Berberine inhibits aldose reductase and oxidative stress in rat mesangial cells cultured under high glucose

2008 ◽  
Vol 475 (2) ◽  
pp. 128-134 ◽  
Author(s):  
Weihua Liu ◽  
Peiqinq Liu ◽  
Sha Tao ◽  
Yanhui Deng ◽  
Xuejuan Li ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Aldose Reductase ◽  
High Glucose ◽  
Mesangial Cells ◽  
And Oxidative Stress ◽  
Cells Cultured

An analysis of high glucose and glucosamine-induced gene expression and oxidative stress in renal mesangial cells

2006 ◽  
Vol 112 (4-5) ◽  
pp. 189-218 ◽  
Author(s):  
Davis W. Cheng ◽  
Yan Jiang ◽  
Anath Shalev ◽  
Renu Kowluru ◽  
Errol D. Crook ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
High Glucose ◽  
Mesangial Cells ◽  
And Oxidative Stress

FOXP1 inhibits high glucose-induced ECM accumulation and oxidative stress in mesangial cells

2019 ◽  
Vol 313 ◽  
pp. 108818 ◽  
Author(s):  
Heli Xiang ◽  
Wujun Xue ◽  
Xiaoyan Wu ◽  
Jin Zheng ◽  
Chenguang Ding ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
High Glucose ◽  
Mesangial Cells ◽  
And Oxidative Stress

scholarly journals Over-production of nitric oxide by oxidative stress-induced activation of the TGF-β1/PI3K/Akt pathway in mesangial cells cultured in high glucose

2013 ◽  
Vol 34 (4) ◽  
pp. 507-514 ◽  
Author(s):  
Yun-peng Zhai ◽  
Qian Lu ◽  
Yao-wu Liu ◽  
Qian Cheng ◽  
Ya-qin Wei ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
High Glucose ◽  
Mesangial Cells ◽  
Akt Pathway ◽  
Tgf Β1 ◽  
Cells Cultured

scholarly journals Circ-ACTR2 aggravates the high glucose-induced cell dysfunction of human renal mesangial cells through mediating the miR-205-5p/HMGA2 axis in diabetic nephropathy

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Jie Yun ◽  
Jinyu Ren ◽  
Yufei Liu ◽  
Lijuan Dai ◽  
Liqun Song ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetic Nephropathy ◽  
High Glucose ◽  
Cell Injury ◽  
Mesangial Cells ◽  
Protein Detection ◽  
Cell Counting ◽  
Luciferase Reporter ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cell Dysfunction

Abstract Background Circular RNAs (circRNAs) have been considered as pivotal biomarkers in Diabetic nephropathy (DN). CircRNA ARP2 actin-related protein 2 homolog (circ-ACTR2) could promote the HG-induced cell injury in DN. However, how circ-ACTR2 acts in DN is still unclear. This study aimed to explore the molecular mechanism of circ-ACTR2 in DN progression, intending to provide support for the diagnostic and therapeutic potentials of circ-ACTR2 in DN. Methods RNA expression analysis was conducted by the quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Cell growth was measured via Cell Counting Kit-8 and EdU assays. Inflammatory response was assessed by Enzyme-linked immunosorbent assay. The protein detection was performed via western blot. Oxidative stress was evaluated by the commercial kits. The molecular interaction was affirmed through dual-luciferase reporter and RNA immunoprecipitation assays. Results Circ-ACTR2 level was upregulated in DN samples and high glucose (HG)-treated human renal mesangial cells (HRMCs). Silencing the circ-ACTR2 expression partly abolished the HG-induced cell proliferation, inflammation and extracellular matrix accumulation and oxidative stress in HRMCs. Circ-ACTR2 was confirmed as a sponge for miR-205-5p. Circ-ACTR2 regulated the effects of HG on HRMCs by targeting miR-205-5p. MiR-205-5p directly targeted high-mobility group AT-hook 2 (HMGA2), and HMGA2 downregulation also protected against cell injury in HG-treated HRMCs. HG-mediated cell dysfunction was repressed by miR-205-5p/HMGA2 axis. Moreover, circ-ACTR2 increased the expression of HMGA2 through the sponge effect on miR-205-5p in HG-treated HRMCs. Conclusion All data have manifested that circ-ACTR2 contributed to the HG-induced DN progression in HRMCs by the mediation of miR-205-5p/HMGA2 axis.

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