scholarly journals In vivo high-efficiency targeted photodynamic therapy of ultra-small Fe3O4@polymer-NPO/PEG-Glc@Ce6 nanoprobes based on small size effect

2017 ◽  
Vol 9 (5) ◽  
pp. e383-e383 ◽  
Author(s):  
Ting Yin ◽  
Qian Zhang ◽  
Haigang Wu ◽  
Guo Gao ◽  
Joseph G Shapter ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Size Effect ◽  
High Efficiency ◽  
Targeted Photodynamic Therapy

Protease-Activable Cell-Penetrating Peptide–Protoporphyrin Conjugate for Targeted Photodynamic Therapy in Vivo

2015 ◽  
Vol 7 (51) ◽  
pp. 28319-28329 ◽  
Author(s):  
Shi-Ying Li ◽  
Hong Cheng ◽  
Wen-Xiu Qiu ◽  
Li-Han Liu ◽  
Si Chen ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
Targeted Photodynamic Therapy

Folic Acid–Conjugated Pyropheophorbide a as the Photosensitizer Tested for In Vivo Targeted Photodynamic Therapy

2017 ◽  
Vol 106 (6) ◽  
pp. 1482-1489 ◽  
Author(s):  
Jin Wang ◽  
Qian Liu ◽  
Yuting Zhang ◽  
Huan Shi ◽  
Hui Liu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Folic Acid ◽  
Pyropheophorbide A ◽  
Targeted Photodynamic Therapy

scholarly journals Targeted photodynamic therapy selectively kills activated fibroblasts in experimental arthritis

Rheumatology ◽  
2020 ◽  
Vol 59 (12) ◽  
pp. 3952-3960 ◽  
Author(s):  
Daphne N Dorst ◽  
Mark Rijpkema ◽  
Marti Boss ◽  
Birgitte Walgreen ◽  
Monique M A Helsen ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Therapeutic Potential ◽  
Ex Vivo ◽  
Synovial Fibroblasts ◽  
Knee Joints ◽  
Specific Cell ◽  
Collagen Induced Arthritis ◽  
Targeted Photodynamic Therapy

Abstract Objective In RA, synovial fibroblasts become activated. These cells express fibroblast activation protein (FAP) and contribute to the pathogenesis by producing cytokines, chemokines and proteases. Selective depletion in inflamed joints could therefore constitute a viable treatment option. To this end, we developed and tested a new therapeutic strategy based on the selective destruction of FAP-positive cells by targeted photodynamic therapy (tPDT) using the anti-FAP antibody 28H1 coupled to the photosensitizer IRDye700DX. Methods After conjugation of IRDye700DX to 28H1, the immunoreactive binding and specificity of the conjugate were determined. Subsequently, tPDT efficiency was established in vitro using a 3T3 cell line stably transfected with FAP. The biodistribution of [111In]In-DTPA-28H1 with and without IRDye700DX was assessed in healthy C57BL/6N mice and in C57BL/6N mice with antigen-induced arthritis. The potential of FAP-tPDT to induce targeted damage was determined ex vivo by treating knee joints from C57BL/6N mice with antigen-induced arthritis 24 h after injection of the conjugate. Finally, the effect of FAP-tPDT on arthritis development was determined in mice with collagen-induced arthritis. Results 28H1-700DX was able to efficiently induce FAP-specific cell death in vitro. Accumulation of the anti-FAP antibody in arthritic knee joints was not affected by conjugation with the photosensitizer. Arthritis development was moderately delayed in mice with collagen-induced arthritis after FAP-tPDT. Conclusion Here we demonstrate the feasibility of tPDT to selectively target and kill FAP-positive fibroblasts in vitro and modulate arthritis in vivo using a mouse model of RA. This approach may have therapeutic potential in (refractory) arthritis.

scholarly journals Carcinoembryonic antigen-targeted photodynamic therapy in colorectal cancer models

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Fortuné M. K. Elekonawo ◽  
Desirée L. Bos ◽  
David M. Goldenberg ◽  
Otto C. Boerman ◽  
Mark Rijpkema
Keyword(s):  
Colorectal Cancer ◽  
Photodynamic Therapy ◽  
Tumor Growth ◽  
Carcinoembryonic Antigen ◽  
Tumor Growth Rate ◽  
Incomplete Resection ◽  
Radiant Exposure ◽  
Antibody Conjugate ◽  
Targeted Photodynamic Therapy

Abstract Background In colorectal cancer, survival of patients is drastically reduced when complete resection is hampered by involvement of critical structures. Targeted photodynamic therapy (tPDT) is a local and targeted therapy which could play a role in eradicating residual tumor cells after incomplete resection. Since carcinoembryonic antigen (CEA; CEACAM5) is abundantly overexpressed in colorectal cancer, it is a potential target for tPDT of colorectal cancer. Methods To address the potential of CEA-targeted PDT, we compared colorectal cancer cell lines with different CEA-expression levels (SW-48, SW-480, SW-620, SW-1222, WiDr, HT-29, DLD-1, LS174T, and LoVo) under identical experimental conditions. We evaluated the susceptibility to tPDT by varying radiant exposure and concentration of our antibody conjugate (DTPA-hMN-14-IRDye700DX). Finally, we assessed the efficacy of tPDT in vivo in 18 mice (BALB/cAnNRj-Foxn1nu/nu) with subcutaneously xenografted LoVo tumors. Results In vitro, the treatment effect of tPDT varied per cell line and was dependent on both radiant exposure and antibody concentration. Under standardized conditions (94.5 J/cm2 and 0.5 μg/μL antibody conjugate concentration), the effect of tPDT was higher in cells with higher CEA availability: SW-1222, LS174T, LoVo, and SW-48 (22.8%, 52.8%, 49.9%, and 51.9% reduction of viable cells, respectively) compared to cells with lower CEA availability. Compared to control groups (light or antibody conjugate only), tumor growth rate was reduced in mice with s.c. LoVo tumors receiving tPDT. Conclusion Our findings suggest cells (and tumors) have different levels of susceptibility for tPDT even though they all express CEA. Furthermore, tPDT can effectively reduce tumor growth in vivo.

A pH-responsive stellate mesoporous silica based nanophotosensitizer for in vivo cancer diagnosis and targeted photodynamic therapy

2019 ◽  
Vol 7 (1) ◽  
pp. 211-219 ◽  
Author(s):  
Ai-Lan Lin ◽  
Song-Zi Li ◽  
Cai-Hong Xu ◽  
Xing-Shu Li ◽  
Bi-Yuan Zheng ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Mesoporous Silica ◽  
Cancer Diagnosis ◽  
Tumor Site ◽  
Ph Responsive ◽  
Targeted Photodynamic Therapy

A novel stellate mesoporous silica-based pH-responsive nanophotosensitizer can be localized and specifically activated at tumor site for cancer diagnosis and targeted PDT.

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