Protease-Activable Cell-Penetrating Peptide–Protoporphyrin Conjugate for Targeted Photodynamic Therapy in Vivo

2015 ◽  
Vol 7 (51) ◽  
pp. 28319-28329 ◽  
Author(s):  
Shi-Ying Li ◽  
Hong Cheng ◽  
Wen-Xiu Qiu ◽  
Li-Han Liu ◽  
Si Chen ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
Targeted Photodynamic Therapy

Cell-Penetrating Peptide-Mediated Nanovaccine Delivery

2021 ◽  
Vol 22 ◽  
Author(s):  
Jizong Jiang
Keyword(s):  
Immune System ◽  
Immune Responses ◽  
Cell Penetrating Peptide ◽  
Antigen Delivery ◽  
Efficient Manner ◽  
Antigen Uptake ◽  
Cell Penetrating ◽  
The Stability ◽  
Antigen Presenting

Abstract: Vaccination with small antigens, such as proteins, peptides, or nucleic acids, is used to activate the immune system and trigger the protective immune responses against a pathogen. Currently, nanovaccines are undergoing development instead of conventional vaccines. The size of nanovaccines is in the range of 10–500 nm, which enables them to be readily taken up by cells and exhibit improved safety profiles. However, low-level immune responses, as the removal of redundant pathogens, trigger counter-effective activation of the immune system invalidly and present a challenging obstacle to antigen recognition and its uptake via antigen-presenting cells (APCs). In addition, toxicity can be substantial. To overcome these problems, a variety of cell-penetrating peptide (CPP)-mediated vaccine delivery systems based on nanotechnology have been proposed, most of which are designed to improve the stability of antigens in vivo and their delivery into immune cells. CPPs are particularly attractive components of antigen delivery. Thus, the unique translocation property of CPPs ensures that they remain an attractive carrier with the capacity to deliver cargo in an efficient manner for the application of drugs, gene transfer, protein, and DNA/RNA vaccination delivery. CPP-mediated nanovaccines can enhance antigen uptake, processing, and presentation by APCs, which are the fundamental steps in initiating an immune response. This review describes the different types of CPP-based nanovaccines delivery strategies.

scholarly journals Cell-Penetrating Peptide Enhanced Intracellular Raman Imaging and Photodynamic Therapy

2013 ◽  
Vol 10 (6) ◽  
pp. 2291-2298 ◽  
Author(s):  
Andrew M. Fales ◽  
Hsiangkuo Yuan ◽  
Tuan Vo-Dinh
Keyword(s):  
Photodynamic Therapy ◽  
Raman Imaging ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating

scholarly journals Effective In Vivo Topical Delivery of siRNA and Gene Silencing in Intact Corneal Epithelium Using a Modified Cell-Penetrating Peptide

2019 ◽  
Vol 17 ◽  
pp. 891-906 ◽  
Author(s):  
Davide Schiroli ◽  
María J. Gómara ◽  
Eleonora Maurizi ◽  
Sarah D. Atkinson ◽  
Laura Mairs ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Corneal Epithelium ◽  
Topical Delivery ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating

scholarly journals A212 CHROMOFUNGIN PROTECTS AGAINST DSS-INDUCED COLITIS BY REGULATING P-53 APOPTITIC PATHWAY

2020 ◽  
Vol 3 (Supplement_1) ◽  
pp. 84-85
Author(s):  
A Diarra ◽  
N Eissa ◽  
J Ghia
Keyword(s):  
B Cell Lymphoma ◽  
Peritoneal Macrophages ◽  
Cell Penetrating Peptide ◽  
Peptide Motif ◽  
Apoptotic Pathway ◽  
Apoptosis Pathway ◽  
Cell Penetrating ◽  
Vehicle Treatment ◽  
A Cell

