Characterization of a newly discovered T-cell receptor β-chain heterodimer expressed on a CD8+ bone marrow subpopulation that promotes allogeneic stem cell engraftment

10.1038/78667 ◽  
2000 ◽  
Vol 6 (8) ◽  
pp. 904-909 ◽  
Author(s):  
Matthew J. Schuchert ◽  
Renee D. Wright ◽  
Yolonda L. Colson
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Cell Engraftment ◽  
Β Chain

Clinical stem-cell sources contain CD8+CD3+ T-cell receptor–negative cells that facilitate bone marrow repopulation with hematopoietic stem cells

Blood ◽  
2008 ◽  
Vol 111 (3) ◽  
pp. 1735-1738 ◽  
Author(s):  
Stephanie Bridenbaugh ◽  
Linda Kenins ◽  
Emilie Bouliong-Pillai ◽  
Christian P. Kalberer ◽  
Elena Shklovskaya ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Hematopoietic Stem ◽  
Cd8 Cells ◽  
Human Cd34 ◽  
Cd34 Cells

Abstract Clinical observations in patients undergoing bone marrow transplantation implicate the involvement of CD8+ cells in promoting the stem-cell engraftment process. These findings are supported by mouse transplant studies, which attributed the engraftment-facilitating function to subpopulations of murine CD8+ cells, but the analogous cells in humans have not been identified. Here, we report that clinical stem-cell grafts contain a population of CD8α+CD3ϵ+ T-cell receptor– negative cells with an engraftment facilitating function, named candidate facilitating cells (cFCs). Purified cFC augmented human hematopoiesis in NOD/SCID mice receiving suboptimal doses of human CD34+ cells. In vitro, cFCs cocultured with CD34+ cells increased hematopoietic colony formation, suggesting a direct effect on clonogenic precursors. These results provide evidence for the existence of rare human CD8+CD3+TCR− cells with engraftment facilitating properties, the adoptive transfer of which could improve the therapeutic outcome of stem-cell transplantation.

T-cell Receptor β Chain Variability in Bone Marrow and Peripheral Blood in Severe Acquired Aplastic Anemia

1997 ◽  
Vol 23 (1) ◽  
pp. 110-122 ◽  
Author(s):  
Chantal Y. Manz ◽  
Pierre-Yves Dietrich ◽  
Valérie Schnuriger ◽  
Catherine Nissen ◽  
Aleksandra Wodnar-Filipowicz
Keyword(s):  
Bone Marrow ◽  
T Cell ◽  
Aplastic Anemia ◽  
T Cell Receptor ◽  
Peripheral Blood ◽  
Cell Receptor ◽  
Β Chain

Characterization of a precursor T-cell line (THP-6) with rearranged T-cell receptor β chain gene

1988 ◽  
Vol 12 (3) ◽  
pp. 227-232 ◽  
Author(s):  
Masayoshi Minegishi ◽  
Shigeru Tsuchiya ◽  
Naoko Minegishi ◽  
Osamu Yoshie ◽  
Masataka Nakamura ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Line ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Chain Gene ◽  
Β Chain

TCRpair: prediction of functional pairing between HLA-A*02:01-restricted T cell receptor α and β chains

2021 ◽  
Author(s):  
Anja Mösch ◽  
Dmitrij Frishman
Keyword(s):  
Amino Acids ◽  
T Cell ◽  
T Cell Receptor ◽  
Mrna Level ◽  
Cell Receptor ◽  
Supplementary Information ◽  
Supplementary Data ◽  
Β Chain ◽  
Complementarity Determining Region

Abstract Summary The ability of a T cell to recognize foreign peptides is defined by a single α and a single β hypervariable complementarity determining region (CDR3), which together form the T cell receptor (TCR) heterodimer. In ∼30%-35% of T cells, two α chains are expressed at the mRNA level but only one α chain is part of the functional TCR. This effect can also be observed for β chains, although it is less common. The identification of functional α/β chain pairs is instrumental in high-throughput characterization of therapeutic TCRs. TCRpair is the first method that predicts whether an α and β chain pair forms a functional, HLA-A*02:01 specific TCR without requiring the sequence of a recognized peptide. By taking additional amino acids flanking the CDR3 regions into account, TCRpair achieves an AUC of 0.71. Availability TCRpair is implemented in Python using TensorFlow 2.0 and is freely available at https://www.github.com/amoesch/TCRpair Supplementary information Supplementary data are available at Bioinformatics online.

scholarly journals Characterization of a Single-chain Antibody to the β-Chain of the T Cell Receptor

1995 ◽  
Vol 270 (43) ◽  
pp. 25819-25826 ◽  
Author(s):  
Bryan K. Cho ◽  
Beth A. Schodin ◽  
David M. Kranz
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Β Chain

Characterization of monoclonal antibodies specific for the Vβ3 family of the human T cell receptor generated using soluble TCR β-chain

1993 ◽  
Vol 164 (2) ◽  
pp. 233-244 ◽  
Author(s):  
Susan D. Calaman ◽  
Gerald R. Carson ◽  
Larry D. Henry ◽  
Jeffrey S. Kubinec ◽  
Rolf E. Kuestner ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Human T Cell ◽  
Β Chain

Characterization of a novel human T cell receptor β chain variable region family by transspecies DNA hybridization

1994 ◽  
Vol 24 (3) ◽  
pp. 641-645 ◽  
Author(s):  
Margaret A. Hall ◽  
Emma E. M. Jaeger ◽  
Ronald E. Bontrop ◽  
Jerry S. Lanchbury
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Dna Hybridization ◽  
Variable Region ◽  
Cell Receptor ◽  
Human T Cell ◽  
Β Chain
Sign in / Sign up

Export Citation Format

Share Document