The cytoplasmic droplet may be indicative of sperm motility and normal spermiogenesis

Hui Xu; Shui-Qiao Yuan; Zhi-Hong Zheng; Wei Yan

doi:10.1038/aja.2013.69

Physical and morphological characteristics of the first three jets of Pêga jackasses sperm-rich fraction

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/1678-4162-7891 ◽

2016 ◽

Vol 68 (4) ◽

pp. 845-852

Author(s):

L.E. Carvalho ◽

J.M. Silva Filho ◽

M.S. Palhares ◽

A.L.R. Sales ◽

A.T. Gonczarowska ◽

...

Keyword(s):

Sperm Motility ◽

Sperm Morphology ◽

Morphological Characteristics ◽

Open Model ◽

Cytoplasmic Droplet ◽

The Third ◽

Semen Collection ◽

Normal Sperm ◽

Artificial Vagina

ABSTRACT The first three jets of the sperm-rich fraction of Pêga jackasses were collected and assessed separately. Five fertile Pêga jackasses were used as semen donors and underwent fractionated semen collection, using an open model artificial vagina. The first three jets of the semen were collected separately and assessed for volume, sperm motility, vigor, concentration/mL of semen, and sperm morphology. These characteristics were compared between first, second and third jets and between jackasses. It was observed that the jet volume differed (P<0.05) between jackasses, although it was similar (P>0.05) between first, second and third jets. Sperm motility did not differ (P>0.05) between jets and jackasses. Vigor was similar (P>0.05) between jets of the same jackass, and only the first jet differed (P<0.05) between jackasses. The first, second and third jets of the sperm-rich fraction had decreased sperm concentrations (P<0.05) of 955.56, 725.56 and 280.56x 106 sperm/mL of semen, respectively. Sperm morphology differed between the first three jets only for the incidence of mid-piece defect, higher in the third one (4.26%), compared to the first (3.36%) and second (3.38%) ones. When comparing the morphological characteristics of the sperm-rich fraction between five jackasses, regardless of the jet, there were differences in the percentage of normal sperm, proximal cytoplasmic droplet, mid-piece and head defects.

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Arrangement of Mitochondria in Spermatozoa of the Mexican Axolotl, Ambystoma Mexicanum

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010007357x ◽

1973 ◽

Vol 31 ◽

pp. 626-627

Author(s):

Ezzatollah Keyhani ◽

Larry F. Lemanski ◽

Sharon L. Lemanski

Keyword(s):

Plasma Membrane ◽

Sperm Motility ◽

Substrate Oxidation ◽

Ambystoma Mexicanum ◽

Axial Filament ◽

Mexican Axolotl ◽

Middle Piece

Energy for sperm motility is provided by both glycolytic and respiratory pathways. Mitochondria are involved in the latter pathway and conserve energy of substrate oxidation by coupling to phosphorylation. During spermatogenesis, the mitochondria undergo extensive transformation which in many species leads to the formation of a nebemkem. The nebemkem subsequently forms into a helix around the axial filament complex in the middle piece of spermatozoa.Immature spermatozoa of axolotls contain numerous small spherical mitochondria which are randomly distributed throughout the cytoplasm (Fig. 1). As maturation progresses, the mitochondria appear to migrate to the middle piece region where they become tightly packed to form a crystalline-like sheath. The cytoplasm in this region is no longer abundant (Fig. 2) and the plasma membrane is now closely apposed to the outside of the mitochondrial layer.

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Interactive effects of ocean warming and acidification on sperm motility and fertilization in the mussel Mytilus galloprovincialis

Marine Ecology Progress Series ◽

10.3354/meps11944 ◽

2016 ◽

Vol 562 ◽

pp. 101-111 ◽

Cited By ~ 12

Author(s):

AR Eads ◽

WJ Kennington ◽

JP Evans

Keyword(s):

Sperm Motility ◽

Mytilus Galloprovincialis ◽

Ocean Warming ◽

Interactive Effects

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Kriopreservasi Semen Kambing Boer dengan Konsentrasi Pengencer Nira Aren dan Gliserol Berbeda

Jurnal Ilmu dan Teknologi Peternakan Tropis ◽

10.33772/jitro.v6i1.5422 ◽

2019 ◽

Vol 6 (1) ◽

pp. 1

Author(s):

Muhammad Riyadhi ◽

Anis Wahdi ◽

Muhammad Rizal

Keyword(s):

Sperm Motility ◽

Membrane Integrity ◽

Egg Yolk ◽

Boer Goat ◽

Palm Juice ◽

Sperm Membrane ◽

Live Sperm

ABSTRAK Penelitian bertujuan untuk mengetahui efektivitas nira aren sebagai pengencer alternatif dalam proses pembekuan (kriopreservasi) semen kambing boer.Kriopreservasi semen kambing boer menggunakan pengencer tris-gliserol-kuning telur (P1 73-7-20%), nira aren-gliseol-kuning telur(masing-masing P2 74-6-20%, P3 73-7-20%, dan P4 72-8-20%) dan andromed (P5 tanpa mengandung kuning telur dan gliserol). Parameter evaluasi meliputi motilitas, viabilitas, dan membrane plasma utuh setelah pengenceran, ekuilibrasi dan thawing. Evaluasi motilitas pasca thawing menunjukkan P5 52% berbeda nyata (P<0.05) dengan P1 42%, selanjutnya P5 dan P1 berbeda sangat nyata (P<0.05) dengan P2 8%, P3 6% dan P4 12%. Viabilitas pasca thawing menunjukkan P5 65,4% tidak berbeda nyata (P>0,05) dengan P1 61,8%, akan tetapi P5 dan P1 berbeda sangat nyata (P<0.05) dengan P2 26,2%, P3 29,8%, dan P4 34%. Membran plasma utuh (MPU) pasca thawing menunjukkan P5 66,2% tidak berbeda nyata (P>0,05) dengan P1 65,4%, akan tetapi keduanya berbeda sangat nyata (P<0.05) dengan P2 39%, P3 38%, dan P4 36,2%. Disimpulkan kriopreservasi semen kambing boer dengan pengencer nira aren dan gliserol pada konsentrasi berbeda belum dapat dipergunakan sebagai sumber bibit berdasarkan standar nasional Indonesia.Kata Kunci : Kambing boer, semen, nira arenABSTRACTThe experiment was conducted to determine the effect of sugar palm juice as alternative extender for cryopreservation process of boer semen.Tris-glycerol-egg yolk (P1 73-7-20%), Sugar palm juice-glyserol-egg yolk (P2 74-6-20%, P373-7-20%, dan P4 72-8-20%), and andromed (P5) used as a extender in the cryopreservation process of boer semen. Sperm motility (%), live sperm (%) and sperm membrane integrity (%) were recorded after diluted, equilibration and freeze-thawing. Result of post thawing motility showed that P5 52% was significantly different (P <0.05) with P1 42%, then P5 and P1 were significantly different (P <0.05) with P2 8%, P3 6% and P4 12%. Viability after thawing showed P5 65.4% was not significantly different (P> 0.05) with P1 61.8%, but P5 and P1 significantly different (P <0.05) with P2 26.2%, P3 29.8 %, and P4 34%. Spermmembrane integrity post-thawing showed P5 66.2% was not significantly different (P> 0.05) with P1 65.4%, but both were very significantly different (P <0.05) with P2 39%, P3 38% and P4 36.2%. Conclusions, sugar palm juice-glycerol-egg yolk with differentconcentrationsineffectively as an alternative extenderin cryopreservation of boer semen.Keywords: boer goat, semen, sugar palm juice

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