T-cell Receptor β Chain Variability in Bone Marrow and Peripheral Blood in Severe Acquired Aplastic Anemia

1997 ◽  
Vol 23 (1) ◽  
pp. 110-122 ◽  
Author(s):  
Chantal Y. Manz ◽  
Pierre-Yves Dietrich ◽  
Valérie Schnuriger ◽  
Catherine Nissen ◽  
Aleksandra Wodnar-Filipowicz
Keyword(s):  
Bone Marrow ◽  
T Cell ◽  
Aplastic Anemia ◽  
T Cell Receptor ◽  
Peripheral Blood ◽  
Cell Receptor ◽  
Β Chain

T-cell receptor variable region of the β-chain gene use in peripheral blood and multiple synovial membranes during rheumatoid arthritis

1995 ◽  
Vol 42 (4) ◽  
pp. 331-339 ◽  
Author(s):  
Antoine Alam ◽  
Jacqueline Lulé ◽  
Héléne Coppin ◽  
Nathalie Lambert ◽  
Bernard Maziéres ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
T Cell ◽  
T Cell Receptor ◽  
Peripheral Blood ◽  
Variable Region ◽  
Cell Receptor ◽  
Chain Gene ◽  
Synovial Membranes ◽  
Β Chain

Prognostic value of T-cell receptor γ rearrangement in peripheral blood or bone marrow of patients with peripheral T-cell lymphomas

2008 ◽  
Vol 49 (2) ◽  
pp. 237-246 ◽  
Author(s):  
Christian SchüTzinger ◽  
Harald Esterbauer ◽  
Gregor Hron ◽  
Cathrin Skrabs ◽  
Martin Uffmann ◽  
...  
Keyword(s):  
Bone Marrow ◽  
T Cell ◽  
T Cell Receptor ◽  
Peripheral Blood ◽  
Prognostic Value ◽  
Cell Receptor ◽  
T Cell Lymphomas ◽  
Peripheral T Cell Lymphomas

A Longitudinal Analysis of the T-Cell Receptor Vβ Repertoire in Aplastic Anemia Patients at Initial Presentation, Remission, and Relapse.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 2402-2402
Author(s):  
Yunfeng Cheng ◽  
Yong Tang ◽  
Spencer Green ◽  
Keyvan Keyvanfar ◽  
Tullia Bruno ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Flow Cytometry ◽  
T Cell ◽  
Aplastic Anemia ◽  
T Cell Receptor ◽  
Immunosuppressive Treatment ◽  
Cell Receptor ◽  
Initial Presentation ◽  
Cell Counts ◽  
Therapeutic Decisions

Abstract Aplastic anemia is a bone-marrow-failure syndrome characterized by low blood-cell counts and a fatty bone marrow. In most cases, no obvious etiological factor can be identified, but clinical responses to immunosuppressive treatment (IST) strongly suggest an immune pathophysiology. Our previous study of T-cell receptor (TCR) Vβ (variable region of β-chain) repertoire usage by flow cytometry suggested that aplastic anemia results from antigen-specific lymphocyte attack on hematopoietic progenitors (Risitano et al. Lancet2004; 364:355). In the current work, 7 patients were investigated for Vβ pattern expression before first immunosuppresive treatment, at the remission, and again on relapse. The TCR Vβ repertoire was analyzed for CD4+ and CD8+ subsets, separately, by flow cytometry, using a monoclonal antibody set of 22 different Vβ chains. Most patients had very different patterns of Vβ usage from healthy individuals, and all but one showed expansion of at least one Vβ family before immunosuppressive treatment (Vβ family expansions were defined as 2 standard deviations (SD) from the means in controls). The median number of expanded Vβ families was 4 per patient among CD8CD28dim effector cells. At remission, almost all the initially expanded Vβ subfamilies decreased to less than 2SD of controls. At relapse, most of the expanded Vβ subsets were increased again. However, 5/7 patients showed new expanded Vβ subsets at recurrence of cytopenias, suggesting antigenic spread of new epitopes recognized by immune systems. Although no common pattern of specific expanded Vβ subsets could be identified among different patients, some Vβ subfamilies appeared to be more frequently involved (Vβ 5.1 and Vβ 5.2 were expanded in 4 of 7 patients both at initial presentation and relapse ). These data suggest that monitoring Vβ subsets in aplastic anemia, and potentially in other immune-mediated human diseases of a similar pathophysiology could be used to guide individual therapeutic decisions and in the development of new treatments.

