scholarly journals Over-production of nitric oxide by oxidative stress-induced activation of the TGF-β1/PI3K/Akt pathway in mesangial cells cultured in high glucose

2013 ◽  
Vol 34 (4) ◽  
pp. 507-514 ◽  
Author(s):  
Yun-peng Zhai ◽  
Qian Lu ◽  
Yao-wu Liu ◽  
Qian Cheng ◽  
Ya-qin Wei ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
High Glucose ◽  
Mesangial Cells ◽  
Akt Pathway ◽  
Tgf Β1 ◽  
Cells Cultured

Berberine inhibits aldose reductase and oxidative stress in rat mesangial cells cultured under high glucose

2008 ◽  
Vol 475 (2) ◽  
pp. 128-134 ◽  
Author(s):  
Weihua Liu ◽  
Peiqinq Liu ◽  
Sha Tao ◽  
Yanhui Deng ◽  
Xuejuan Li ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Aldose Reductase ◽  
High Glucose ◽  
Mesangial Cells ◽  
And Oxidative Stress ◽  
Cells Cultured

SDF-1α reduces fibronectin expression in rat mesangial cells induced by TGF-β1 and high glucose through PI3K/Akt pathway

2013 ◽  
Vol 319 (12) ◽  
pp. 1796-1803 ◽  
Author(s):  
Dan Zhang ◽  
Shiying Shao ◽  
Hongxia Shuai ◽  
Yunchuan Ding ◽  
Wei Shi ◽  
...  
Keyword(s):  
High Glucose ◽  
Mesangial Cells ◽  
Akt Pathway ◽  
Fibronectin Expression ◽  
Tgf Β1

scholarly journals Circ-ACTR2 aggravates the high glucose-induced cell dysfunction of human renal mesangial cells through mediating the miR-205-5p/HMGA2 axis in diabetic nephropathy

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Jie Yun ◽  
Jinyu Ren ◽  
Yufei Liu ◽  
Lijuan Dai ◽  
Liqun Song ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetic Nephropathy ◽  
High Glucose ◽  
Cell Injury ◽  
Mesangial Cells ◽  
Protein Detection ◽  
Cell Counting ◽  
Luciferase Reporter ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cell Dysfunction

Abstract Background Circular RNAs (circRNAs) have been considered as pivotal biomarkers in Diabetic nephropathy (DN). CircRNA ARP2 actin-related protein 2 homolog (circ-ACTR2) could promote the HG-induced cell injury in DN. However, how circ-ACTR2 acts in DN is still unclear. This study aimed to explore the molecular mechanism of circ-ACTR2 in DN progression, intending to provide support for the diagnostic and therapeutic potentials of circ-ACTR2 in DN. Methods RNA expression analysis was conducted by the quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Cell growth was measured via Cell Counting Kit-8 and EdU assays. Inflammatory response was assessed by Enzyme-linked immunosorbent assay. The protein detection was performed via western blot. Oxidative stress was evaluated by the commercial kits. The molecular interaction was affirmed through dual-luciferase reporter and RNA immunoprecipitation assays. Results Circ-ACTR2 level was upregulated in DN samples and high glucose (HG)-treated human renal mesangial cells (HRMCs). Silencing the circ-ACTR2 expression partly abolished the HG-induced cell proliferation, inflammation and extracellular matrix accumulation and oxidative stress in HRMCs. Circ-ACTR2 was confirmed as a sponge for miR-205-5p. Circ-ACTR2 regulated the effects of HG on HRMCs by targeting miR-205-5p. MiR-205-5p directly targeted high-mobility group AT-hook 2 (HMGA2), and HMGA2 downregulation also protected against cell injury in HG-treated HRMCs. HG-mediated cell dysfunction was repressed by miR-205-5p/HMGA2 axis. Moreover, circ-ACTR2 increased the expression of HMGA2 through the sponge effect on miR-205-5p in HG-treated HRMCs. Conclusion All data have manifested that circ-ACTR2 contributed to the HG-induced DN progression in HRMCs by the mediation of miR-205-5p/HMGA2 axis.

scholarly journals Effects of Qijin granules on high glucose-induced proliferation, apoptosis and expression of nuclear factor- κB and MCP-1 in rat glomerular mesangial cells

2021 ◽  
Vol 20 (9) ◽  
pp. 1819-1826
Author(s):  
Yuanfeng Yang ◽  
Gaocai Xiong ◽  
Renhui Yang ◽  
Yuchuan Li ◽  
Yuling Luo ◽  
...  
Keyword(s):  
Cell Cycle ◽  
High Glucose ◽  
Mesangial Cells ◽  
Control Group ◽  
Glomerular Mesangial Cells ◽  
Smad Signaling ◽  
Apoptosis Rate ◽  
Proliferation And Apoptosis ◽  
Protein Expressions ◽  
Tgf Β1

Purpose: To investigate the effects of Qijin granules on high glucose-induced proliferation and apoptosis in rat glomerular mesangial cells (MC).Methods: MC cells from rats were passaged and cultured, and randomly divided into control group (CNG), high glucose group (HGG), Western medicine group (WMG, high glucose + Benazepril + Gliquidone), and Qijin granules 1/2/3 group (high glucose + different doses of Qijin granules). Mesangial cells proliferation was measured using MTT assay. The NF-κB, MCP-1 and inflammatory factors in supernatant were determined by ELISA. Apoptosis rate and cell cycle were assessed by flow cytometry. The apoptosis-related TGF-β1/Smad signaling pathway-related protein expressions were measured by Western blot.Results: The A-value and early apoptosis rate, apoptosis rate and S-phase percentage, and protein expressions of NF-κB, MCP-1, IL-6, IL-2, TNF-ɑ, Bax, Cyt-C, caspase-3, TGF-β1, and p-Smad3 of MC cells in the HGG at 12 h, 24 h and 48 h were higher than those in the CNG. The above indices were lower in the WMG, and Qijin granules 1/2/3 groups than in the HGG. The Bcl-2, Smad7 protein expression level and the percentage of G1 and G2/M phase were lower in the HGG than in the CNG, and the above indeices were higher in the WMG and Qijin granules 1/2/3 group than in HGG.Conclusion: Qijin granules can dose-dependently inhibit high glucose-induced proliferation and apoptosis in rat MC cells, block the cell cycle and reduce inflammatory responses. This may be related to the regulation of NF-κB, MCP-1 and TGF-β1/Smad signaling pathways. These findings provide theoretical and experimental basis for the clinical treatment of early diabetic nephropathy.

scholarly journals The Inhibitory Effect of siRNAs on The High Glucose-Induced Overexpression of TGF-β1 in Mesangial Cells

2006 ◽  
Vol 21 (3) ◽  
pp. 430 ◽  
Author(s):  
Hey-Jeong Noh ◽  
Hyun-Chul Kim ◽  
Sang-Sook Lee ◽  
Yu-Na Kang ◽  
Young-Mi Chae ◽  
...  
Keyword(s):  
High Glucose ◽  
Mesangial Cells ◽  
Inhibitory Effect ◽  
Tgf Β1

scholarly journals High glucose and TGF-β1 reduce expression of endoplasmic reticulum-resident selenoprotein S and selenoprotein N in human mesangial cells

Renal Failure ◽  
2019 ◽  
Vol 41 (1) ◽  
pp. 762-769
Author(s):  
Fumeng Huang ◽  
Yuanxu Guo ◽  
Li Wang ◽  
Lanmei Jing ◽  
Zhao Chen ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
High Glucose ◽  
Mesangial Cells ◽  
Human Mesangial Cells ◽  
Selenoprotein S ◽  
Tgf Β1
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