Abstract Background Development of ulcerative colitis is associated with epithelial apoptosis mediated by p53-apoptotic pathway through the activation B-cell lymphoma 2 (Bcl-2), Bcl-2 associated-X protein (BAX) and Bcl-2 antagonist/killer-1 (BAK1) proteins. Chromofungin (CHR), a chromogranin-A derived peptide expressing a cell penetrating peptide motif, decreased the severity of colitis via the suppression of mucosal and pro-inflammatory macrophages-related p53-dependent apoptosis. Aims We aimed to investigate a) whether the gene profile expression of apoptosis could be extended to other p53-associated genes; b) whether the gene expression of some of the p53-apoptosis marker could be confirmed by protein analysis; and c) whether due to the cell penetrating peptide motif, CHR could enter into peritoneal macrophages. Methods UC-related colitis was induced in C57BL/6 mice (7 weeks) by administering dextran sulfate sodium (DSS) (5%, 5 days). Preventive CHR (2.5 mg/kg/day) or vehicle treatment started 1-day before colitis induction and lasted for 5-days. Profiler™ PCR Array was performed to screen a panel of 84 genes representative of the p53 signal pathway in colitic whole mucosa distal colonic samples treated or not with CHR. Western blot analysis was performed to confirm individual protein changes. Naïve macrophages were plated overnight and nonadherent cells were removed the next day. Cells were incubated with rhodamined CHR (4 ul) for 5, 10, 20, 30 min before washing and fixing them, detection was made via confocal microscopy. Results In colitic conditions, an up regulation of 26 genes associated to the p53-dependent apoptosis pathway were detected including Apaf1, Bax, Bbc3, Bcl2, Cradd, Fadd, Cul9, Pmaip1, Tnfrsf10b. In vivo CHR treatment decreased significantly the colitis and was associated with a significant downregulation of 19 genes including the 9 aforementioned when compared with biopsies from colitic groups. Compared to untreated groups, colitic mice treated with CHR demonstrated a significant decrease of BAX and BAK protein and the apoptotic ER stress inducer marker, X-Binding Protein 1. A large number of peritoneal macrophages displayed rhodamine within the all intracellular compartment. The presence of the peptide inside the cell can be visible as early as 5 min and the signal gradually increases. Conclusions CHR decreases the inflammatory process via the suppression of a large number of p53-related apoptotic proteins. CHR quickly enters the macrophage but the exact mechanism of entrance needs to be further defined. Targeting functional analysis of CHR may lead in the future to novel therapeutics for UC. Funding Agencies CCCNSERC

An amphipathic cell penetrating peptide aids cell penetration of cyclosporin A and increases its therapeutic effect in an in vivo mouse model for dry eye disease

2019 ◽  
Vol 55 (91) ◽  
pp. 13657-13660
Author(s):  
Soonsil Hyun ◽  
Lan Li ◽  
Kyung Chul Yoon ◽  
Jaehoon Yu
Keyword(s):  
Mouse Model ◽  
Cyclosporin A ◽  
Dry Eye ◽  
Cyclosporine A ◽  
Therapeutic Efficacy ◽  
Eye Disease ◽  
Cell Penetrating Peptide ◽  
Cell Penetration ◽  
Cell Penetrating

The use of CPP, LK-3, increases the cell penetration of cyclosporine A (CsA) at nanomolar concentrations and therapeutic efficacy.

scholarly journals The anticancer efficacy of paclitaxel liposomes modified with low-toxicity hydrophobic cell-penetrating peptides in breast cancer: an in vitro and in vivo evaluation

RSC Advances ◽  
2018 ◽  
Vol 8 (43) ◽  
pp. 24084-24093 ◽  
Author(s):  
Qi Zhang ◽  
Jing Wang ◽  
Hao Zhang ◽  
Dan Liu ◽  
Linlin Ming ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Targeted Delivery ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating Peptide ◽  
In Vivo Evaluation ◽  
Anticancer Efficacy ◽  
Cell Penetrating ◽  
Low Toxicity

Hydrophobic cell penetrating peptide PFVYLI-modified liposomes have been developed for the targeted delivery of PTX into tumors.

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