T-Cell Expansions With Conserved T-Cell Receptor β Chain Motifs in the Peripheral Blood of HLA-DRB1*0401 Positive Patients With Necrotizing Vasculitis

Blood ◽  
1998 ◽  
Vol 92 (10) ◽  
pp. 3737-3744 ◽  
Author(s):  
Johan Grunewald ◽  
Eva Halapi ◽  
Jan Wahlström ◽  
Ricardo Giscombe ◽  
Soniya Nityanand ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Receptor ◽  
Peripheral Blood ◽  
Cd4 T Cells ◽  
Specific Antigen ◽  
Cell Receptor ◽  
Antigenic Peptides ◽  
Cdr3 Region ◽  
Β Chain

T lymphocytes are implicated in the pathogenesis of systemic vasculitis such as Wegener’s granulomatosis (WG) and polyarteritis nodosa (PAN). In the present study, we have characterized in detail the T-cell receptor (TCR) of peripheral blood T cells from eight vasculitis patients of known HLA class II genotypes. We used flow cytometry to outline the exact TCR V gene expression, complementarity determining region 3 (CDR3) fragment analysis to estimate the degree of clonality and cDNA sequencing to define the exact TCR  or β chain sequences. The TCR CDR3 region interacts with antigenic peptides presented by HLA molecules, and it is normally immensely diverse. It was therefore of particular interest to identify a common dominating TCR BV8-F/L-G-G-A/Q-G-J2S3 β chain sequence in the CD4+T cells of four unrelated vasculitis patients. Furthermore, this BV8-associated CDR3 motif was linked to the HLA-DRB1*0401 allele, as well as to active disease and/or an established BV8+ CD4+ T-cell expansion. In contrast, age- and HLA-matched patients with rheumatoid arthritis did not harbor the described BV8 motif. These results strongly suggest that BV8+ CD4+ T cells with the described CDR3 motif recognize a specific antigen presented by DR4 molecules, indicating the existence of a common vasculitis-associated antigen.

scholarly journals Characteristics of profiling the peripheral blood T-cell receptor β-chain repertoire in gastric cancer

2020 ◽  
Author(s):  
YANWEN LIU YANWEN LIU ◽  
Nan nan Guo Nan nan Guo ◽  
Yi ting Wu Yi ting Wu ◽  
Yu hua Shi Yu hua Shi
Keyword(s):  
Gastric Cancer ◽  
T Cell ◽  
Advanced Gastric Cancer ◽  
Cancer Patients ◽  
T Cell Receptor ◽  
Peripheral Blood ◽  
Cell Receptor ◽  
Significant Difference ◽  
Tcr Repertoire ◽  
Β Chain

Abstract Background: Gastric cancer is the third cause of cancer-related deaths worldwide, and is initially detected and attacked by the immune system through tumor-reactive T cells. The aim of this study was to determine the basic characteristics of the peripheral blood T-cell receptor (TCR) repertoire in patients with gastric cancer. Methods: High throughput sequencing was used to identify hyper-variable rearrangements of complementarity determining region 3 (CDR3) of the TCR β chain to comprehensively profile the TCR repertoire in peripheral blood samples from 6 advanced gastric cancer patients and 3 early gastric cancer controls. Results: The study showed that the TCR repertoire differed substantially between advanced cancer patients and early controls in terms of CDR3 clonotype, diversity and V/J segment usage. Specifically, low diversity reflected a worse immune status and prognosis in advanced gastric cancer. However the diversity of TCR was not significant difference in wild or mutation patients. Conclusion: TCR repertoire analysis served as a useful indicator of disease development and prognosis in gastric cancer and may be utilized to be biomarker for immunotherapy.

Detection of Putative T cell Clones Using T cell Receptor β Chain Gene Clonality Assay in Korean Patients with Aplastic Anemia

2009 ◽  
Vol 29 (4) ◽  
pp. 269-276 ◽  
Author(s):  
Hyun-Jung Choi ◽  
Myung-Geun Shin ◽  
Hye-Ran Kim ◽  
Hyeoung-Joon Kim ◽  
Hoon Kook ◽  
...  
Keyword(s):  
T Cell ◽  
Aplastic Anemia ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Chain Gene ◽  
T Cell Clones ◽  
Korean Patients ◽  
Cell Clones ◽  
Β Chain
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