizsgálatok birtokában választható módszer májdaganatok sebészi kezelésére.

Magnetic Resonance Imaging of Uncommon Hepatic Mesenchymal Tumours: Haemangioendothelioma and Angiosarcoma

2019 ◽  
Vol 15 (4) ◽  
pp. 362-368
Author(s):  
Andrzej Cieszanowski ◽  
Agnieszka Anysz-Grodzicka ◽  
Joanna Podgorska ◽  
Beata Jagielska ◽  
Jakub Pałucki
Keyword(s):  
Contrast Enhancement ◽  
Correct Diagnosis ◽  
Malignant Potential ◽  
Hepatobiliary Phase ◽  
Imaging Features ◽  
Liver Tumours ◽  
Aged Woman ◽  
High Apparent Diffusion Coefficient ◽  
Adc Values ◽  
Mesenchymal Tumours

<P>Background: Primary Hepatic Epithelioid Haemangioendothelioma (HEHE) and Primary Hepatic Angiosarcoma (PHA) are rare mesenchymal tumours with different malignant potential. Whereas HEHE demonstrates low to intermediate malignant potential, PHA is an aggressive malignancy with poor prognosis. The knowledge of typical imaging features of these lesions may facilitate correct diagnosis; however, the ultimate diagnosis of HEHE and PHA is based on histopathologic examination. </P><P> Discussion: The most typical findings helpful in diagnosing HEHE are: Presence of multiple, confluent nodules located at the liver periphery (in young to middle-aged woman), retraction of the liver capsule, marked hyperintensity on T2-weighted images, “target-sign” appearance, progressive centripetal contrast enhancement, and relatively high Apparent Diffusion Coefficient (ADC) values. More than &#8805;50% of nodules are hyper- or isointense on Hepatobiliary Phase (HBP) images. Conclusion: The imaging features suggestive of PHA are: Occurrence of metastases (lungs, spleen) at the time of diagnosis, presence of a large dominant mass with smaller satellites, heterogeneity and areas of haemorrhage in a dominant mass, progressive contrast enhancement, slightly elevated ADC values as compared to other malignant liver tumours.</P>

Purification of Glycerol Kinase by "Dye-Ligand" Chromatography and Hydrophobic Interaction Chromatography on Bead-Cellulose Derivatives

1993 ◽  
Vol 58 (2) ◽  
pp. 445-451 ◽  
Author(s):  
Vladimír Žúbor ◽  
Albert Breier ◽  
Marta Horváthová ◽  
Dagmar Hagarová ◽  
Peter Gemeiner ◽  
...  
Keyword(s):  
Hydrophobic Interaction ◽  
Specific Activity ◽  
Hydrophobic Interaction Chromatography ◽  
Glycerol Kinase ◽  
Enzymic Activity ◽  
Cellulose Derivatives ◽  
Long Term Storage ◽  
Bead Cellulose ◽  
Term Storage

The crude extract of cytosole enzymes was obtained from homogenized cells of Saccharomyces cerevisiae by partition. The enzyme was then isolated from the lower aqueous phase displaying higher glycerol kinase activity by dye-ligand chromatography on Cibacron Blue (CB) or Remazol Brilliant Blue R (RB)-derivatized bead-cellulose, ATP being the eluent. The specific activity of glycerol kinase rised more than 10 and 7-times after affinity dye-ligand chromatography and hydrophobic interaction chromatography, respectively. Glycerol kinase obtained by the latter method was purified by CB-bead cellulose. The final preparation maintained its enzymic activity without noticeable losses during a long-term storage at 4 °C in dark.

scholarly journals 466 Resection or Enucleation for Extremely Giant Hepatic Haemangiomata? A Case Report and Review of The Literature

2021 ◽  
Vol 108 (Supplement_2) ◽  
Author(s):  
R E Fraser ◽  
G R Layton ◽  
L L Kuan ◽  
A R Dennison
Keyword(s):  
Expectant Management ◽  
Evidence Base ◽  
Primary Treatment ◽  
Cavernous Haemangioma ◽  
Liver Tumours ◽  
Case Presentation ◽  
Best Management ◽  
Anatomical Liver Resection ◽  
Benign Liver

Abstract Background Cavernous hepatic haemangiomas are benign liver tumours and although common when small, giant haemangiomas (usually accepted as being greater than 10cm) are infrequent. Treatment is indicated in patients who are symptomatic or if diagnosis is unclear, although with giant haemangiomas, many support expectant management of asymptomatic lesions due to the risk of major complications. Traditionally hepatic resection has been the primary treatment option for these lesions, but a variety of other techniques, including enucleation, have been described as safe and effective alternatives. There remains equipoise in respect of the best management of giant haemangiomas above 10cm. Cases of such size are rare and so there is a paucity of data available. Case presentation We present a case of a 65-year-old male who underwent successful anatomical liver resection for a 5kg giant cavernous haemangioma of 26cm diameter following its incidental identification during an ultrasound scan. We also discuss and compare the role of resection and enucleation for the treatment of haemangiomata greater than 20cm in diameter. Conclusions This case demonstrates successful resection of an unusually giant haemangioma which, in contrast to the majority of literature, provides a valuable addition to the limited evidence base for management of this condition by anatomical resection.

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